| Project/Area Number |
23591097
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
|
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| Research Institution | Chiba University |
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Principal Investigator |
TATENO Kaoru 千葉大学, 医学部附属病院, 助教 (20532758)
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| Co-Investigator(Renkei-kenkyūsha) |
MINAMINO Tohru 新潟大学, 医歯学総合研究科, 教授 (90328063)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 分子血管病態学 / 再生医療 / Notch |
|---|
| Research Abstract |
Peripheral blood mononuclear cell implantation is a promising method to treat critical limb ischemia. Nonetheless, 30% of the cases are non-responding. We found that Notch ligand expression in these patients was down regulated. Likewise, mice models deleting Notch ligands or receptors from donor cells or host ischemic muscles, respectively, revealed that Notch signaling components are critical in the mechanism of the therapy. We employed mice models for non-responding patients, i.e. diabetes or aged mice, and found that Notch signaling was attenuated following limb ischemia. Addition of Jagged-1, but not Dll-4, overexpressing cells to these models helped ischemia to heal more adequately. These results warrant us to further deploy a Notch targeting therapy for previously the non-responding patients.
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