Expression and function of peptide transporter in the lung cancer

• Project/Area Number: 23591174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Nagoya City University (2013-2014); The Tazuke Kofukai (2011-2012)
• Principal Investigator: TAKEMURA MASAYA 名古屋市立大学, 医学(系)研究科(研究院), 助教 (30378707)
• Co-Investigator(Kenkyū-buntansha): FUKUI Motonari 財団法人田附興風会・医学研究所, 第2研究部, 部長 (50342697) ; YUBA Yoshiaki 財団法人田附興風会・医学研究所, 第4研究部, 研究主幹 (90465948)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 肺癌 / ペプチドトランスポーター / PEPT

Outline of Final Research Achievements

Lung cancer tissue and normal bronchial tissue were obtained from surgically resected lung specimens of 29 patients with lung cancer (19 male, mean age 68.8 years, 21 adenocarcinoma and 8 squamous carcinoma). Uptake experiments of the fluorescent D-Ala-Lys-AMCA which is identified as peptide transporter substrate for peptide transporter (PEPT2) showed higher tendency of accumulated fluorescence intensity in the squamous cell carcinoma tissue compaired to adenocarcinoma (p=0.09). Positive staining of immunohistochemistry for PEPT2 showed in both cancer tissues and normal bronchial tissues. Quantitatively, mRNA levels of PEPT2 were significantly higher in the squamous cell carcinoma tissues compaired to adenocarcinoma (p=0.038).