Molecular Mechanism for Phosphate Sensing and Bone-Kidney Functional Interaction
| Project/Area Number |
23591227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Research Institute, Osaka Medical Center for Maternal and Child Health |
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Principal Investigator |
MICHIGAMI Toshimi 地方独立行政法人大阪府立病院機構大阪府立母子保健総合医療センター(研究所), 環境影響部門, 部長 (00301804)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | リン代謝 / 骨細胞 / FGF23 / DMP1 / PHEX / ビタミンD / FAM20C |
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| Research Abstract |
Recent studies suggest the roles of osteocyte in phosphate metabolism, although the underlying mechanisms remain unclear. In the current study, the roles of osteocytes in phosphate metabolism were investigated using the osteoblasts and osteocytes isolated from mouse long bones. The expression of Fgf23, Dmp1, Phex and Fam20c was higher in osteocytes than osteoblasts. In the osteocytes isolated from Phex-deficient Hyp mice, the expressions of Dmp1, Fam20c and Pit1 as well as that of Fgf23 were up-regulated. 24-hour treatment with 1,25-dihydroxyvitamin D markedly increased the expression of Fgf23. On the other hand, 24-hour treatment with high phosphate did not alter the expression of Fgf23, although it markedly increased the expression of Dmp1. These results suggest that chronic phosphate load is required for the increase of FGF23 production.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Dysregulated gene expression in the primary osteoblasts and osteocytes isolated from hypophosphatemic Hyp mice.2014
Author(s)
Miyagawa K, Yamazaki M, Kawai M, Nishino J, Koshimizu T, Ohata Y, Tachikawa K, Mikuni-Takagaki Y, Kogo M, Ozono K, Michigami T
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Journal Title
PLoS One
Volume: 9
Issue: 4
Pages: 0-0
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] An overgrowth disorder associated with excessive production of cGMP due to a gain-of-function mutation of the natriuretic peptide receptor 2 gene.2012
Author(s)
Miura K, Namba N, Fujiwara M, Ohata Y, Ishida H, Kitaoka T, Kubota T, Hirai H, Higuchi C, Tsumaki N, Yoshikawa H, Sakai N, Michigami T, Ozono K.
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Journal Title
PLoS One
Volume: 7
Issue: 8
Pages: e42180-e42180
DOI
Related Report
Peer Reviewed
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[Presentation] Fetal stage-specific mineral metabolism in Hyp mice is associated with effects of FGF23 on placenta2013
Author(s)
Ohata Y, Yamazaki M, Kawai M, Tachikawa K, Koinuma T, Miyagawa K, Kimoto A, Nakayama M, Namba N, Yamamoto H, Ozono K, Michigami T
Organizer
35th Annual Meeting of the American Society for Bone and Mineral Research
Place of Presentation
Bartimore
Related Report
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[Presentation] Fetal stage-specific mineral metabolism in Hyp mice is associated with effects of FGF23 on placenta.2013
Author(s)
Ohata Y, Yamazaki M, Kawai M, Tachikawa K, Koinuma T, Miyagawa K, Kimoto A, Nakayama M, Namba N, Yamamoto H, Ozono K, Michigami T
Organizer
35th Annual Meeting of the American Society for Bone and Mineral Research
Place of Presentation
Bartimore, USA
Related Report
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