• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The studies on the roles of transcriptional factors in pathogenesis of vascular calcification by chronic kidney disease and their application for the therapy

Research Project

Project/Area Number 23591200
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionKeio University

Principal Investigator

HAYASHI Matsuhiko  慶應義塾大学, 医学部, 教授 (60129608)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Tadashi  慶應義塾大学, 医学部, 講師 (00306713)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords血管石灰化 / 慢性腎臓病 / NFkappaB / Klf4 / 転写調節因子 / NFkB / リン代謝
Research Abstract

To reveal the mechanisms which induce vascular calcification in the patients with chronic kidney disease, roles of transcriptional factors which are related to vascular calcification are studied in the present study. We found that Klf4, a transcriptional factor, plays important roles in transformation of vascular smooth muscle cells to osteocyte-like cells by hyperphosphathemia in the in vivo and in vitro experiments. It was also shown that by the same experimental system hyperglycemia is not a significant factor for worsening of vascular calcification. In addition, by vascular smooth muscle cell specific inactivation of NF-kB activity in mouse, endothelial thickening arteriosclerosis by vascular injury was ameliorated. Currently, we are examining the roles of NF-kB in the pathogenesis of vascular calcification by chronic renal failure, using these genetically modified mouse.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2014 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (11 results)

  • [Journal Article] Deletion of Kruppel-like factor 4 in endothelial and hematopoietic cells enhances neointimal formation following vascular injury.2014

    • Author(s)
      Yoshida T, Yamashita M, Horimai C, Hayashi M.
    • Journal Title

      J Am Heart Assoc.

      Volume: 3 Issue: 1

    • DOI

      10.1161/jaha.113.000622

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of Kruppel-Like Factor 4 and its Binding Proteins in Vascular Disease.2014

    • Author(s)
      Yoshida T, Hayashi M.
    • Journal Title

      J Atheroscler Thromb

      Volume: 未定

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High glucose concentration does not modulate the formation of arterial medial calcification in experimental uremic rats.2013

    • Author(s)
      Yoshida T, Yamashita M, Horimai C, Hayashi M.
    • Journal Title

      J Vasc Res

      Volume: 50 Issue: 6 Pages: 512-520

    • DOI

      10.1159/000355263

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Smooth muscle-selective inhibition of nuclear factor-κB attenuates smooth muscle phenotypic switching and neointima formation following vascular injury.2013

    • Author(s)
      Yoshida T, Yamashita M, Horimai C, Hayashi M.
    • Journal Title

      J Am Heart Assoc

      Volume: 2 Issue: 3

    • DOI

      10.1161/jaha.113.000230

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Krüppel-like factor 4 contributes to high phosphate-induced phenotypic switching of vascular smooth muscle cells into osteogenic cells.2012

    • Author(s)
      Tadashi Yoshida, Maho Yamashita, Matsuhiko Hayashi
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Issue: 31 Pages: 25706-25714

    • DOI

      10.1074/jbc.m112.361360

    • Related Report
      2012 Research-status Report
  • [Journal Article] Role of Krüppel-Like Factor 4 and its Binding Proteins in Vascular Disease

    • Author(s)
      Yoshida T, Hayashi M
    • Journal Title

      J Atheroscler Thromb

      Volume: (in press)

    • NAID

      130004444739

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Krüppel-like cactor 4 in endothelial cells protects mice from renal ischemia-reperfusion injury2013

    • Author(s)
      Tadashi Yoshida, Maho Yamashita, Matsuhiko Hayashi
    • Organizer
      the 46th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Atlanta, USA
    • Year and Date
      2013-11-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 腎不全ラットの血管石灰化形成におけるグルコース濃度の影響2013

    • Author(s)
      吉田理、山下真帆、林松彦
    • Organizer
      第58回日本透析医学会学術集会・総会
    • Place of Presentation
      福岡市
    • Year and Date
      2013-06-23
    • Related Report
      2013 Final Research Report
  • [Presentation] Kruppel-like cactor 4 in endothelial cells protects mice from renal ischemia-reperfusion injury2013

    • Author(s)
      Tadashi Yoshida, Maho Yamashita, Matsuhiko Hayashi
    • Organizer
      the 46th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Atlanta, Georgia, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] High Glucose May Not Directly Affect High Phosphate-Induced Calcification of Vascular Smooth Muscle Cells2012

    • Author(s)
      Tadashi Yoshida, Maho Yamashita, Matsuhiko Hayashi
    • Organizer
      the 45h Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2012-11-03
    • Related Report
      2013 Final Research Report
  • [Presentation] リン負荷による血管石灰化におけるグルコース濃度の影響2012

    • Author(s)
      吉田理、山下真帆、堀米ちひろ、林松彦
    • Organizer
      第57回日本透析医学会学術集会・総会
    • Place of Presentation
      札幌市
    • Year and Date
      2012-06-22
    • Related Report
      2013 Final Research Report
  • [Presentation] リポポリサッカライドは血管平滑筋細胞石灰化を促進する2012

    • Author(s)
      林松彦、高松一郎、堀米ちひろ、吉田理
    • Organizer
      第55回日本腎臓学会学術総会
    • Place of Presentation
      横浜市
    • Year and Date
      2012-06-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Kruppel-Like Factor 4 Mediates High Phosphate-Induced Conversion of Vascular Smooth Muscle Cells into Osteoblast-Like Cells2011

    • Author(s)
      Tadashi Yoshida, Maho Yamashita, Matsuhiko Hayash
    • Organizer
      the 44th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2011-11-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Lipopolysaccharide and TNF-αAggravate Calcification in Vascular Smooth Muscle Cells by High Inorganic Phosphate and High Calcium Concentration Media2011

    • Author(s)
      Matsuhiko Hayashi, Ichiro Takamatsu, Chihiro Horimai, Tadashi Yoshida, Toshifumi Wakamatsu
    • Organizer
      the 44th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2011-11-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Kruppel-Like Factor 4 Mediates High Phosphate-Induced Conversion of Vascular Smooth Muscle Cells into Osteoblast-Like Cells2011

    • Author(s)
      Tadashi Yoshida, Maho Yamashita, Matsuhiko Hayashi
    • Organizer
      2011年アメリカ腎臓学会学年次総会
    • Place of Presentation
      アメリカ合衆国フィラデルフィア
    • Related Report
      2011 Research-status Report
  • [Presentation] 腎不全ラットの血管石灰化形成におけるグルコース濃度の影響

    • Author(s)
      吉田 理、山下 真帆、林 松彦
    • Organizer
      第58回日本透析医学会学術集会・総会
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] High glucose may not directly affect high phosphate-induced calcification of vascular smooth muscle cells.

    • Author(s)
      Tadashi Yoshida, Maho Yamashita, Matsuhiko Hayashi
    • Organizer
      45th Annual Meeting of the American Society of Nephrology
    • Place of Presentation
      San Diego
    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi