Rho-kinase activity in circulating neutrophils as a useful biomarker for diagnosis and disease activity assessment of vasospastic angina

• Project/Area Number: 23591026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: National Cardiovascular Center Research Institute (2012-2013); Tohoku University (2011)
• Principal Investigator: YASUDA Satoshi 独立行政法人国立循環器病研究センター, 病院, 部門長 (00431578)
• Co-Investigator(Kenkyū-buntansha): SHIMOKAWA Hiroaki 東北大学, 大学院医学系研究科, 教授 (00235681) ; TAKAHASHI Jun 東北大学, 大学院医学系研究科, 講師 (00375081)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ストレス / 循環器・高血圧 / トランスレーショナルリサーチ

Research Abstract

We examined whether Rho-kinase activity is systemically enhanced in vasospastic angina (VSA) patients. The Rho-kinase activity was significantly higher in the VSA group than in the non-VSA group. We also found that Rho-kinase activity of VSA patients was transiently enhanced associated with disaster-related mental stress and that Rho-kinase activity exhibits distinct circadian variation associated with alterations in coronary vasomotor responses. These results indicate that Rho-kinase activity in circulating neutrophils may be a useful biomarker for diagnosis and disease activity assessment of the vasospastic disorder.

Research Products

• [Journal Article] Circadian Variation of Rho-Kinase Activity in Circulating Leukocytes of Patients With Vasospastic Angina (2014)
  • Author(s): Nihei T, Takahashi J, Tsuburaya R, Ito Y, Shiroto T, Hao K, Takagi Y, Matsumoto Y, Nakayama M, Miyata S, Sakata Y, Ito K, Shimokawa H.
  • Journal Title: Circ J Volume: Mar 27(Epub ahead of print)
  • NAID: 130003391088
• [Journal Article] Contemporary crossing techniques for infrapopliteal chronic total occlusions (2014)
  • Author(s): Kawarada O, Sakamoto S, Harada K, Ishihara M, Yasuda S, Ogawa H.
  • Journal Title: J Endovasc Ther Volume: 21 Pages: 266-80
• [Journal Article] Vasospastic limb ischemia presenting acute and chronic limb ischemia (2014)
  • Author(s): Kaneyama J, Kawarada O, Sakamoto S, Harada K, Ishihara M, Yasuda S, Hisao O.
  • Journal Title: Ann Vasc Dis Volume: (in press)
  • NAID: 130004488034
• [Journal Article] Circadian Variation of Rho-Kinase Activity in Circulating Leukocytes of Patients With Vasospastic Angina. (2014)
  • Author(s): Nihei T, Takahashi J, Tsuburaya R, Ito Y, Shiroto T, Hao K, Takagi Y, Matsumoto Y, Nakayama M, Miyata S, Sakata Y, Ito K, Shimokawa H.
  • Journal Title: Circ J. Volume: in press
  • NAID: 130003391088
  • Peer Reviewed
• [Journal Article] Contemporary crossing techniques for infrapopliteal chronic total occlusions. (2014)
  • Author(s): Kawarada O, Sakamoto S, Harada K, Ishihara M, Yasuda S, Ogawa H.
  • Journal Title: J Endovasc Ther Volume: 21 Pages: 266-80
  • Peer Reviewed
• [Journal Article] Vasospastic limb ischemia presenting acute and chronic limb ischemia. (2014)
  • Author(s): Kaneyama J, Kawarada O, Sakamoto S, Harada K, Ishihara M, Yasuda S, Ogawa H.
  • Journal Title: Ann Vasc Dis Volume: in press
  • NAID: 130004488034
  • Peer Reviewed
• [Journal Article] Enhanced Rho-kinase activity in patients with vasospastic angina after the Great East Japan Earthquake (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Takagi Y, Hao K, Tsuburaya R, Shiroto T, Ito Y, Matsumoto Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Journal Title: Circ J Volume: 76 Pages: 2892-4
  • NAID: 10031126063
• [Journal Article] Enhanced Rho-kinase activity in patients with vasospastic angina after the Great East Japan Earthquake. (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Takagi Y, Hao K, Tsuburaya R, Shiroto T, Ito Y, Matsumoto Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Journal Title: Circ J Volume: 76 Pages: 2892-4
  • NAID: 10031126063
  • Peer Reviewed
• [Journal Article] Enhanced Rho-kinase activity in circulating neutrophils of patients with vasospastic angina : a possible biomarker for diagnosis and disease activity assessment (2011)
  • Author(s): Kikuchi Y, Yasuda S, Aizawa K, Tsuburaya R, Ito Y, Takeda M, Nakayama M, Ito K, Takahashi J, Shimokawa H.
  • Journal Title: J Am Coll Cardiol Volume: 58 Pages: 1231-7
• [Journal Article] Enhanced Rho-kinase activity in circulating neutrophils of patients with vasospastic angina: a possible biomarker for diagnosis and disease activity assessment. (2011)
  • Author(s): Kikuchi Y, Yasuda S, Aizawa K, Tsuburaya R, Ito Y, Takeda M, Nakayama M, Ito K, Takahashi J, Shimokawa H.
  • Journal Title: J Am Coll Cardiol. Volume: 58 Pages: 1231-7
  • Peer Reviewed
• [Presentation] Circadian Variation of Rho-kinase Activity in Circulating Leukocytes in Patients with Vasospastic Angina Circulation Journal 2014, 78(Suppl. I) : I-597 (2014)
  • Author(s): Taro Nihei, Jun Takahashi, Yoku Kikuchi, Kiyotaka Hao, Yusuke Takagi, Ryuji Tsuburaya, Takashi Shirato, Yoshitaka Itoh, Yasuharu Matsumoto, Masaharu Nakayama, Kenta Ito, Hiroaki Shimokawa
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: 東京
• [Presentation] Circadian Variation of Rho-kinase Activity in Circulating Leukocytes in Patients with Vasospastic Angina (2014)
  • Author(s): Taro Nihei,Jun Takahashi, Yoku Kikuchi, Kiyotaka Hao, Yusuke Takagi, Ryuji Tsuburaya, Takashi Shirato, Yoshitaka Itoh, Yasuharu Matsumoto, Masaharu Nakayama, Kenta Ito, Hiroaki Shimokawa
  • Organizer: 第78回日本循環器学会学術集会
  • Place of Presentation: 東京、日本
• [Presentation] Circadian variation of Rho-kinase activity associated with enhanced coronary reactivity in patients with vasospastic angina (2013)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Tsuburaya R, Shiroto T, Ito Y, Matsumoto Y, Nakayama Y, Ito K, Shimokawa H.
  • Organizer: European Society of Cardiology 2013
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] Circadian variation of Rho-kinase activity associated with enhanced coronary reactivity in patients with vasospastic angina (2013)
  • Author(s): .Nihei T, Takahashi J, Kikuchi Y, Tsuburaya R, Shiroto T, Ito Y, Matsumoto Y, Nakayama Y, Ito K, Shimokawa H.
  • Organizer: uropean Society of Cardiology 2013
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] Enhanced rho-kinase activity in circulating neutrophils of patients with vasospasticangina after the Great East Japan Earthquake. (#9574) (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Hao K, Takagi Y, Tsuburaya R, Shiroto T, ItoY, Matsumoto Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Organizer: American Heart Association (AHA) Scientific Sessions
  • Place of Presentation: Los Angeles, USA
• [Presentation] Increased Rho-kinase activity in patients with vasospastic angina after the Great East Japan Earthquake disaster (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Ito Y, Tsuburaya R, Takagi Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Organizer: European Society of Cardiology Congress 2012
  • Place of Presentation: Munich, Germany
• [Presentation] Enhanced rho-kinase activity in circulating neutrophils of patients with vasospasticangina after the Great East Japan Earthquake. (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Hao K, Takagi Y, Tsuburaya R, Shiroto T, ItoY, Matsumoto Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Organizer: American Heart Association
  • Place of Presentation: Los Angeles, USA
• [Presentation] Increased Rho-kinase activity in patients with vasospastic angina after the Great East Japan Earthquake disaster. (2012)
  • Author(s): Nihei T, Takahashi J, Kikuchi Y, Ito Y, Tsuburaya R, Takagi Y, Nakayama M, Ito K, Yasuda S, Shimokawa H.
  • Organizer: European Society of Cardiology Congress
  • Place of Presentation: Munich, Germany
• [Presentation] Usefulness of Rho-kinase activity in circulating neutrophils for disease activity assessment of vasospastic angina. (2011)
  • Author(s): 9.Kikuchi Y, Aizawa K, Yasuda S, Tsuburaya R, Ito Y, Takeda M, Nakayama M, Ito K, Takahashi J, Shimokawa H.
  • Organizer: American Heart Association (AHA) Scientific Sessions
  • Place of Presentation: Chicago, USA
• [Book] The Pathogenesis of Vasospastic Angina. In : Kaski JC, Eslick GD, Bairey Merz CN (eds.), Chest Pain with Normal Coronary Arteries (2013)
  • Author(s): Yasuda S, Shimokawa H.
  • Publisher: Springer-Verlag
• [Book] The Pathogenesis of Vasospastic Angina. In: Kaski JC, Eslick GD, Bairey Merz CN (eds.) (2013)
  • Author(s): 2.Yasuda S, Shimokawa H.
  • Total Pages: 12
  • Publisher: Springer-Verlag, London, UK
• [Remarks] 東北大学循環器内科
  • URL: http://www.cardio.med.tohoku.ac.jp/index.html
• [Remarks] 国立循環器病研究センター病院
  • URL: http://hospital.ncvc.go.jp/index.html
• [Remarks]
  • URL: http://www.cardio.med.tohoku.ac.jp/class/achievements/index.html