Tyrosine phosporylation and de-phosphorylation in glomerular barrier function
| Project/Area Number |
23591180
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Gunma University |
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Principal Investigator |
NOJIMA YOSHIHISA 群馬大学, 医学(系)研究科(研究院), 教授 (90201699)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 糸球体上皮細胞 / チロシン脱リン酸化 / SIRP-α / 蛋白尿 / 糸球体バリア機能 / チロシン脱リン酸化酵素 / たんぱく尿 / CD47 |
|---|
| Research Abstract |
SIRPa is a transmembrane protein with tyrosine phosphorylation sites in its cytoplasmic region where two tyrosine phosphatases, SHP-1/SHP-2, bind to in a phosphorylation-dependent manner, and is expressed by podocytes. We examined the role of SIRPa in podocytes using knock-in mice expressing mutant SIRPa that lacks a cytoplasmic region. While there was no morphological abnormalities in the kidneys of the SIRPa-mutant mice on light microscopy, electron microscopic examination revealed abnormal podocytes with irregular major processes and wider and flattened foot processes in the SIRPa-mutant mice. Impaired renal functions and slight albuminuria were demonstrated in the mutant mice. In addition, adriamycin injection induced massive albuminuria along with focal glomerulosclerosis in the SIRPa-mutant mice, while their wild-type counterparts were resistant to adriamycin. These data indicate that SIRPa is involved in the regulation of podocyte structure and function as a filtration barrier.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] SIRPαsignaling regulates podocyte structure and function2013
Author(s)
Takahashi S, Tomioka M, Hiromura K, Sakairi T, Hamatani H, Watanabe M, Ikeuchi H, Kaneko Y, Maeshima A, Aoki T, Ohnishi H, Matozaki T, Nojima Y
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Journal Title
Am J Physiol Renal Physiol
Volume: 305
Related Report
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[Journal Article] SIRPα signaling regulates podocyte structure and function.2013
Author(s)
Takahashi S, Tomioka M, Hiromura K, Sakairi T, Hamatani H, Watanabe M, Ikeuchi H, Kaneko Y, Maeshima A, Aoki T, Ohnishi H, Matozaki T, Nojima Y
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Journal Title
Am J Physiol Renal Physiol.
Volume: 305
Related Report
Peer Reviewed
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