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Analysis of activation of glomerular parietal epithelial cells to podocyte injury

Research Project

Project/Area Number 23591176
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionHokkaido University

Principal Investigator

SASAKI Satoshi  北海道大学, 医学(系)研究科(研究院), 客員研究員 (70312345)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords糸球体硬化症 / 慢性腎疾患 / 糸球体上皮細胞 / 腎臓学 / ネフローゼ症候群 / 巣状分節性糸球体硬化症
Research Abstract

Recent investigations have disclosed that activation of parietal epithelial cells (PECs) plays key roles in the process of focal segmental glomerulosclerosis (FSGS). In this experimental project, we have tried to investigate dynamic behavior of activated PECs in the evolution of FSGS. We conducted serial assessment of activated PECs using the mouse models of lipopolysaccharide(LPS)-induced proteinuria and adriamycin (ADR) nephrosis. To further analyze the role of activated PECs, we induced rapid progressive podocytopathy using LPS-treated ADR nephrosis mice. As the cell markers for activation, we utilized two markers , CD44 and CXCR4. Using models of ADR nephrosis in mice of different ages, we found that CD44+PECs play significant roles in progressive glomerulosclerosis and the prevalence of the cells reflects different degrees of podocyte injury. In addition, analysis of two different markers for PEC activation clarified different roles of these markers during cell activation process.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2013 2012 2011

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results)

  • [Journal Article] Prevalence of CD44-Positive Glomerular Parietal Epithelial Cells Reflects Podocyte Injury in Adriamycin Nephropathy2013

    • Author(s)
      Takayuki Okamoto, Satoshi Sasaki, Takeshi Yamazaki, Yasuyuki Sato, Hironobu Ito, Tadashi Ariga
    • Journal Title

      Nephron Exp Nephrol

      Volume: 124 Issue: 3-4 Pages: 11-18

    • DOI

      10.1159/000357356

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two-year outcome of the ISKDC regimen and frequent-relapsing risk in children with idiopathic nephrotic syndrome2013

    • Author(s)
      Koichi Nakanishi、Kazumoto Iijima、Kenji Ishikura、Satoshi Sasak、Masataka Honda、Norio Yosikawa
    • Journal Title

      Clin J Am Soc Nephrol

      Volume: Vol.8 Issue: 5 Pages: 756-762

    • DOI

      10.2215/cjn.09010912

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prospective 5-year follow-up of cyclosporine treatment in children with steroid-resistant nephrosis2013

    • Author(s)
      Yuko Hamasaki、Norio Yoshikawa、Satoshi Sasaki、Kazumoto Iijima、Koichi Nakanishi、Shuichi Ito、Masataka Honda
    • Journal Title

      Pediatr Nephrol

      Volume: Vol.28 Pages: 765-771

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Podocyte expression of nonmuscle myosin heavy chain-IIA decreases in idiopathic nephrotic syndrome, especially in focal segmental glomerulosclerosis.2013

    • Author(s)
      Miura K, Kurihara H, Horita S, Chikamoto H, Hattori M, Harita Y, Tsurumi H, Kajiho Y, Sawada Y, Sasaki S, Igarashi T, Kunishima S, Sekine T.
    • Journal Title

      Nephrol Dial Transplant.

      Volume: 28 Issue: 12 Pages: 2993-3003

    • DOI

      10.1093/ndt/gft350

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] for the Japanese Study Group of Renal Disease in Children. Two-Year Follow-Up of a Prospective Clinical Trial of Cyclosporine for Frequently Relapsing Nephrotic Syndrome in Children.2012

    • Author(s)
      Ishikura K, Yoshikawa N, Nakazato H, Sasaki S, Iijima K, Nakanishi K, Matsuyama T, Ito S, Yata N, Ando T, Honda M
    • Journal Title

      Clin J Am Soc Nephrol

      Volume: 7 Issue: 10 Pages: 1576-1583

    • DOI

      10.2215/cjn.00110112

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A patient with Dent disease and features of Bartter syndrome caused by a novel mutation of CLCN52012

    • Author(s)
      Takayuki Okamoto、Toshihiro Tajima、Hirayama T、Satoshi Sasaki
    • Journal Title

      Eur J Pediatr

      Volume: Vol.171 Issue: 2 Pages: 401-404

    • DOI

      10.1007/s00431-011-1578-3

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Serial assessment of activated parietal epithelial cells in the LPS-induced proteinuria and adriamycin nephrosis2013

    • Author(s)
      Takeshi Yamazaki、Satoshi Sasaki、Asako Hayashi、Yasuyuki Sato、Takayuki Okamoto、Tadashi Ariga
    • Organizer
      World Congress of Nephrology
    • Place of Presentation
      Hong Kong、China
    • Year and Date
      2013-06-01
    • Related Report
      2013 Final Research Report
  • [Presentation] アドリアマイシン腎症におけるポドサイト喪失に伴う活性化壁側上皮細胞の意義2013

    • Author(s)
      山崎健史、佐々木聡、林麻子、佐藤泰征、岡本孝之、有賀正
    • Organizer
      第56回日本腎臓学会学術総会
    • Place of Presentation
      東京
    • Year and Date
      2013-05-12
    • Related Report
      2013 Final Research Report
  • [Presentation] アドリアマイシン腎症におけるポドサイト喪失に伴う活性化壁側上皮細胞の動態2013

    • Author(s)
      山崎健史, 佐々木聡, 林麻子, 佐藤泰征, 岡本孝 之, 有賀正
    • Organizer
      第56回日本腎臓学会学術総会
    • Place of Presentation
      東京国際フォーラム(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Serial assessment of activated parietal epithelial cells in the mouse model of LPS-induced proteinuria and adriamycin nephrosis2013

    • Author(s)
      Takeshi Yamazaki, Satoshi Sasaki, Asako Hayashi, Yasuyuki Sato, Takayuki Okamoto, Tadashi Ariga
    • Organizer
      World Congress of Nephrology 2013
    • Place of Presentation
      Hong Kong convention center (Hong Kong, China)
    • Related Report
      2013 Annual Research Report 2012 Research-status Report
  • [Presentation] アドリアマイシン腎症におけるポドサイト喪失に伴う活性化壁側上皮細胞の動態2013

    • Author(s)
      山崎健史、佐々木聡、林麻子、佐藤泰征、岡本孝之、有賀正
    • Organizer
      第56回日本腎臓学会学術総会
    • Place of Presentation
      東京国際フォーラム (東京)
    • Related Report
      2012 Research-status Report
  • [Presentation] Immunosuppressive therapy in childhood nephrotic syndrome-A look into the future-2011

    • Author(s)
      Sasaki S
    • Organizer
      The 11th Asian Congress of Pediatric Nephrology 2011
    • Place of Presentation
      福岡市, Fukuoka International Congress Center
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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