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Patent development and clinical application of new diagnostic methods of tuberculosis infection

Research Project

Project/Area Number 23591168
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionKawasaki Medical School

Principal Investigator

KOBASHI YOSHIHIRO  川崎医科大学, 医学部, 准教授 (90205443)

Co-Investigator(Kenkyū-buntansha) KATO Shigeki  川崎医科大学, 医学部, 講師 (10295206)
MOURI Keiji  川崎医科大学, 医学部, 講師 (20341092)
ABE Masaaki  川崎医科大学, 医学部, 臨床助教 (50509221)
IKEDA Masaki  川崎医科大学, 医学部, 臨床助教 (90551135)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords結核感染診断法 / クオンティフェロン / T-SPOT.TB / サイトカイン / ケモカイン / 局所検体 / QuantiFERON / IGRA
Outline of Final Research Achievements

The challenge of development of useful new diagnostic methods for tuberculosis infection.
1)In order to increse the sensitivity and specificity, we measured the colony forming unit of IFN-γ produced by the stimulation of Mycobacterium tuberculosis (MTB)-specific antigen after the isolation of monocytes using the clinical specimens from the inflammation sites. As a result, when we used pleural effusion, highest sensitivity was obtained comparing to other diagnostic methods.
2)We measured several cytokines and chemokinesin the supernatant stimulated by MTB-specific antigens. IP-10 and MIG were useful to differentiate active TB disease and other diseases as well as IFN-γ. On the other hand, IL-2 showed most poor results. If the new diagnostic kit which can be measured IP-10 and IL-2 with IFN-γ simultaneously was developed, it may be possible to discriminate active TB disease and latent TB disease or healed TB disease in future.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (43 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (25 results) (of which Peer Reviewed: 23 results,  Open Access: 5 results) Presentation (18 results) (of which Invited: 3 results)

  • [Journal Article] M.abscessus complexによる呼吸器感染症2015

    • Author(s)
      小橋吉博
    • Journal Title

      呼吸器内科

      Volume: 27(1) Pages: 54-57

    • Related Report
      2014 Annual Research Report
    • Open Access
  • [Journal Article] Clinical applicationof T-SPOT.TB using pleural effusion as a diagnostic method for tuberculosis infection2014

    • Author(s)
      Kobashi Y, Mouri K, Obase Y, Kato S, Oka M
    • Journal Title

      Open Journal of Respiratory Diseases

      Volume: 4 Pages: 64-72

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mycobacterium avium genotype is assosiated with therapeutic response to lung infection.2014

    • Author(s)
      Kikuchi T, Kobashi Y, Hirano T, Tode N, Santoso A, Tamada T, Fujimura S, Mitsuhashi Y, Honda Y, Nukiwa T, Kaku M, Watanabe A, Ichinose M.
    • Journal Title

      Clinical Microbiology and Infection

      Volume: 20(3) Pages: 256-262

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased level of plasma galectin-9 in patients with influenza virus infection2014

    • Author(s)
      Kato S, Ikeda M, Schimizu H, Mouri K, Obase Y, Kobashi Y, Fukushima K, Hiramatsu M, Oka M.
    • Journal Title

      The Tohoku Journal of Experimental Medicine

      Volume: 232(4) Pages: 263-267

    • NAID

      130004822407

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 結核菌特異的インターフェロンーγ遊離試験クオンティフェロン(QFT)検査の実施状況と結果解析2014

    • Author(s)
      上杉里枝、河口勝憲、黒川幸徳、通山 薫、小橋吉博
    • Journal Title

      医学検査

      Volume: 63(4) Pages: 497-503

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical application of T-SPOT.TB using pleural effusion as a diagnostic method for tuberculosis infection2014

    • Author(s)
      Kobashi Y, Mouri K, Obase Y, Kato S, Oka M
    • Journal Title

      Open Journal of Respiratory Diseases

      Volume: 4 Pages: 64-72

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mucobacterium avium genotype is associated with the therapeutic response to lung infection.2014

    • Author(s)
      Kikuchi T, Kobashi Y, Hirano T, Tode N, Santoso A, Tamada T, Fujimura S, Mitsuhashi Y, Honda Y, Nukiwa T, Kaku M, Watanabe A, Ichinose M
    • Journal Title

      Clinical Microbiology and Infection

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Increased levels of plasma galectin-9 in patients with influenza virus infection.2014

    • Author(s)
      Kato S, Ikeda M, Shimizu H, Mouri K, Obase Y, Kobashi Y, Fukushima K, Hirashima M, Oka M.
    • Journal Title

      Tohoku Journal of Experimental Medicine

      Volume: 4 Pages: 263-267

    • NAID

      130004822407

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Serological assay by use of glycopeptidolipid core antigen for Mycobacterium avium complex.2013

    • Author(s)
      Kobashi Y, Mouri K, Obase Y, Kato S, Oka M
    • Journal Title

      Scandinavian Journal of Infectious Disease

      Volume: 45 Pages: 241-249

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical analysis of patients with pulmonary nontuberculous mycobacterial disease complicated by pneumothorax2013

    • Author(s)
      Kobashi Y, Mouri K, Obse Y, Kato S, Oka M.
    • Journal Title

      Internal Medicine

      Volume: 52 Pages: 2511-2515

    • NAID

      130003383696

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mucoid impaction of the bronchi caused by Mycobacterium avium.2013

    • Author(s)
      Kobashi Y, Mouri K, Obase Y, Kato S, Nakata M, Oka M.
    • Journal Title

      Internal Medicine

      Volume: 52 Pages: 1537-1540

    • NAID

      130003365685

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical analysis of pulmonary nontuberculous mycobacterial disease diagnosed as coincidental pulmonary infection due to Mycobacterium sp.2013

    • Author(s)
      Kobashi Y, Mouri K, Obase Y, Kato S, Oka M.
    • Journal Title

      Open Journal of Respiratory Diseases

      Volume: 3 Pages: 107-112

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] T-SPOTの臨床応用-特にT-SPOT.TBについて2013

    • Author(s)
      小橋吉博
    • Journal Title

      呼吸

      Volume: 32 Pages: 723-727

    • Related Report
      2013 Research-status Report
  • [Journal Article] Mucoid impaction of the bronchi caused by Mycobacterium avium2013

    • Author(s)
      Kobashi Y, MOuri K, Obase Y, Kato S, Nakata M, Oka M.
    • Journal Title

      Internal Medicine

      Volume: in press

    • NAID

      130003365685

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical analysis of pulmonary nontuberculous mycobacterial disease diagnosed as coincidental pulmonary infection due to Mycobacterium sp.2013

    • Author(s)
      Kobashi Y, Mouri K, Obase Y, Kato S, Oka M.
    • Journal Title

      Open Journal of Respiratory Disease

      Volume: in press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mycobacterium kyorinense infection2013

    • Author(s)
      Ohnishi H, Yonetani S, Wada H, Takeshita K, Kuramochi D, Mikura S, Narisawa E, Fujita A, FunayamaY, Kobashi Y, Sakakibara Y, Ishiyama Y, Takakura S, Goto H, Watanabe T, et al.
    • Journal Title

      Emerging infectious diseasese

      Volume: 19(3) Pages: 508-510

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationship between clinical efficacy for pulmonary MAC and drug-sensitivity test for isolated MAC in a recent 6-year period.2012

    • Author(s)
      Kobashi Y, Mouri K, Obase Y, Kato S, Oka M.
    • Journal Title

      Journal of Infection and Chemotherapy

      Volume: 18(4) Issue: 4 Pages: 436-443

    • DOI

      10.3109/00365548.2012.714904

    • NAID

      10030947814

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pulmonary Mycobacterium kyorinense disease showed clinical improvement following combined therapy with clarithromycin and levofloxacin.2012

    • Author(s)
      Kobashi Y, Mouri K, Obase Y, Kato S, Oka M.
    • Journal Title

      Internal Medicine

      Volume: 51(14) Pages: 1923-1926

    • NAID

      130002062225

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Usefulness of tuberculin skin test and three interferon-gamma release assays for the differential diagnosis of pulmonary tuberculosis.2012

    • Author(s)
      Kobashi Y, Abe M, Mouri K, Obase Y, Miyashita N, Oka M.
    • Journal Title

      Internal Medicine

      Volume: 51(10) Pages: 1199-1205

    • NAID

      130002062440

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical and microbiological differences between Mycobacterium abscessus and Mycobacterium massiliense lung diseases.2012

    • Author(s)
      Harada T, Akiyama Y, Kurashima A, Nagai H, Tsuyuguchi K, Fujii T, Yano S, Shigeto E, Kuraika T, Kajiki A, Kobashi Y, Kokubu F, Sato A, Yoshida S, Iwamoto T, Sito H.
    • Journal Title

      Journal of Clinical Microbiology

      Volume: 50(11) Issue: 11 Pages: 3556-3561

    • DOI

      10.1128/jcm.01175-12

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Usefulness of tuberculin skin test and three interferon-gamma release assays for the differential diagnosis of pulmonary tuberculosis.2012

    • Author(s)
      Yoshihiro Kobashi
    • Journal Title

      Internal Medicine

      Volume: in press

    • NAID

      130002062440

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pulmonary Mycobacterium kyorinense disease showed improvement following combined therapy with clarithromycin and levofloxacin.2012

    • Author(s)
      Yoshihiro Kobashi
    • Journal Title

      Internal Medicine

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationship between clinical efficacy for pulmonary MAC disease and drug-sensitivity test for isolated MAC in a recent 6-year period.2012

    • Author(s)
      Yoshihiro Kobashi
    • Journal Title

      Iournal of Infection and Chemotherapy

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 局所検体を用いてT-SPOT.TBによる結核感染診断の臨床評価2011

    • Author(s)
      小橋吉博
    • Journal Title

      結核

      Volume: 12 Pages: 994-998

    • NAID

      10030264307

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pulmonary Mycobacterium massiliense disease with septicemia during immunosuppressive treatment.2011

    • Author(s)
      Yoshihiro Kobashi
    • Journal Title

      Internal Medicine

      Volume: 50 Pages: 1069-1073

    • NAID

      130000650116

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] IGRAを含む結核感染診断に対する新たな検査法の臨床評価2015

    • Author(s)
      小橋吉博
    • Organizer
      第55回日本呼吸器学会学術講演会
    • Place of Presentation
      東京国際フォーラム(東京)
    • Year and Date
      2015-04-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] IGRAsの臨床的有用性と解釈上の問題点:結核の活動性を評価できるか?2015

    • Author(s)
      小橋吉博
    • Organizer
      第90回日本結核病学会総会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 結核感染診断の現状と将来ー最近の知見を含めて2014

    • Author(s)
      小橋吉博
    • Organizer
      第84回日本感染症学会西日本地方会学術集会
    • Place of Presentation
      岡山ママカリフォーラム(岡山)
    • Year and Date
      2014-10-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Clinical evaluation of MIGRAs (IP-10 and <IG) including two IGRAs (QFT and T-SPOT) for the diagnosis of tuberculosis infection2014

    • Author(s)
      Kobashi Y
    • Organizer
      European Respiratory Society Annual Congress 2014
    • Place of Presentation
      ICM-Messe Munchen (Munich, Germany)
    • Year and Date
      2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 抗酸菌感染症2014

    • Author(s)
      小橋吉博
    • Organizer
      第51回日本呼吸器学会中国四国地方会
    • Place of Presentation
      米子コンベンションセンター(米子)
    • Year and Date
      2014-07-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] IGRAを含めた結核感染診断に対する新たな検査法の臨床評価2014

    • Author(s)
      小橋吉博
    • Organizer
      第88回日本感染症学会総会
    • Place of Presentation
      ヒルトン福岡シーホーク(福岡)
    • Year and Date
      2014-06-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 局所検体を用いたT-SPOT.TBによる結核感染診断の臨床評価2011

    • Author(s)
      小橋吉博
    • Organizer
      第86回日本結核病学会総会(招待講演)
    • Place of Presentation
      日本教育会館(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 体腔液を用いたIGRAによる結核感染診断への臨床応用2011

    • Author(s)
      小橋吉博
    • Organizer
      第109回日本内科学会講演会
    • Place of Presentation
      みやこめっせ(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 非結核性抗酸菌症の診断におけるIGRAの臨床評価2011

    • Author(s)
      小橋吉博
    • Organizer
      第52回日本呼吸器学会総会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 肺結核の鑑別診断におけるツ反と3つのIGRAの有用性について2011

    • Author(s)
      小橋吉博
    • Organizer
      第52回日本呼吸器学会総会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Comparative study of the usefulness of TST and three interferon-gamma release assays (IGRAs) for the differential diagnosis of pulmonary tuberculosis.2011

    • Author(s)
      Yoshihiro Kobashi
    • Organizer
      Annual Congress of European Respiratory Society
    • Place of Presentation
      Amsterdam RAI center (Amsterdam, Netherland)
    • Related Report
      2011 Research-status Report
  • [Presentation] 結核感染診断におけるQFT抗原刺激上清中におけるIP-10およびMCP-2の評価-QFTおよびT-SPOT.TBとの比較検討

    • Author(s)
      小橋吉博
    • Organizer
      第53回日本呼吸器学会学術講演会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] 結核感染診断におけるQFT抗原刺激上清中におけるIP-10およびMIGの評価ーQFTおよびT-SPOT.TBとの比較検討

    • Author(s)
      小橋吉博
    • Organizer
      第87回日本感染症学会総会
    • Place of Presentation
      横浜国際会議場(神奈川県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 結核感染診断におけるQFT抗原刺激上清中におけるIP-10およびMCP-2の評価-QFTおよびT-SPOT.TBとの比較検討

    • Author(s)
      小橋吉博
    • Organizer
      第53回日本呼吸器学会学術講演会
    • Place of Presentation
      東京国際フォーラム(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] 結核感染診断に対するQFT抗原刺激上清中におけるI-10およびMIGの評価-QFTおよびT-SPOT.TBとの比較検討

    • Author(s)
      小橋吉博
    • Organizer
      第87回日本感染症学会総会
    • Place of Presentation
      横浜国際会議場(神奈川県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 非結核性抗酸菌症の診断におけるIGRA(interferon-gamma release assay)の臨床評価

    • Author(s)
      小橋吉博
    • Organizer
      第52回日本呼吸器学会学術講演会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 肺結核の鑑別診断におけるツ反と3つのIGRAの有用性について

    • Author(s)
      小橋吉博
    • Organizer
      第52回日本呼吸器学会学術講演会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 体腔液を用いたIGRAによる結核感染診断への臨床応用

    • Author(s)
      小橋吉博
    • Organizer
      第109回日本内科学会講演会
    • Place of Presentation
      みやこメッセ(京都府)
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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