The role of cancer associated fibroblast in non small cell lung cancer

• Project/Area Number: 23591161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Juntendo University
• Principal Investigator: TOGO Shinsaku 順天堂大学, 医学部, 助教 (80365634)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 癌関連線維芽細胞 / 非小細胞肺癌 / EMT / 癌微小環境

Research Abstract

The activated fibroblasts that are found in association with cancer cells are known as cancer-associated fibroblasts (CAFs). CAFs probably promote tumor progression through specific communications with cancer cells. Methods: Control: derived from normal lung tissue and CAFs: derived from tumor in same donors were prepared from surgical tissues. The ability of fibroblasts to mediate contraction of three dimensional type I collagen gels and Fibroblasts chemotaxis were evaluated. Fibroblasts cell viability by chemoreagents was assayed. Primary fibroblasts preserved their ability to induce migration and collagen gel contraction, which was greater with CAFs than NFs and the condition media from CAFs stimulated more A549 chemotaxis. The cell viability of CAFs against CDDP showed higher than that of controls. These results showed that CAFs can maintain the phenotypic properties of myofibroblasts and be further activated the myofibroblast activity interaction with carcinoma cells.

Research Products

• [Journal Article] Carcinoma-Associated Fibroblasts Are a Promising Therapeutic Target (2013)
  • Author(s): Togo, S.; Polanska, U.M.; Horimoto, Y.; Orimo, A
  • Journal Title: Cancers Volume: 5 Pages: 149-169
• [Journal Article] Carcinoma-associated fibroblasts are a promising therapeutic target. (2013)
  • Author(s): 1. Togo S, Polanska UM, Horimoto Y, Orimo A
  • Journal Title: cancers Volume: 31 Issue: 1 Pages: 149-69
  • DOI: 10.3390/cancers5010149
  • Peer Reviewed
• [Journal Article] Carcinoma-Associated Fibroblasts are a promising therapeuic target. Review (2013)
  • Author(s): S.Togo, Orimo. A et al
  • Journal Title: Cancers Volume: 5 Pages: 149-169
  • Peer Reviewed
• [Presentation] Stromal fibroblasts associated with cancer cells enhanced trans differentiation in myofibroblast (2012)
  • Author(s): Togo S
  • Organizer: American Thoracic Society
  • Place of Presentation: San Francisco
  • Year and Date: 2012-05-23
• [Presentation] 非小細胞肺癌間質内における筋線維芽細胞の機能解析 (2011)
  • Author(s): 長浜久美、十合晋作
  • Organizer: 第51回日本呼吸器学会学術集会
  • Place of Presentation: 東京
• [Presentation] 非小細胞肺癌間質内における筋線維芽細胞の機能解析の検討 (2011)
  • Author(s): 十合晋作
  • Organizer: 第52回日本肺癌学会総会
  • Place of Presentation: 大阪
• [Presentation] 非小細胞肺癌間質内における筋線維芽細胞の機能解析 (2011)
  • Author(s): 十合 晋作
  • Organizer: 第52回日本肺癌学会学術講演会
  • Place of Presentation: 大阪
• [Presentation] 非小細胞肺癌間質内における筋線維芽細胞の機能解析の検討 (2011)
  • Author(s): 十合 晋作
  • Organizer: 第72回日本癌学会学術講演会
  • Place of Presentation: 名古屋
• [Book] 肺癌と癌関連線維芽細胞 (2012)
  • Author(s): 十合晋作、高橋和久
  • Publisher: 中外医学社Annual Review呼吸器