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High-resolution three-dimensional digital imaging of human renal microcirculation

Research Project

Project/Area Number 23591179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionUniversity of Tsukuba

Principal Investigator

UESUGI Noriko  筑波大学, 医学医療系, 准教授 (70279264)

Co-Investigator(Kenkyū-buntansha) NAGATA Michio  筑波大学, 医学医療系, 准教授 (10192238)
MONKA Sburo  日本歯科大学, 歯学部, 教授 (30028807)
Co-Investigator(Renkei-kenkyūsha) 島津 徳人  日本歯科大学, 歯学部, 講師 (10297947)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywordsヒト腎微小循環 / 3次元構築 / 腎癌非癌部 / 腎臓 / 微小循環 / ヒト腎臓 / 腎微小循環 / ヒト腎がん非癌部 / バーチャルスライド / ヒト腎内血管 / 腎内血管構造 / 慢性腎臓病
Outline of Final Research Achievements

We developed a new method to visualize human renal microvasculature in two- and three-dimensions (2D; 3D) using virtual microscopy. Serially cut 120-150 sections of paraffin-embedded human renal tissues from nephrectomized samples were double-immunostained with antibodies against CD34 (endothelial marker) and smooth muscle actin (arterial medial marker). Virtual microscopic images were processed with digital imaging analysis software. The resulting voxels (3 × 3 × 0.3 mm) included microvasculature from interlobular arteries to glomeruli, allowing visualization of 3D images of arteries and their direct connection to glomeruli and clarifying the underlying morphology of wall structures by corresponding 2D images.
The advantage of this method including visualizing the direct relationship between arterial changes and glomerular pathology by combining corresponding 2D and 3D images, which may provide new insight into the pathogenesis of arteriosclerotic nephrosclerosis.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2014 2013 2012

All Presentation (11 results) (of which Invited: 2 results)

  • [Presentation] Glomerular Microvascular Remodeling Cause Polar Vasculosis in Human Diabetic Nephropathy::With three-dimensional visualization2014

    • Author(s)
      Noriko Uesugi, Yoshihito Shimazu, Michio Nagata
    • Organizer
      アメリカ腎臓学会
    • Place of Presentation
      フィラデルフィア、アメリカ
    • Year and Date
      2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 腎疾患の病態解明における分子形態学の現状と今 後の展望2014

    • Author(s)
      上杉 憲子
    • Organizer
      第46回日本臨床分子形態学会ワ-クショップ
    • Place of Presentation
      東京
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] バーチャルスライドシステムを用いたヒト腎組 織標本を用いた糸球体,血管,尿細管システム の 3 次元構築2014

    • Author(s)
      上杉憲子 島津徳人  長田道夫
    • Organizer
      第53回日本腎臓学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-07-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒト腎組織の血管・糸球体・尿細管システムの 3 次元構築2014

    • Author(s)
      上杉憲子 島津徳人  長田道夫
    • Organizer
      第103回 日本病理学会 総会
    • Place of Presentation
      広島
    • Year and Date
      2014-04-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] CKDでは血管障害がAtubular glomeruliを惹起する2013

    • Author(s)
      上杉憲子、島津徳人、青葉孝昭、長田道夫
    • Organizer
      第56回日本腎臓学会学術総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 慢性腎臓病(CDK)における糸球体尿細管接合部異常と動脈硬化の関与2013

    • Author(s)
      上杉憲子、島津徳人、青葉孝昭、長田道夫
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] Vascular Injury Promotes Glomerulotubular Junction Abnormalities (GTJA) which May Progress Chronic Kidney Disease (CKD): Three-Dimensional (3D) Inspection of Vasculo-Glomerular Alterations2013

    • Author(s)
      N.Uesugi, Y.Shimazu, T Aoba,  M.Nagata
    • Organizer
      American Society of Nephrology kidney week 2013
    • Place of Presentation
      アメリカ アトランタ
    • Related Report
      2013 Research-status Report
  • [Presentation] 腎内動脈硬化の病理と問題点2013

    • Author(s)
      上杉憲子
    • Organizer
      動脈硬化学会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 腎内血管走行の3次元構築の試み:弓状小葉間動脈の分枝と微小血管網2012

    • Author(s)
      上杉憲子、島津徳人、田谷 雄二、青葉孝昭、長田道夫
    • Organizer
      日本腎臓学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 腎内血管走行の 3 次元構築 : 弓状小葉間動脈の分枝と微小血管網2012

    • Author(s)
      上杉憲子、島津徳人、青葉孝昭、長田道夫
    • Organizer
      日本病理学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Three-Dimensional Visualization of Vasculo-Glomerular Alterations with Progression of Chronic Kidney Disease2012

    • Author(s)
      N.Uesugi, T.Shimazu,T. Aoba, M.Nagata
    • Organizer
      American Society of Nephrology (ASN) Kidney Week 2012 Annual Meeting
    • Place of Presentation
      San Diego, Californ ia、USA
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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