An analysis of urinary microRNAs and proteomes in renal diseases.

• Project/Area Number: 23591178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Yamagata University
• Principal Investigator: KONTA Tsuneo 山形大学, 医学部, 准教授 (60333952)
• Co-Investigator(Renkei-kenkyūsha): MASHIMA Yusuke 山形大学, 医学部, 医員 (60596576)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: microRNA / 尿 / 腎臓病

Research Abstract

We examined the profiles of urinary microRNAs (UmiRNAs) in 5 healthy controls and 71 patients with various renal diseases. Urinary concentration of miRNAs increased in patients with renal disease compared with healthy controls. The microarray analysis detected 83-137 distinct UmiRNAs and 80-99% of UmiRNAs in both the healthy controls and the renal disease patients. The majority of UmiRNAs displayed higher signal intensity in renal disease patients than in healthy controls, including 39 miRNAs exhibiting signal intensities 100 times greater than that in healthy controls. A different pattern of UmiRNAs was observed in each type of renal disease. The miRNA signal intensity of renal tissue and urine samples was significantly correlated. This study demonstrated that UmiRNAs presented different patterns corresponding to the type of renal disease. These results suggest that UmiRNAs can potentially be used as novel biomarkers for renal diseases.

Research Products

• [Journal Article] A microarray analysis of urinary microRNAs in renal diseases. (2014)
  • Author(s): Konta T, Ichikawa K, Suzuki K, Kudo K, Satoh H, Kamei K, Nishidate E, Kubota I.
  • Journal Title: Clin Exp Nephrol. Volume: - Issue: 5 Pages: 711-717
  • DOI: 10.1007/s10157-013-0906-5
  • Peer Reviewed
• [Journal Article] A microarray analysis of urinary microRNAs in renal diseases (2013)
  • Author(s): Konta T, Ichikawa K, Suzuki K, Kudo K, Satoh H, Kamei K, Nishidate E, Kubota
  • Journal Title: Clin Exp Nephrol Volume: Nov 6 (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Blood pressure in Japanese population with CKD (2012)
  • Author(s): Konta T, et al.
  • Journal Title: Am J Hypertens. Volume: 25 Issue: 3 Pages: 342-347
  • DOI: 10.1038/ajh.2011.217
  • Peer Reviewed
• [Journal Article] Significance of past history of renal failure for the detection of high-risk individuals for cardiovascular and end-stage renal disease : analysis of data from a nationwide health checkup (2011)
  • Author(s): Ichikawa K, Konta T, Ikeda A, Fujimoto S, Iseki K, Moriyama T, Yamagata K, Tsuruya K, Yoshida H, Asahi K, Kurahashi I, Ohashi Y, Watanabe T
  • Journal Title: Clin Exp Nephrol Volume: 15(6) Issue: 6 Pages: 841-7
  • DOI: 10.1007/s10157-011-0509-y
  • NAID: 10030341666
  • Peer Reviewed
• [Journal Article] Increases in urinary albumin and beta2-microglobulin are independently associated with blood pressure in the Japanese general population : the Takahata Study (2011)
  • Author(s): Mashima Y, Konta T, Kudo K, Takasaki S, Ichikawa K, Suzuki K, Shibata Y, Watanabe T, Kato T, Kawata S, Kubota I
  • Journal Title: Hypertens Res Volume: 34(7) Issue: 7 Pages: 831-5
  • DOI: 10.1038/hr.2011.42
  • NAID: 10031163265
  • Peer Reviewed
• [Journal Article] Polymorphism of proinflammatory cytokine genes and albuminuria in the Japanese general population: the Takahata study (2011)
  • Author(s): Mashima Y, Konta T, Kudo K, Suzuki K, Ikeda A, Ichikawa K, Shibata Y, Watanabe T, Tamiya G, Kato T, Kawata S, Kubota I.
  • Journal Title: Nephrol Dial Transplant Volume: 26(12) Issue: 12 Pages: 3902-3907
  • DOI: 10.1093/ndt/gfr105
  • Peer Reviewed
• [Presentation] 腎疾患患者における尿中microRNA発現プロファイル解析 (2013)
  • Author(s): 今田恒夫、佐藤紘子、工藤光介、鈴木和子、池田亜美、市川一誠、久保田功
  • Organizer: 第56回日本腎臓学会学術集会
  • Place of Presentation: 東京(東京国際ファーラム)
• [Presentation] THE PROFILE OF URINARY microRNAs IN RENAL DISEASES (2013)
  • Author(s): Konta T, Ichikawa K, Suzuki K, Kudo K, Satoh H, Kamei K, Nishidate E, Kubota I.
  • Organizer: World Congress Nephrology
  • Place of Presentation: 香港コンベンションセンター（中華人民共和国 香港特別行政区）
• [Presentation] 腎疾患患者における尿中microRNA発現プロファイル解析 (2013)
  • Author(s): 今田恒夫、他
  • Organizer: 第５６回日本腎臓学会学術総会
  • Place of Presentation: 東京ﾌｫｰﾗﾑ（東京都千代田区）
• [Presentation] 腎疾患患者における 尿中microRNA発現プロファイル解析 (2013)
  • Author(s): 今田恒夫
  • Organizer: 日本腎臓学会学術集会
  • Place of Presentation: 東京国際ﾌｫｰﾗﾑ (東京)
• [Presentation] THE PROFILE OF URINARY microRNAs IN RENAL DISEASES (2013)
  • Author(s): Tsuneo Konta
  • Organizer: World Congress of Nephrology
  • Place of Presentation: 香港ｺﾝﾍﾞﾝｼｮﾝｾﾝﾀｰ(中国）
• [Presentation] The urinary excretion of microRNAs in patients with renal diseases (2011)
  • Author(s): Tsuneo Konta, Kazuko Suzuki, Ami Ikeda, Yusuke Mashima, Kazunobu Ichikawa, Isao Kubota
  • Organizer: Annual meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia USA, (Pennsylvania Convention Center)
• [Presentation] Relation between renal parameters and u-miRNAs (2011)
  • Author(s): Tsuneo Konta, et al.
  • Organizer: American Society of Nephrology
  • Place of Presentation: Philadelphia, USA
• [Remarks]
  • URL: http://www.ichinai.com/