The role of proteoglycans in the modulation of EGFR family and it's ligand in lung fibrosis

• Project/Area Number: 23591139
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: JO Taisuke 東京大学, 保健・健康推進本部, 助教 (30376470)
• Co-Investigator(Kenkyū-buntansha): YAMAUCHI Yasuhiro 東京大学, 保健・健康推進本部, 助教 (00323585) ; SAITO Akira 東京大学, 保健・健康推進本部, 助教 (90591412)
• Co-Investigator(Renkei-kenkyūsha): NAGASE Takaide 東京大学, 医学部附属病院, 教授 (40208004) ; KOHYAMA Tadashi 帝京大学, 医学部, 教授 (00302703)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 肺線維症 / プロテオグリカン / EGFRファミリー / マウス / ブレオマイシン / EGFR

Research Abstract

Idiopathic Pulmonary Fibrosis is a progressive disease with poor clinical outcome. And yet treatment strategy has not been well-established. In this study we examined the expression of Epidermal Growth Factor Receptors (EGFR) and it`s ligands in isolated lungs from mouse model of bleomycin-induced pulmonary fibrosis. We compared C56BL6 and Balb/c, a strain known to show stronger fibrotic change and mild fibrotic change with bleomycin, respectively. Proteoglycan expression was concomitantly evaluated with intention to explore the association with EGFR and EGFR ligands. Lungs from C56BL6 mice appeared to have apparent fibrotic changes and higher collagen content compared to Balb/c mice. Expression of amphiregulin, epiregulin, versican and decorin seemed to be lower in C56BL6 compared Balb/c mice, whilst EGFR seemed to be highly expressed in C56BL6. These findings provide bases for further investigation.

Research Products

• [Journal Article] Histamine induces human lung fibroblast-mediated collagen gel contraction via histamine H1 receptor. (2014)
  • Author(s): 5.Horie M, Saito A, Yamauchi Y, Mikami Y, Sakamoto M, Jo T, Nakajima J, Takizawa H, Nagase T, Kohyama T.
  • Journal Title: Exp Lung Res. Volume: 40 Issue: 5 Pages: 222-36
  • DOI: 10.3109/01902148.2014.900155
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Histamine induces human lung fibroblast-mediated collagen gel contraction via histamine H1 receptor (2014)
  • Author(s): Horie M, Saito A, Yamauchi Y, Mikami Y, Sakamoto M, Jo T, Takizawa H, Nagase T, Kohyama T
  • Journal Title: Experimental Lung Research Volume: 未確定
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor superfamily member LIGHT induces epithelial-mesenchymal transition in A549 human alveolar epithelial cells. (2012)
  • Author(s): Mikami Y
  • Journal Title: Biochem Biophys Res Commun. Volume: 428 Issue: 4 Pages: 451-7
  • DOI: 10.1016/j.bbrc.2012.10.097
  • Peer Reviewed
• [Presentation] Interleukin-17A Enhances Interferon- Gamma-Induced CXCL10/IP-10 Production In BEAS-2B Human Bronchial Epithelial Cells (2013)
  • Author(s): Yasuhiro Yamauchi, Taisuke Jo, Masafumi Horie, Yu Mikami, Satoshi Noguchi, Osamu Narumoto, Kazutaka Takami, Tadashi Kohyama, Hajime Takizawa, Takahide Nagase
  • Organizer: American Thoracic Society International Conference
  • Place of Presentation: Pennsylvania Convention Center
• [Presentation] Tumor Necrosis Factor Super Family Member LIGHT Induces CXCL8 Production In BEAS-2B Human Bronchial Epithelial Cells Via Erk1/2 Pathway (2013)
  • Author(s): Yu Mikami, Yasuhiro Yamauchi, Masafumi Horie, Satoshi Noguchi, Osamu Narumoto, Taisuke Jo, Tadashi Kohyama, Hajime Takizawa, Takahide Nagase
  • Organizer: American Thoracic Society International Conference
  • Place of Presentation: Pennsylvania Convention Center
• [Presentation] Interleukin-17A Enhances Interferon-Gamma-Induced CXCL10/IP-10 Production In BEAS-2B Human Bronchial Epithelial Cells. (2013)
  • Author(s): Yasuhiro Yamauchi, Taisuke Jo, Masafumi Horie, Yu Mikami, Satoshi Noguchi, Osamu Narumoto, Kazutaka Takami, Tadashi Kohyama, Hajime Takizawa, Takahide Nagase,
  • Organizer: American Thoracic Society International Conference 2013
  • Place of Presentation: Pennsylvania convention center (アメリカ、フィラデルフィア）
• [Presentation] Tumor Necrosis Factor Super Family Member LIGHT Induces CXCL8 Production In BEAS-2B Human Bronchial Epithelial Cells Via Erk1/2 Pathway. (2013)
  • Author(s): Yu Mikami, Yasuhiro Yamauchi, Masafumi Horie, Satoshi Noguchi, Osamu Narumoto, Taisuke Jo, Tadashi Kohyama, Hajime Takizawa, Takahide Nagase
  • Organizer: American Thoracic Society International Conference 2013
  • Place of Presentation: Pennsylvania convention center (アメリカ、フィラデルフィア）