| Project/Area Number |
23591052
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Kazuo 宮崎大学, 医学部, 教授 (50204912)
HATAKEYAMA Kinta 宮崎大学, 医学部, 准教授 (60325735)
NAGAMACHI Shigeki 宮崎大学, 医学部, 准教授 (40180517)
鬼塚 敏男 宮崎大学, 医学部, 教授 (60108595)
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| Co-Investigator(Renkei-kenkyūsha) |
ASADA Yujiro 宮崎大学, 医学部, 教授 (70202588)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 血管生物学 / マクロファージ / 解糖系 / 動脈瘤 / 動脈硬化 / 糖代謝 / 炎症 / 細胞外マトリックス |
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| Research Abstract |
Enhanced accumulation of the modified glucose analogue 18fluoro-deoxyglucose by positron emission tomography imaging in the human abdominal aortic aneurysm (AAA) was associated with protein expressions of glucose transporters-1 and -3, assessed by Western blot. The magnitude of glucose transporter-3 expression was correlated with matrix metalloproteinase (MMP)-9 activity, co-localized in macrophages of the aneurysmal wall. In monocytic cell line induced by phorbol 12-myristate 13-acetate, the inhibition of glucose transport or glycolysis abrogated the MMP-9 activity in these cells. Furthermore, the inhibition of glycolysis activity with the intra-peritoneal administration of 2-deoxyglucose significantly attenuated the dilatation of abdominal aorta induced by periaortic application of calcium chloride in C57BL/6J male mice or reduced the aneurysmal formation in angiotensin II-infused apolipoprotein E knockout male mice.
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