Research for the mechanism of aldosterone synthesis in the adrenal gland and the heart.
| Project/Area Number |
23591089
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Circulatory organs internal medicine
|
|---|
| Research Institution | Jikei University School of Medicine |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | アルドステロン / 心筋細胞 / 高血糖 / 虚血再灌流障害 / 鉱質コルチコイド受容体 / p38 MAPK / 循環器 / 病態生理 / 生理活性物質 |
|---|
| Research Abstract |
We aimed at the establishment of the experimental system which showed small quantity of aldosterone (Aldo) synthesized in the heart. Various conditions of culture medium and time course were examined using the cultured neonatal rat cardiomyocytes (NRCs). We found that CYP11B2 gene was expressed by short time stimulation of high glucose. This system will be extensively utilizable in the future research of cardiac Aldo synthesis. On the other hand, an unknown action of Aldo was examined by using the Langendorff system. It was found that the pre-treatment of Aldo attenuated ischemia reperfusion injury, suggesting that Aldo per se has a preconditioning action. Also, we newly established the cell culture system which showed the precise intracellular sodium concentration. By this system, we could measure the absolute increase in the intra-cellular sodium concentration stimulated by Aldo and cortisol. This system is also utilizable for the future studies of cardiac hypertrophy and others.
|
Report
(4 results)
Research Products
(18 results)
-
[Journal Article] Preconditioning actions of aldosterone through p38 signaling modulation in isolated rat hearts2014
Author(s)
Yoshino T, Nagoshi T, Anzawa R, Kashiwagi Y, Ito K, Katoh D, Fujisaki M, Kayama Y, Date Taro, Hongo K, Yoshimura M.
-
Journal Title
J Endocrinol
Volume: (in press)
Issue: 2
Pages: 289-299
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Biphasic action of aldosterone on Akt signaling in cardiomyocytes.2012
Author(s)
Nagoshi T, Date T, Fujisaki M, Yoshino T, Sekiyama H, Ogawa K, Kayama Y, Minai K, Komukai K, Ogawa T, Yoshimura M.
-
Journal Title
Horm Metab Res.
Volume: 44(13)
Issue: 13
Pages: 931-7
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Cardiac tamponade as an independent condition affecting the relationship between the plasma B-type natriuretic peptide levels and cardiac function.2012
Author(s)
Minai K, Komukai K, Arase S, Nagoshi T, Matsuo S, Ogawa K, Kayama Y, Inada K, Tanigawa SI, Takemoto T, Sekiyama H, Date T, Ogawa T, Taniguchi I, Yoshimura M.
-
Journal Title
Heart Vessels.
Volume: Epub ahead of print
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
