| Project/Area Number |
23591105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kagoshima University |
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Principal Investigator |
MIYATA MASAAKI 鹿児島大学, 医歯(薬)学総合研究科, 准教授 (00347113)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUYAMA Takami 鹿児島大学, 医歯学総合研究科, 教授 (30145479)
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| Co-Investigator(Renkei-kenkyūsha) |
FURUSHO Yuko 鹿児島大学, 医歯学総合研究科, 助教 (00632514)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 動脈硬化 / 葉酸レセプターβ / マクロファージ / 活性化マクロファージ / 分子イメージング |
|---|
| Research Abstract |
The folic acid receptor beta (FR-beta) attracts attention as a marker of activated macrophages. We found that the activated macrophage which expressed FR-beta was present in the arteriosclerosis lesion of the arteriosclerosis model mouse. We administrated the recombinant immunotoxin which coupled Pseudomonas aeruginosa toxin to anti-FR-beta monoclonal antibody into the arteriosclerosis model mice, selectively killed the activated macrophages, and demonstrated that it reduced arteriosclerotic lesion.
In addition, we established a sandwich ELISA assay using anti-FR-beta monoclonal antibody and measured the soluble FR-beta concentration of human serum. The patients with aortic aneurysms showed a higher serum FR-beta level compared with the normal control. The measurement of the soluble FR-beta concentration is suggested to be useful for the diagnosis of atherosclerosis.
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