| Project/Area Number |
23591002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | 独立行政法人国立病院機構 下志津病院(臨床研究部) |
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Principal Investigator |
TOMIZAWA Minoru 独立行政法人国立病院機構下志津病院(臨床研究部), その他部局等, その他 (90334193)
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| Co-Investigator(Renkei-kenkyūsha) |
OHIRA Miki 千葉県がんセンター(研究所), がんゲノムセ ンター・がんゲノム研究室, 室長 (20311384)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | iPS cells / 肝細胞 / 転写因子 / 増殖因子 / 糖新生 / 尿素サイクル / iPS細胞 / cDNAマイクロアレイ / ブドウ糖 / アルギニン / 肝前駆細胞 / リアルタイム定量PCR |
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| Research Abstract |
A medium with Activin A was developed for feeder-free culture of iPS cells. iPS cells differentiated to hepatoblasts from iPS cells transfected with C/EBP alpha, GATA4, HEX, and FoxA2 in a medium with Oncostatin M, EGF, and RA. Hepatocyte selection medium was developed without glucose or arginine, supplemented with galactose and ornithine. When HSM was added to co-culture of iPS cells and human primary hepatocytes, iPS cells died and hepatocytes survived. Hepatocytes were successfully purified.
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