A role of p116Rip protein in the actin-myosin binding protein transgenic mouse on interstitial pneumonia

• Project/Area Number: 23591135
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Gunma University
• Principal Investigator: KOGA YASUHIKO 群馬大学, 医学(系)研究科(研究院), 助教 (10533862)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 非閉塞性肺疾患癌 / 肺線維症 / 呼吸器感染症 / ミオシン / p116Rip / 気道上皮 / リン酸化 / 間質性肺炎 / トランスジェニックマウス

Research Abstract

We examined the effect of specific siRNA against p116Rip on its protein expression level in human bronchial culture cells, HFL-1 ,A549 and HBEC-3 by western blot. The elimination of p116Rip protein expression was successfully performed using siRNA against p116Rip. Knocking down of p116Rip was accompanied with the elevation of myosin light chain phosphorylation in cells. Furthermore, we generated p116Rip-transgenic mice and examined the effect of p116Rip protein on the Bleomycin-induced acute lung injury model mice. However, p116Rip transgenic mice showed the minimal overexpression of exogenous p116Rip compared with wild type mouse, thus it is thought that we need to examine for further experiments to produce p116Rip transgenic mouse.

Research Products

• [Journal Article] Phase II study of oral S-1 plus cisplatin with bevacizumab for advanced non-squamous non-small cell lung cancer (2013)
  • Author(s): Kaira K, Tomizawa Y, Yoshino R, Miura Y, Yoshii A, Iwasaki Y, Koga Y, Ono A, Hisada T, Minato K, Sato K, Kazama T, Ishihara S, Kohyama K, Fueki N, Saito R, Sunaga N
  • Journal Title: Lung Cancer Volume: 13
  • Peer Reviewed
• [Journal Article] Phase II study of oral S-1 and cisplatin with concurrent radiotherapy for locally advanced non-small-cell lung cancer (2013)
  • Author(s): Kaira K, Tomizawa Y, Yoshino R, Yoshii A,Matsuura M, Iwasaki Y, Koga Y, Ono A, Nishioka M, Kamide Y, Hisada T, Ishizuka T, Shirai K, Ebara T, Saito J, Nakano T, Sunaga N
  • Journal Title: Lung Cancer Volume: 3 (Epub 2013 Sep 19) Pages: 449-54
  • Peer Reviewed
• [Journal Article] Phase II study of oral S-1 plus cisplatin with bevacizumab for advanced non-squamous non-small cell lung cancer. (2013)
  • Author(s): Kaira K, Tomizawa Y, Yoshino R, Miura Y, Yoshii A, Iwasaki Y, Koga Y, Ono A, Hisada T, Minato K, Sato K, Kazama T, Ishihara S, Kohyama K, Fueki N, Saito R, Sunaga N.
  • Journal Title: Lung Cancer Volume: 82(1) Pages: 103-8
  • Peer Reviewed
• [Journal Article] Phase II study of oral S-1 and cisplatin with concurrent radiotherapy for locally advanced non-small-cell lung cancer. (2013)
  • Author(s): Kaira K, Tomizawa Y, Yoshino R, Yoshii A, Matsuura M, Iwasaki Y, Koga Y, Ono A, Nishioka M, Kamide Y, Hisada T, Ishizuka T, Shirai K, Ebara T, Saitoh J, Nakano T, Sunaga N.
  • Journal Title: Lung Cancer Volume: 82(3) Pages: 449-54
  • Peer Reviewed
• [Journal Article] Extracellular acidification induces connective tissue growth factor production through proton-sensing receptor OGR1 in human airway smooth muscle cells (2011)
  • Author(s): Matsuzaki S, Ishizuka T, Yamada H, Kamide Y, Hisada T, Ichimonji I, Aoki H, Yatomi M, Komachi M, Tsurumaki H, Ono A, Koga Y, Dobashi K, Mogi C, Sato K, Tomura H, Mori M, Okajima F
  • Journal Title: Biochem Biophys Res Commun Volume: 413(4) (Epub 2011 Sep 1) Pages: 499-503
  • Peer Reviewed
• [Journal Article] Intracellular glutathione redox status in human dendritic cells regulates IL-27 production and T-cell polarization (2011)
  • Author(s): Kamide Y, Utsugi M, Dobashi K, Ono A, Ishizuka T, Hisada T, Koga Y, Uno K, Hamuro J, Mori M
  • Journal Title: Allergy Volume: 66(9) Pages: 1183-92
  • Peer Reviewed
• [Journal Article] A young adult case of poorly differentiated lung adenocarcinoma showing the resistance mutation T790M of EGFR before treatment with tyrosine kinase inhibitors (2011)
  • Author(s): Okamura T, Shibusawa N, Koga Y, Horiguchi N, Ogashiwa T, Satoh K, Hisada T, Satoh T, Ishizuka M, Mori M.m
  • Journal Title: Nihon Naika Gakkai Zasshi Volume: 100(7) Pages: 1959-62
  • NAID: 10029748006
  • Peer Reviewed
• [Journal Article] Extracellular acidification induces connective tissue growth factor production through proton-sensing receptor OGR1 in human airway smooth muscle cells. (2011)
  • Author(s): Matsuzaki S, Ishizuka T, Yamada H, Kamide Y, Hisada T, Ichimonji I, Aoki H, Yatomi M, Komachi M, Tsurumaki H, Ono A, Koga Y, Dobashi K, Mogi C, Sato K, Tomura H, Mori M, Okajima F.
  • Journal Title: Biochem Biophys Res Commun. Volume: 413(4) Pages: 499-503
  • Peer Reviewed
• [Journal Article] Intracellular glutathione redox status in human dendritic cells regulates IL-27 production and T-cell polarization. (2011)
  • Author(s): Kamide Y, Utsugi M, Dobashi K, Ono A, Ishizuka T, Hisada T, Koga Y, Uno K, Hamuro J, Mori M.
  • Journal Title: Allergy Volume: 66(9) Pages: 1183-92
  • Peer Reviewed
• [Journal Article] A young adult case of poorly differentiated lung adenocarcinoma showing the resistance mutation T790M of EGFR before treatment with tyrosine kinase inhibitors. (2011)
  • Author(s): Okamura T, Shibusawa N, Koga Y, Horiguchi N, Ogashiwa T, Satoh K, Hisada T, Satoh T, Ishizuka M, Mori
  • Journal Title: Nihon Naika Gakkai Zasshi Volume: 100(7) Pages: 1959-62
  • NAID: 10029748006
  • Peer Reviewed
• [Presentation] p116Rip proteinの培養細胞でのgene silencingの影響について (2013)
  • Author(s): 古賀康彦
  • Organizer: 第63回日本アレルギー学会
  • Place of Presentation: ホテルオークラ(東京都)
  • Year and Date: 2013-11-30
• [Presentation] 維持透析下に5th lineまでの化学療法を行った進展型小細胞肺癌の長期生存が得られた一例 (2013)
  • Author(s): 古賀康彦
  • Organizer: 第11回臨床腫瘍学会
  • Place of Presentation: 仙台国際センター(仙台市)
  • Year and Date: 2013-08-30
• [Presentation] 気道培養細胞におけるp116Rip gene silencingについての検討 (2013)
  • Author(s): 古賀康彦
  • Organizer: 第53回日本呼吸器学会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2013-04-21
• [Presentation] p116Rip proteinの気道培養細胞でのサイトカイン分泌に及ぼす影響 (2013)
  • Author(s): 古賀 康彦
  • Organizer: 日本呼吸器学会
  • Place of Presentation: 東京国際フォーラム、有楽町、東京
• [Presentation] p116Rip proteinの培養細胞でのgene silencingの影響について (2013)
  • Author(s): 古賀 康彦
  • Organizer: 第６３回 日本アレルギー学会
  • Place of Presentation: ホテル ニューオータニ、東京
• [Presentation] 維持透析下に5th lineまでの化学療法を行った進展型小細胞肺癌の長期生存が得られた一例 (2013)
  • Author(s): 古賀 康彦
  • Organizer: 第11回日本臨床腫瘍学会
  • Place of Presentation: 仙台国際センター、仙台、宮城
• [Presentation] 気道線維芽細胞におけるMLCP結合蛋白p116Ripの発現の検討 (2011)
  • Author(s): 古賀康彦
  • Organizer: 第51回日本呼吸器学会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2011-04-23