Translational research of circulation-regulated peptide using B-type natriuretic peptide and adrenomedullin

• Project/Area Number: 23591041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto University
• Principal Investigator: NISHIKIMI Toshio 京都大学, 医学(系)研究科(研究院), その他 (80291946)
• Co-Investigator(Kenkyū-buntansha): KUWAHARA Koichiro 京都大学, 医学部付属病院医学部付属病院内分泌代謝内科, 講師 (30402887) ; NAKAGAWA Yasuaki 京都大学, 内分泌代謝内科, 助教 (70452357)
• Co-Investigator(Renkei-kenkyūsha): MINAMINO Naoto 国立循環器病研究センター研究所, 薬理部, 部長 (50124839)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: B 型ナトリウム利尿ペプチド / アドレノメデュリン / 循環ペプチド / 心不全 / ノックアウトマウス / proBNP / 脂肪組織 / BNP / プロセシング / 糖鎖修飾 / 血圧 / 糖鎖 / 脂肪 / processing

Research Abstract

We investigated the translational research of circulation-regulated peptide using B-type natriuretic peptide and adrenomedullin. We newly developed the immunofluorescent assay for proBNP and total BNP(= BNP32 + proBNP). Using this immunofluorescent assay system we found that proBNP is about 70% of total BNP immunoreactivity. ProBNP level increases with age. ProBNP level is increased in heart failure in proportion to its severity. ProBNP/total BNP ratios are greater in chronic renal failure patients than in normal subjects. We successfully produced fat tissue specific deficient AM knockout mouse. The phenotype under normal diet reveals no differences compared with wild type mouse such as body weight, body height, and fat tissue amount.

Research Products

• [Journal Article] Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis (2014)
  • Author(s): Wetzel-Strong SE, Nishikimi T, 他3名
  • Journal Title: Dev Dyn Volume: 243 Pages: 243-56
  • Peer Reviewed
• [Journal Article] The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis. (2014)
  • Author(s): Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Nakao K, Minami T, Yamada C, Ueshima K, Ikeda Y, Okamoto H, Horii K, Nagata K, Kangawa K, Minamino N, Nakao K
  • Journal Title: PLoS One Volume: 9
  • NAID: 120007100132
  • Peer Reviewed
• [Journal Article] Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis (2014)
  • Author(s): Wetzel-Strong SE, Li M, Klein KR, Nishikimi T, Caron KM
  • Journal Title: Dev Dyn Volume: 243 Pages: 243-256
  • Peer Reviewed
• [Journal Article] Complexity of molecular forms of B-type natriuretic peptide in heart failure (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Minamino N, 他2名
  • Journal Title: Heart Volume: 99 Pages: 677-9
  • Peer Reviewed
• [Journal Article] Direct immunochemiluminescent assay for proBNP and total BNP in human plasma proBNP and total BNP levels in normal and heart failure (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Minamino N, 他13名
  • Journal Title: PLoS One Volume: 8
  • NAID: 120005241437
  • Peer Reviewed
• [Journal Article] Increased expression of HCN channels in the ventricular myocardium contributes to enhanced arrhythmicity in mouse failing hearts (2013)
  • Author(s): Kuwabara Y, Kuwahara K, Nakagawa Y, Nishikimi T, 他13名
  • Journal Title: J Am Heart Assoc Volume: 2
  • Peer Reviewed
• [Journal Article] Adrenomedullin in cardiovascular disease : a useful biomarker, its pathological roles and therapeutic application (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, 他2名
  • Journal Title: Curr Protein Pept Sci Volume: 14 Pages: 256-67
  • Peer Reviewed
• [Journal Article] Angiotensin II type 1a receptor signalling directly contributes to the increased arrhythmogenicity in cardiac hypertrophy (2013)
  • Author(s): Yasuno S, Kuwahara K, Nakagawa Y, Nishikimi T, 他7名
  • Journal Title: Br J Pharmacol Volume: 170 Pages: 1384-95
  • Peer Reviewed
• [Journal Article] ストローマとナトリウム利尿ペプチド-急性心筋梗塞の病態生理的意義と臨床応用- (2013)
  • Author(s): 錦見俊雄
  • Journal Title: 循環器内科 Volume: 74 Pages: 174-180
• [Journal Article] Complexity of molecular forms of B-type natriuretic peptide in heart failure (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Kangawa K, Minamino N, Nakao K
  • Journal Title: Heart Volume: 99 Pages: 677-679
  • Peer Reviewed
• [Journal Article] Direct immunochemiluminescent assay for proBNP and total BNP in human plasma proBNP and total BNP levels in normal and heart failure. (2013)
  • Author(s): Nishikimi T, Okamoto H, Nakamura M, Ogawa N, Horii K, Nagata K, Nakagawa Y, Kinoshita H, Yamada C, Nakao K, Minami T, Kuwabara Y, Kuwahara K, Masuda I, Kangawa K, Minamino N, Nakao K
  • Journal Title: PLoS One Volume: 8
  • NAID: 120005241437
  • Peer Reviewed
• [Journal Article] Adrenomedullin in cardiovascular disease: a useful biomarker, its pathological roles and therapeutic application (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Kangawa K, Nakao K
  • Journal Title: Curr Protein Pept Sci Volume: 14 Pages: 256-267
  • Peer Reviewed
• [Journal Article] Direct Immunochemiluminescent Assay for proBNP and Total BNP in Human Plasma proBNP and Total BNP Levels in Normal and Heart Failure (2013)
  • Author(s): Nishikimi, T., Okamoto, H., Nakamura, M., Ogawa, N., Horii, K., Nagata, K., Nakagawa, Y., Kinoshita, H., Yamada, C., Nakao, K., Minami, T., Kuwabara, Y., Kuwahara, K., Masuda, I., Kangawa, K., Minamino, N., Nakao, K.
  • Journal Title: PLoS ONE Volume: 8(1) Issue: 1 Pages: e53233-e53233
  • DOI: 10.1371/journal.pone.0053233
  • NAID: 120005241437
  • Peer Reviewed
• [Journal Article] Complexity of molecular forms of B-type natriuretic peptide in heart failure. (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Kangawa K, Minamino N, Nakao K.
  • Journal Title: HEART Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Adrenomedullin in cardiovascular disease: a useful biomarker, its pathological roles and therapeutic application. (2013)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, Nakao K, Kangawa K
  • Journal Title: Curr. Protein & Peptide Sci Volume: 印刷中
  • Peer Reviewed
• [Journal Article] BNPの分子型の最新知見-BNPの生化学、分子生物学、臨床的意義を含めて- (2013)
  • Author(s): 錦見俊雄
  • Journal Title: シーメンスサイエンティフィックインフォメーション Volume: 16 Pages: 1-9
• [Journal Article] The effect of glycosylation on plasma N-terminal proBNP-76 levels in patients with heart or renal failure (2012)
  • Author(s): Nishikimi T, Kuwahara K, Nakagawa Y, 他7名
  • Journal Title: Heart Volume: 98 Pages: 152-61
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the fetal cardiac gene program (2012)
  • Author(s): Kuwahara K, Nishikimi T, Nakao K
  • Journal Title: J Pharmacol Sci Volume: 119 Pages: 198-203
  • NAID: 10030986728
  • Peer Reviewed
• [Journal Article] Do plasma levels of brain natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP) increase in diastolic dysfunction as well as in systolic dysfunction? (2012)
  • Author(s): Nishikimi T
  • Journal Title: Circ J Volume: 76 Pages: 2540-1
  • NAID: 10031078464
  • Peer Reviewed
• [Journal Article] Reciprocal expression of MRTF-A and myocardin is crucial for pathological vascular remodelling in mice (2012)
  • Author(s): Minami T, Kuwahara K, Nakagawa Y, Nishikimi T, 他18名
  • Journal Title: EMBO J Volume: 31 Pages: 4428-40
  • Peer Reviewed
• [Journal Article] 心疾患のバイオマーカーとしてのBNPの意義ー基礎的・臨床的観点からー (2012)
  • Author(s): 錦見俊雄
  • Journal Title: 日本臨床 Volume: 70 Pages: 774-784
• [Journal Article] BNP、ANPと高血圧 (2012)
  • Author(s): 錦見俊雄
  • Journal Title: 血圧 Volume: 19 Pages: 884-889
• [Journal Article] 降圧薬としてのエンドセリン拮抗薬、PDE5阻害薬—治療抵抗性高血圧におけるエンドセリン拮抗薬、PDE5阻害薬の可能性— (2012)
  • Author(s): 錦見俊雄
  • Journal Title: 循環器内科 Volume: 72 Pages: 515-520
  • NAID: 40019499216
• [Journal Article] Reciprocal expression of MRTF-A and myocardin is crucial for pathological vascular remodeling in mice. (2012)
  • Author(s): Minami T, Nakao K, et al
  • Journal Title: EMBO J. Volume: 31 Issue: 23 Pages: 4428-4440
  • DOI: 10.1038/emboj.2012.296
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the fetal cardiac gene program. (2012)
  • Author(s): Kuwahara K, Nishikimi T, Nakao K
  • Journal Title: J Pharmacol Sci Volume: 119 Pages: 198-203
  • NAID: 10030986728
  • Peer Reviewed
• [Journal Article] 降圧薬としてのエンドセリン拮抗薬、PDE５阻害薬―治療抵抗性高血圧におけるエンドセリン拮抗薬、PDE５阻害薬の可能性 (2012)
  • Author(s): 錦見俊雄
  • Journal Title: 循環器内科 Volume: 72 Pages: 42-47
  • NAID: 40019499216
• [Journal Article] The effect of glycosylation on plasma N-terminal proBNP-76 levels in patients with heart or renal failure (2012)
  • Author(s): Nishikimi T, Nakao K, et al
  • Journal Title: Heart Volume: 98 Issue: 2 Pages: 152-161
  • DOI: 10.1136/heartjnl-2011-300102
  • Peer Reviewed
• [Journal Article] 心疾患のバイオマーカーとしてのBNPの臨床的意義―基礎的臨床的観点からー (2012)
  • Author(s): 錦見俊雄
  • Journal Title: 日本臨床 Volume: 70 Pages: 774-784
• [Journal Article] Diverse molecular forms of plasma B-type natriuretic peptide in heart failure (2011)
  • Author(s): Nishikimi T, Minamino N, Nakao K
  • Journal Title: Curr Heart Fail Rep Volume: 8 Pages: 140-6
  • NAID: 120003184065
  • Peer Reviewed
• [Journal Article] Current biochemistry, molecular biology, and clinical relevance of natriuretic peptides (2011)
  • Author(s): Nishikimi T, Kuwahara K, Nakao K
  • Journal Title: J Cardiol Volume: 57 Pages: 131-40
  • NAID: 10031159554
  • Peer Reviewed
• [Journal Article] Zinc-finger protein 90 negatively regulates neuron- restrictive silencer factor-mediated transcriptional repression of fetal cardiac genes (2011)
  • Author(s): Hata L, Kuwahara K, Nakagawa Y, Nishikimi T, 他12名
  • Journal Title: J Mol Cell Cardiol Volume: 50 Pages: 972-81
  • Peer Reviewed
• [Journal Article] Japanese-Western consensus meeting on biomarkers (2011)
  • Author(s): Maisel AS, Minamino N, Nishikimi T, 他12名
  • Journal Title: Int Heart J Volume: 52 Pages: 253-65
  • Peer Reviewed
• [Journal Article] Diverse molecular forms of plasma B-type natriuretic peptide in heart failure (2011)
  • Author(s): Nishikimi T, Minamino N, Nakao K
  • Journal Title: Curr Heart Fail Rep 2011;8:140-6 Volume: 8 Issue: 2 Pages: 140-146
  • DOI: 10.1007/s11897-011-0051-y
  • NAID: 120003184065
  • Peer Reviewed
• [Journal Article] Current biochemistry molecular biology, and clinical relevance of natriuretic peptides (2011)
  • Author(s): Nishikimi T, Kuwahara K, Nakao K
  • Journal Title: J Cardiol Volume: 57 Issue: 2 Pages: 131-140
  • DOI: 10.1016/j.jjcc.2011.01.002
  • NAID: 10031159554
  • Peer Reviewed
• [Journal Article] Japanese-Western consensus meeting on biomarkers (2011)
  • Author(s): Maisel AS, Nakao K, Ponikowski P, Peacock WF, Yoshimura M, Suzuki T, Tsutamoto T, Filippatos GS, Saito Y, Seino Y, Minamino N, Hirata Y, Mukoyama M, Nishikimi T, Nagai R
  • Journal Title: Int Heart J. Volume: 52 Pages: 253-265
  • Peer Reviewed
• [Journal Article] Zinc-finger protein 90 negatively relulates neuron-restrictive silencer factor-mediated transcriptional repression of fetal cardiac genes (2011)
  • Author(s): Hata L, Murakami M, Kuwahara K, Nakagawa Y, Kinoshita H, Usami S, Yasuno S, Fujiwara M, Kuwabara Y, Minami T, Yamada Y, Yamada C, Nakao K, Ueshima K, Nishikimi T, Nakao K
  • Journal Title: J Mol Cell Cardiol. Volume: 5 Issue: 6 Pages: 972-981
  • DOI: 10.1016/j.yjmcc.2011.01.017
  • Peer Reviewed
• [Presentation] Molecular mechanism of lower processing rate in human proBNP : importance of the distance between the O-glycosylation and cleavage sites (2014)
  • Author(s): Toshio Nishikimi, Yasuaki Nakagawa, Naoto Minamino, Koichiro Kuwahara, 他9名
  • Organizer: 日本循環器学会総会
  • Place of Presentation: 東京
• [Presentation] ナトリウム利尿ペプチドBNP、ANPの最新知見とその臨床応用 (2013)
  • Author(s): 錦見俊雄
  • Organizer: 日本自動分析学会
  • Place of Presentation: 横浜(ランチョンセミナー)
  • Year and Date: 2013-10-12
• [Presentation] B型ナトリウム利尿ペプチド研究の最前線 (2013)
  • Author(s): 錦見俊雄
  • Organizer: 日本高血圧学会総会
  • Place of Presentation: 大阪(シンポジウム)
• [Presentation] O-glycosylated Pro B-type Natriuretic Peptide is Cleaved intracellularly By Furin in Ventricular Myocytes and Atrial Myocytes : Importance of the Distance Between the O-glycosylation Sites and Cleaved Sites (2013)
  • Author(s): Toshio Nishikimi, Yasuaki Nakagawa, Naoto Minamino, Koichiro Kuwahara, 他9名
  • Organizer: American Heart Association Annual Meeting
  • Place of Presentation: Dallas, USA
• [Presentation] 心不全におけるBNP前駆体proBNPの病態生理的意義 (2013)
  • Author(s): 錦見俊雄
  • Organizer: 日本心血管内分泌代謝学会
  • Place of Presentation: 大阪(イブニングセミナー)
• [Presentation] BNP測定の特徴と値の見方 ーBNP関連ペプチドの最新知見ー (2013)
  • Author(s): 錦見俊雄
  • Organizer: The 77th Annual Scientific Meeting of The Japanese Circulation
  • Place of Presentation: パシフィコ横浜
• [Presentation] ヒト血漿におけるproBNP、total BNP直接測定法の確立ー健常者、心不全患者での検討ー (2012)
  • Author(s): 錦見俊雄、中川靖章、桑原宏一郎、南野直人, 他11名
  • Organizer: 第35回日本高血圧学会
  • Place of Presentation: 名古屋
• [Presentation] Direct immunoassay for proBNP and total BNP in human plasma–proBNP and total BNP levels in healthy and heart failure patients (2012)
  • Author(s): Nishikimi T, Nakagawa1 Y, Kuwahara K, Minamino N, 他8名
  • Organizer: 24th International Society of Hypertension
  • Place of Presentation: Sidney, Australia
• [Presentation] proBNP、total BNP直接化学発光測定法の確立ー心不全患者における検討ー (2012)
  • Author(s): 錦見俊雄、中川靖章、桑原宏一郎、南野直人, 他12名
  • Organizer: 第60回日本心臓病学会学術集会
  • Place of Presentation: 金沢
• [Presentation] BNP revisited (2012)
  • Author(s): 錦見俊雄
  • Organizer: 第16回日本心血管内分泌学会
  • Place of Presentation: 東京(ワークショップ)
• [Presentation] BNP関連ペプチドの最新知見 (2012)
  • Author(s): 錦見俊雄
  • Organizer: The76th Annual Scientific Meeting of The Japanese Circulation
  • Place of Presentation: Fukuoka, Japan(ランチョンセミナー)
• [Presentation] BNP関連ペプチドの最新知見 (2012)
  • Author(s): 錦見俊雄
  • Organizer: The76th Annual Scientific Meeting of The Japanese Circulation
  • Place of Presentation: 福岡国際会議場、福岡
• [Presentation] proBNP、total BNP直接化学発光測定法の確立ー心不全患者における検討ー (2012)
  • Author(s): 錦見俊雄
  • Organizer: 第60回日本心臓病学会学術集会
  • Place of Presentation: 金沢全日空ホテル、金沢
• [Presentation] ヒト血漿におけるproBNP、total BNP直接測定法の確立ー健常者、心不全患者での検討ー (2012)
  • Author(s): 錦見俊雄
  • Organizer: 第35回日本高血圧学会
  • Place of Presentation: ウエスティン名古屋キャッスルホテル、名古屋
• [Presentation] B-type natriuretic peptide: revisited (2012)
  • Author(s): 錦見俊雄
  • Organizer: 第16回日本心血管内分泌代謝学会
  • Place of Presentation: 東京大学構内鉄門講堂、東京
• [Presentation] BNP関連ペプチドの最新知見 (2012)
  • Author(s): 錦見俊雄
  • Organizer: The76th Annual Scientific Meeting of The Japanese Circulation Society
  • Place of Presentation: 国際会議場（福岡）
• [Presentation] Increased HCN channels in failing hearts possibly contribute to ventricular arrhythmias (2012)
  • Author(s): Y Kuwabara, K Kuwahara, M Takano, H Kinoshita, Y Nakagawa, S Usami, S Yasuno, K Nakao, T Minami, Y Yamada, J Shibata, T Nishikimi, K Ueshima and K Nakao
  • Organizer: The76th Annual Scientific Meeting of The Japanese Circulation Society
  • Place of Presentation: 国際会議場（福岡）
• [Presentation] MRTF-A, a rho-dependent co-activator of SRF, plays a critical role in vascular remodeling by regulating smooth muscle cell migration (2012)
  • Author(s): T Minami, K Kuwahara, J Shibata, C Yamada, Y Kuwabara, K Nakao, H Kinoshita, S Usami, S Yasuno, Y Nakagawa, K Ueshima, T Nishikimi, M Takaoka, M Sata, R Nagai, K Nakao
  • Organizer: The76th Annual Scientific Meeting of The Japanese Circulation Society
  • Place of Presentation: 国際会議場（福岡）
• [Presentation] TRPC3/6 Ion Channels as Potentially Novel Therapeutic Targets against Pulmonary Arterial Hypertension (2012)
  • Author(s): H Kinoshita, K Kuwahara, S Kiyonaka, Y Mori, J Shibata, C Yamada, T Minami, K Nakao, Y Kuwabara, S Yasuno, S Usami, Y Nakagawa, T Nishikimi, K Ueshima, K Nakao
  • Organizer: The76th Annual Scientific Meeting of The Japanese Circulation Society
  • Place of Presentation: 国際会議場（福岡）
• [Presentation] Analysis of circulating glycosylated N-terminal proBNP and nonglycosylated N-terminal proBNP in patients with heart failure (HF) and chronic renal failure (CRF) on hemodialysis (2011)
  • Author(s): Nishikimi T, Minamino N, Kuwahara1 K, Nakagawa Y, 他5名
  • Organizer: European Society of Cardiology 33rd Annual Congress
  • Place of Presentation: Paris, France
• [Presentation] 高血圧性左室肥大・潜在性心機能低下のサロゲートマーカーとしての血漿BNP測定の意義と問題点 (2011)
  • Author(s): 錦見俊雄
  • Organizer: 第34回日本日本高血圧学会総会
  • Place of Presentation: 宇都宮市(Up to Date)
• [Presentation] 高血圧性左室肥大・潜在性心機能低下のサロゲートマーカーとしての血漿BNP測定の意義と問題点 (2011)
  • Author(s): 錦見俊雄
  • Organizer: 第34回日本日本高血圧学会総会
  • Place of Presentation: 栃木文化センター（栃木）
• [Presentation] 拡張型心筋症・不整脈死モデルマウスにおけるN型Ca2+チャネル阻害の有用性の検討 (2011)
  • Author(s): 山田優子、桑原宏一郎、木下秀之、桑原佳宏、中川靖章、柴田純子、山田千夏、南丈也、保野慎治、宇佐美覚、中尾一泰、錦見俊雄、上嶋健治、森泰生、中尾一和
  • Organizer: 第34回日本日本高血圧学会総会
  • Place of Presentation: 栃木文化センター（栃木）
• [Presentation] BNP遺伝子発現調節領域におけるserum response factor応答領域の新たなる同定とその心筋細胞肥大反応における意義 (2011)
  • Author(s): 桑原宏一郎・木下秀之・桒原佳宏・中川靖章・南 丈也・山田千夏・柴田純子・宇佐美覚・錦見俊雄・中尾一和
  • Organizer: 第15回日本心血管内分泌学会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪）
• [Presentation] 病的血管リモデリングでみられる血管平滑筋細胞形質転換におけるRho依存性転写共役因子MRTF-Aの意義 (2011)
  • Author(s): 南 丈也・桑原宏一郎・中川靖章・木下秀之・中尾一泰・桒原佳宏・山田千夏・柴田純子・宇佐美覚・錦見俊雄・中尾一和
  • Organizer: 第15回日本心血管内分泌学会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪）
• [Presentation] ANP・BNP遺伝子発現調節因子NRSFにより制御されるHCNチャネルの心不全モデルマウスにおける突然死発症への関与 (2011)
  • Author(s): 桒原佳宏，桑原宏一郎，木下秀之，鷹野誠，中川靖章，宇佐美覚，柴田純子，山田千夏，南丈也，山田優子，藤原正隆，保野慎治，錦見俊雄，上嶋健治，中尾一和
  • Organizer: 第15回日本心血管内分泌学会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪）
• [Presentation] Analysis of circulating glycosylated N-terminal-proBNP and nonglycosylated N-terminal proBNP in patients with heart failure (HF) and chronic renal failure (CRF) on hemodialysis (2011)
  • Author(s): T Nishikimi, M. Ikeda, Y Takeda, T Ishimitsu, N Minamino, K Kuwahara, H Kinoshita, Y Nakagawa, K Nakao
  • Organizer: ESC Congress 2011
  • Place of Presentation: Convention Center(Paris, France)
• [Presentation] Inhibition of HCN overexpression in failing heart of dilated cardiomyopathy mouse model by ivabradine prevents sudden arrhythmic death (2011)
  • Author(s): Y Kuwabara, K Kuwahara, H Kinoshita, M Takano, Y Nakagawa, S Usami, K Nakao, T Minami, Y Yamada, C Yamada, J Shibata, T Nishikimi1, K Ueshima and K Nakao
  • Organizer: American Heart Association Scientific Session 2011
  • Place of Presentation: Convention Center(Orlando, USA)
• [Presentation] N-Type Ca2+ Channel Blocker is Effective to Prevent Sudden Cardiac Death in Mice with Heart Failure (2011)
  • Author(s): Y Yamada, K Kuwahara, H Kinoshita, Y Kuwabara, Y Nakagawa, J Shibata, C Yamada, T Minami, S Yasuno, S Usami, K Nakao, T Nishikimi, K Ueshima,
  • Organizer: American Heart Association Scientific Session 2011
  • Place of Presentation: Convention Center(Orlando, USA)
• [Presentation] Induction of Myocardin-Related Transcription Factor-A Contributes to the Phenotypic Alteration of Vascular Smooth Muscle Cells and Plays a Pivotal Role in Vascular Remodeling (2011)
  • Author(s): T Minami, K Kuwahara, Y Nakagawa, H Kinoshita, K Nakao, Y Kuwabara, Y Yamada, C Yamada, J Shibata, S Usami, T Nishikimi, K Nakao
  • Organizer: American Heart Association Scientific Session 2011
  • Place of Presentation: Convention Center(Orlando, USA)
• [Presentation] ナトリウム利尿ペプチドBNP、ANPの最新知見とその臨床応用
  • Author(s): 錦見俊雄
  • Organizer: 日本自動分析学会
  • Place of Presentation: 横浜パシフィコ、横浜
  • Invited
• [Presentation] B型ナトリウム利尿ペプチド研究の最前線
  • Author(s): 錦見俊雄
  • Organizer: 日本高血圧学会総会
  • Place of Presentation: 大阪国際会議場、大阪
  • Invited
• [Presentation] O-glycosylated Pro B-type Natriuretic Peptide is Cleaved intracellularly By Furin in Ventricular Myocytes and Atrial Myocytes : Importance of the Distance Between the O-glycosylation Sites and Cleaved Sites
  • Author(s): Nishikimi Toshio 他12名
  • Organizer: American Heart Association
  • Place of Presentation: Dallas, USA
• [Presentation] 心不全におけるBNP前駆体 proBNPの病態生理的意義
  • Author(s): 錦見俊雄
  • Organizer: 日本心血管内分泌代謝学会
  • Place of Presentation: 千里ライフサイエンスセンター、大阪
  • Invited
• [Presentation] Molecular mechanism of lower processing rate in human proBNP: importance of the distance between the O-glycosylation and cleavage sites
  • Author(s): Nishikimi Toshio 他12名
  • Organizer: 日本循環器学会総会
  • Place of Presentation: 東京フォーラム
• [Patent(Industrial Property Rights)] proBNP-108の新規用途 (2013)
  • Inventor(s): 錦見俊雄、他1名
  • Industrial Property Rights Holder: 錦見俊雄、他2名
  • Acquisition Date: 2013-09-13
  • Overseas