| Project/Area Number |
23591115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Keio University (2013)
Akita University (2012)
The University of Tokyo (2011) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAE Susumu 東京大学, 医科学研究所, 准教授 (60450409)
YAMANASHI Yuji 東京大学, 医科学研究所, 教授 (40202387)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 喘息 / チロシンキナーゼ / アダプター分子 / 疾患モデルマウス / 閉塞性肺疾患 / アダプタータンパク質 |
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| Research Abstract |
Dok adaptor proteins play important roles on the proper regulation of tyrosine kinase-mediated signaling pathways. In this study, I particularly investigated the role of Dok adaptors in pulmonary disorders such as asthma. Although many causative factors of asthma have been suggested, dysfunction of immune cells is one of the major reason of this disease. In these immune cells, three Dok adaptors can be detected, therefore, the role of them in asthma was investigated using knockout mice.
As expected, mice lacking three Dok adaptors exhibited an abnormal increase in airway hyperreactivity, a biological measure of asthma. Consistent with previous findings, the levels of Th2 cytokines in bronchoalveolar lavage fluids were also elevated in these mice. Furthermore, histological examination revealed hyperplasia of airway epithelial cells and accumulation of goblet cells. These results proved that Dok adaptor proteins play a key role on the regulation of asthma.
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