Possible roles of tumor necrosis factor-alpha and angiotensin II type 1 receptor on high glucose-induced damage in renal proximal tubular cells
| Project/Area Number |
23591219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kawasaki Medical School (2013-2014)
Kochi University (2011-2012) |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 近位尿細管 / グルコース / アンジオテンシン受容体 / 酸化ストレス / 腫瘍壊死因子 / 腎尿細管細胞 / 腎尿細管障害 / 細胞障害 |
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| Outline of Final Research Achievements |
High glucose treatment (30 mM) significantly increased N-acetyl-beta-glucosaminidase (NAG) release, tumor necrosis factor (TNF)alpha/angiotensin II concentrations in cell media and p22phox protein levels compared with those in regular glucose medium (5.6 mM). Candesartan, an angiotensin II type 1 receptor (AT1R) blocker, showed a significant reduction on high glucose-induced NAG release, TNFalpha concentrations and p22phox protein levels in human renal proximal tubular epithelial cells (HK2 cells). In addition, significant decreases of NAG release, TNFalpha concentrations and p22phox protein levels in HK2 cells were observed in high glucose-treated group with thalidomide. AT1R knockdown with siRNA markedly reversed high glucose, angiotensin II or TNFalpha-induced p22phox protein levels in HK2 cells. TNFalpha may be involved in high glucose-induced renal tubular damage in HK2 cells possibly via AT1 receptor signaling.
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Report
(5 results)
Research Products
(6 results)
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[Presentation] EFFECT OF ANGIOTENSIN II BLOCKERS ON HIGH GLUCOSE-INDUCED CELL DAMAGE THROUGH TUMOR NECROSIS FACTOR ALPHA IN RENAL PROXIMAL TUBULAR CELLS2012
Author(s)
TOSHIHIRO TAKAO, TARO HORINO, YOSHIKO SHIMAMURA, KOJI OGATA, KOUSUKE INOUE, YOSHINORI TANIGUCHI, TAKAFUMI TAGUCHI, TORU KAGAWA, YOSHIO TERADA
Organizer
The 22nd Scientific Meeting of the European Society of Hypertension
Place of Presentation
ICC London, ロンドン, UK
Related Report
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