| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
Recent studies suggest the roles of osteocyte in phosphate metabolism, although the underlying mechanisms remain unclear. In the current study, the roles of osteocytes in phosphate metabolism were investigated using the osteoblasts and osteocytes isolated from mouse long bones. The expression of Fgf23, Dmp1, Phex and Fam20c was higher in osteocytes than osteoblasts. In the osteocytes isolated from Phex-deficient Hyp mice, the expressions of Dmp1, Fam20c and Pit1 as well as that of Fgf23 were up-regulated. 24-hour treatment with 1,25-dihydroxyvitamin D markedly increased the expression of Fgf23. On the other hand, 24-hour treatment with high phosphate did not alter the expression of Fgf23, although it markedly increased the expression of Dmp1. These results suggest that chronic phosphate load is required for the increase of FGF23 production.
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