Analysis of paracellular sodium ion transport in the thin ascending limb of Henle's loop

• Project/Area Number: 23591211
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Nihon University
• Principal Investigator: MORIMOTO TETSUJI 日本大学, 医学部, 助教 (10344657)
• Co-Investigator(Kenkyū-buntansha): KONDO Yoshiaki 日本大学, 医学部, 教授 (00221250) ; UCHIDA Shinichi 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (50262184)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 細いヘンレの上行脚 / 細胞間隙 / ナトリウムイオン輸送 / ナトリウム輸送 / ATL / インスリン / 尿細管 / クローディン4 / アルドステロン / 拡散電位 / 国際情報交流

Research Abstract

We analyzed paracellular sodium ion transport mechanism in the thin ascending limb, which plays an important role in mammalian urine-concentrating mechanism, using in vitro microperfusion technique. We examined whether aldosterone and insulin affected the transepithelial voltages or not. However, the transepithelial voltages remained unchanged. These results meant that both of them did not affect a sodium ion transport in this segment. Besides, according to just a few experiments, insulin like growth factor - 1 also suggested the same result.