Analysis of molecular functions of presenilin in CSF phosphorylation of tau

• Project/Area Number: 23591231
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Gunma University
• Principal Investigator: IKEDA MASAKI 群馬大学, 医学部附属病院, 講師 (50222899)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Koichi 群馬大学, 大学院医学系研究科, 教授 (00124652) ; YAMAZAKI Tsuneo 群馬大学, 大学院保健学研究科, 教授 (80200658) ; FUJITA Yukio 群馬大学, 大学院医学系研究科, 講師 (70420172) ; MAKIOKA Kouki 群馬大学, 医学部附属病院, 助教 (10420176) ; FURUTA Natsumi 群馬大学, 医学部附属病院, 助教 (80625583) ; 平柳 公利 群馬大学, 医学部附属病院, 助教 (20701395)
• Co-Investigator(Renkei-kenkyūsha): SHOJI Mikio 弘前大学, 大学院医学研究科 (60171021)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: プレセニリン / タウ / 脳脊髄液 / アルツハイマー病 / 遺伝子変異 / アミロイドβ / 髄液 / リン酸化タウ / 神経細胞死

Research Abstract

We confirmed 6 AD different PS1 mutations (T99A, H131R, H163R, M233L, L219R, V272A) in 6 independent Japansese FAD families. They showed slight elevation of CSF ptau (199, 181), reduction of Ab1-42, Ab1-40 and Ab1-38. While CSF Ab1-42 remained same level, CSF Ab1-40 and Ab1-38 gradually decreased. This may suggest active sites of gamma-secretase are different between sporadic AD and PS1 mutatnt AD patients, and Ab processing might be changing in aging of PS1 mutant AD.

Research Products

• [Journal Article] Lack of genetic association between TREM2 and late-onset Alzheimer’s disease in a Japanese population (2014)
  • Author(s): Akinori Miyashitaa, Yanan Wen 他
  • Journal Title: J Alzheimer's Dis. Volume: 印刷中 Issue: 4 Pages: 1301-1308
  • DOI: 10.3233/jad-140225
  • Peer Reviewed / Open Access
• [Journal Article] Immunolicalization of Smurf1 in Hirano bodies (2014)
  • Author(s): Makioka K, Yamazaki T, Takatama M, Ikeda M, Okamoto K
  • Journal Title: J Neurol Sci Volume: 336 Pages: 24-8
  • Peer Reviewed
• [Journal Article] Immunolocalization of Smurf1 in Hirano bodies. (2014)
  • Author(s): Makioka K, Yamazaki T, Takatama M, Ikeda M, Okamoto K
  • Journal Title: J Neurol Sci Volume: 336 Issue: 1-2 Pages: 24-28
  • DOI: 10.1016/j.jns.2013.09.028
  • Peer Reviewed / Open Access
• [Journal Article] Lack of genetic association between TREM2 and late-onset Alzheimer’s disease in a Japanese population (2014)
  • Author(s): Miyashita A, Wen Y, Kitamura N, Harigaya Y, Ikeda M, Amari M, Yamaguchi H, Kanazawa I, Ihara Y, Ikeuchi T, Kuwano R
  • Journal Title: Journal of Alzheimer's Disease Volume: April 24 Pages: 1-12
  • Peer Reviewed
• [Journal Article] CSF phosphorylated tau and Aβ(1-38/1-40/1-42) in Alzheimer's disease with novel PS1 mutations (2013)
  • Author(s): Ikeda M, Fujita Y, Makioka K, Yamazaki T, Yamaguchi H
  • Journal Title: Amyloid Volume: 20 Pages: 107-12
  • Peer Reviewed
• [Journal Article] Neck weakness is a potent prognostic factor in sporadic amyotrophic lateral sclerosis patients. (2013)
  • Author(s): Nakamura R, Atsuta N, Watanabe H, Hirakawa A, Watanabe H, Ito M, Senda J, Katsuno M, Tanaka F, Izumi Y, Morita M, Ogaki K, Taniguchi A, Aiba I, Mizoguchi K, Okamoto K, Hasegawa K, Aoki M, Kawata A, Abe K, Oda M, Konagaya M, Imai T, Nakagawa M, Tsuji S, Kaji R, Nakano I, Sobue G.
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: 84 Issue: 12 Pages: 1365-1371
  • DOI: 10.1136/jnnp-2013-306020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reduced expression of BTBD10 in anterior horn cells with Golgi fragmentation and pTDP-43-positive inclusions in patients with sporadic amyotrophic lateral sclerosis (2013)
  • Author(s): Furuta N, Makioka K, Fujita Y, Ikeda M, Takatama M, Matsuoka M, Okamoto K
  • Journal Title: Neuropathology Volume: 33 Issue: 4 Pages: 397-404
  • DOI: 10.1111/neup.12010
  • Peer Reviewed
• [Journal Article] SORL1 Is Genetically Associated with Late-Onset Alzheimer’s Disease in Japanese, Koreans and Caucasians (2013)
  • Author(s): Miyashita A, Koike A, Jun G, Kawarabayashi T, Shoji M,Arai H, Asada T, Harigaya Y, Ikeda M, Amari M, Ikeuchi T, St. George-Hyslop P,
  • Journal Title: PLoS One Volume: 8 Issue: 4 Pages: e58618-e58618
  • DOI: 10.1371/journal.pone.0058618
  • Peer Reviewed
• [Journal Article] Changes in the Clinical Features of Amyotrophic Lateral Sclerosis in Rural Japan (2013)
  • Author(s): Furuta N, Ikeda M, Fujita Y, Okamoto K(2
  • Journal Title: Internal Medicine Volume: 52 Issue: 15 Pages: 1691-1696
  • DOI: 10.2169/internalmedicine.52.0195
  • NAID: 130003365732
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Cerebrospinal fluid levels of phosphorylated tau and Aβ1-38/Aβ1-40/Aβ1-42 in Alzheimer's disease with PS1 mutations. (2013)
  • Author(s): Ikeda M, Yonemura K, Kakuda S, Tashiro Y, Fujita Y, Takai E, Hashimoto Y, Makioka K, Furuta N, Ishiguro K, Maruki R, Yoshida J, Miyaguchi O, Tsukie T, Kuwano R, Yamazaki T, Yamaguchi H, Amari M, Takatama M, Harigaya Y, Okamoto K
  • Journal Title: Amyloid Volume: 20 Issue: 2 Pages: 107-712
  • DOI: 10.3109/13506129.2013.790810
  • Peer Reviewed
• [Journal Article] A novel GJA1 mutation in oculodentodigital dysplasia with progressive spastic paraplegia and sensory deficits (2013)
  • Author(s): Furuta N, Ikeda M, Hirayanagi K, Fujita Y, Amanuma M, Okamoto K
  • Journal Title: Internal Medicine Volume: 51(1) Pages: 93-8
  • NAID: 130002062415
  • Peer Reviewed
• [Journal Article] Cerebral encephalopathy with amyloid angiopathy and numerous plaques in low levels of CSF Aβ1-40/Aβ1-42 (2012)
  • Author(s): Ikeda M, Makioka K, Okamoto K
  • Journal Title: Amyloid Volume: 19 Pages: 186-90
  • Peer Reviewed
• [Journal Article] Activation and alteration of lysosomes in multiple system atrophy (2012)
  • Author(s): Makioka K, Yamazaki T, Takatama M, Nakazato Y, Okamoto K
  • Journal Title: Neuroreport Volume: Vol.23 Issue: 5 Pages: 270-276
  • DOI: 10.1097/wnr.0b013e3283503e4f
  • Peer Reviewed
• [Journal Article] 原発性マクログロブリン血症に続発し,レナリドマイドで治療したPOEMS症候群の46歳男性 (2012)
  • Author(s): 古田夏海,池田将樹,藤田行雄,岡本幸市
  • Journal Title: 臨床神経学 Volume: 52 Pages: 186-9
  • Peer Reviewed
• [Journal Article] SIADHで発症し,頸髄に短い非連続性の偏在性病変を認めたNMO spectrum disordersの高齢男性症例 (2012)
  • Author(s): 古田夏海,池田将樹,岡本幸市
  • Journal Title: Kitakanto Med J Volume: 62 Pages: 311-4
  • NAID: 130002096486
  • Peer Reviewed
• [Journal Article] Encephalopathy with amyloid angiopathy and numerous amyloid plaques with low levels of CSF Aβ1-40/Aβ1-42 (2012)
  • Author(s): Masaki Ikeda, Kimitoshi Hirayanagi, Motonobu Arai, Satoko Kakuda, Kouki Makioka, Yukio Fujita, Masakuni Amari, Masakitsu Takatama, Yoichi Nakazato, Koichi Okamoto
  • Journal Title: Amyloid Volume: Early online1-5 Issue: 4 Pages: 1350-1356
  • DOI: 10.3109/13506129.2012.725113
  • Peer Reviewed
• [Journal Article] A novel GJA1 mutation in oculodentodigital dysplasia with progressive spastic paraplegia and sensory deficits (2012)
  • Author(s): Furuta N, Ikeda M, Fujita Y, Hirayanagi K, Amanuma M, Okamoto K
  • Journal Title: Internal Medicine Volume: 51 Pages: 93-98
  • NAID: 130002062415
  • Peer Reviewed
• [Journal Article] fox-pigeon imitation test (YFPIT) for dementia in clinical practice (2011)
  • Author(s): Yamaguchi H, Takahashi S, Kosaka K, Okamoto K, Yamazaki T, Ikeda M, Osawa M, Amari M, Harigaya Y, Awata S, Maki Y, Yamaguchi
  • Journal Title: Psychogeriatrics Volume: Vol.11 Issue: 4 Pages: 221-226
  • DOI: 10.1111/j.1479-8301.2011.00373.x
  • Peer Reviewed
• [Journal Article] Different clinical and neuropathologic phenotypes of familial ALS with A315E TARDBP mutation (2011)
  • Author(s): Fujita Y, Ikeda M, Okamoto K
  • Journal Title: Neurology Volume: 77 Issue: 15 Pages: 1427-31
  • DOI: 10.1212/wnl.0b013e318232ab87
  • Peer Reviewed
• [Journal Article] Yamaguchi fox-pigeon imitation test (YFPIT) for dementia in clinical practice (2011)
  • Author(s): Yamaguchi H, Takahashi H, Kosaka K, Okamoto K, Yamazaki T, Ikeda M, Osawa M, Amari M, Harigaya Y, Awata S, Maki Y
  • Journal Title: Psychogeriatrics Volume: 11 Pages: 221-226
  • Peer Reviewed
• [Journal Article] Different clinical and neuropathologic phenotypes of familial ALS with A315E TARDBP mutation (2011)
  • Author(s): Fujita Y, Ikeda M, Yanagisawa T, Senoo Y, Okamoto K
  • Journal Title: Neurology Volume: 77 Pages: 1427-1427
• [Presentation] 脳アミロイドアンギオパチー関連脳内出血を認めたアルツハイマー病症例におけるC PiB-PETの検討 (2014)
  • Author(s): 池田将樹、田代裕一、津田和寿、有坂由紀子、甘利雅邦、高玉真光、岡本幸市、樋口徹也、対馬義人、池田佳生
  • Organizer: 第39回日本脳卒中学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2014-03-15
• [Presentation] PES症候群を呈したFTDP-17の1例 (2014)
  • Author(s): 浦茂久、倉内麗徳、河端聡、黒島研美、吉田一人、大槻美佳、高井恵理子、池田佳生、池田将樹
  • Organizer: 日本神経学会北海道地方会
  • Place of Presentation: 札幌
  • Year and Date: 2014-03-08
• [Presentation] 脳アミロイドアンギオパチー関連脳内出血を認めたアルツハイマー病症例における11C-PiB PETの検討 (2014)
  • Author(s): 池田将樹、田代裕一1、津田和寿、小平明果、有坂由紀子、甘利雅邦、高玉真光、岡本幸市、樋口徹也、対馬義人、池田佳生
  • Organizer: 第39回日本脳卒中学会総会（STROKE2014）
  • Place of Presentation: 大阪国際会議場
• [Presentation] 群馬県における脳卒中地域連携クリニカルパスの共通化と改良 (2013)
  • Author(s): 池田将樹、朝倉健、猿木和久、瀧口道生、栗原秀行、須賀一夫
  • Organizer: 第31回日本神経治療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2013-11-23
• [Presentation] PiB-PET所見などによりアルツハイマー病と考えられたposterior cortical atrophyの3症例 (2013)
  • Author(s): 池田将樹,田代裕一,荒木邦彦,黒瀬佐知子,高井恵理子,普神直子,津田和寿,樋口徹也,岡本幸市,池田佳生,対馬義人
  • Organizer: 第32回日本認知症学会学術集会
  • Place of Presentation: 松本
  • Year and Date: 2013-11-08
• [Presentation] アミロイドアンギオパチー関連脳内出血を認めたアルツハイマー病症例における^11C-PiB PETの検討 (2013)
  • Author(s): 池田将樹、田代裕一、津田和寿、小平明果、有坂由紀子、甘利雅邦、高玉真光、岡本幸市、樋口徹也、池田佳生、対馬義人
  • Organizer: 日本血管認知症研究会
  • Place of Presentation: 東京
  • Year and Date: 2013-08-10
• [Presentation] Analysis of cognition, CSF and neuroimaging in cases of progressive aphasia (2013)
  • Author(s): Ikeda M, Tashiro Y, Tsuda K, Takai E, Kurose S, Fugami N, Amari M, Harigaya Y, Yamazaki T, Yamaguchi H, Takatama M, Okamoto K, Higuchi T, Tsushima Y, Ikeda Y
  • Organizer: AAIC2013
  • Place of Presentation: 米国、ボストン
  • Year and Date: 2013-07-17
• [Presentation] 進行性失語症を呈する症例の認知機能、脳脊髄液および神経放射線学的検討 (2013)
  • Author(s): 池田将樹、田代裕一、津田和寿、小平明果、有坂由紀子、山口晴保、水野裕司、山崎恒夫、樋口徹也、対馬義人、岡本幸市
  • Organizer: 第54回日本神経学会総会
  • Place of Presentation: 東京
  • Year and Date: 2013-06-01
• [Presentation] 進行性失語症と考えられる症例の認知機能、脳脊髄液および神経放射線学的検討 (2013)
  • Author(s): 池田将樹、田代裕一、津田和寿、小平明果、有坂由紀子、山口晴保、水野裕司、山崎恒夫、樋口徹也、対馬義人、岡本幸市
  • Organizer: 第54回日本神経学会学術大会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] 群馬県における脳卒中地域連携クリニカルパスの作成と共通化に関する活動報告 (2013)
  • Author(s): 池田将樹, 朝倉 健, 猿木和久, 瀧口道生 , 栗原秀行, 須賀一夫
  • Organizer: 第31回日本神経治療学会
  • Place of Presentation: 東京ドームホテル
• [Presentation] PiB-PET所見などよりアルツハイマー病と考えられたposterior cortical atrophyの3 (2013)
  • Author(s): 池田将樹，田代裕一，荒木邦彦，黒瀬佐知子，高井恵理子1，普神直子，津田和寿，池田佳生，樋口徹也，岡本幸市，対馬義人
  • Organizer: 第32回日本認知症学会学術集会
  • Place of Presentation: キッセイ文化ホール
• [Presentation] 進行性失語症と考えられる3症例の認知機能、脳脊髄液および神経放射線学的検討 (2012)
  • Author(s): 池田将樹、田代裕一、津田和寿、小平明果、有坂由紀子、水野裕司、山崎恒夫、樋口徹也、対馬義人、岡本幸市
  • Organizer: 第31回日本認知症学会学術集会
  • Place of Presentation: つくば
  • Year and Date: 2012-10-26
• [Presentation] Cerebral encephalopathy with cerebral amyloid angiopathy and numerous amyloid plaques presenting aberrant low levels of CSF Aβ1-40/1-42 (2012)
  • Author(s): Ikeda M, Hirayanagi K, Arai M, Kakuda S, Makioka K, Furuta N, Kasahara H, Fujita Y, Amari M, Takatama M, Nakazato Y, Okamoto K
  • Organizer: AAIC2012
  • Place of Presentation: カナダ、バンクーバー
  • Year and Date: 2012-07-15
• [Presentation] ADとDLBにおける脳脊髄液のAβ(Aβ1-38,Aβ1-40,Aβ1-42)とリン酸化タウの検討 (2012)
  • Author(s): 池田将樹、米村公江、藤田行雄、田代裕一、橋本由紀子、角田聡子、高井恵理子、山崎恒夫、高玉真光、甘利雅邦、針谷康夫、岡本幸市
  • Organizer: 第53回日本神経学会総会
  • Place of Presentation: 東京
  • Year and Date: 2012-05-23
• [Presentation] Cerebral encephalopathy with cerebral amyloid angiopathy and numerous amyloid plaques presenting aberrant low levels of CSF Aβ-40/1-42 (2012)
  • Author(s): M. Ikeda, K. Hirayanagi, M Arai, S. Kakuda, K. Makioka, N. Furuta, H. Kasahara, Y. Fujita, M. Amari, M. Takatama, Y. Nakazato, K. Okamoto
  • Organizer: AICC2012
  • Place of Presentation: Vancouver, Canada
• [Presentation] ADとDLBにおける脳脊髄液Aβ（Aβ1-38，Aβ1-40，Aβ1-42）とリン酸化タウの検討 (2012)
  • Author(s): 池田将樹，米村公江，藤田行雄，田代裕一，橋本由紀子1，角田聡子，高井恵理子，山崎恒夫，高玉真光，甘利雅邦，針谷康夫，岡本幸市
  • Organizer: 第53回日本神経学会総会
  • Place of Presentation: 東京
• [Presentation] PS1変異AD7症例の脳脊髄液におけるリン酸化タウとAβアミロイド(Aβ1-38,Aβ1-40,Aβ1-42)の検討 (2011)
  • Author(s): 池田将樹、米村公江、角田聡子、藤田行雄、橋本由紀子、田代裕一、石黒幸一、山崎恒夫、高玉真光、甘利雅邦、針谷康夫、岡本幸市
  • Organizer: 第30回日本認知症学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-12
• [Presentation] Diffuse reduction of cerebral blood flow, cognitive impairment and depressive mood in sporadic SCD cases with APTX and SETX mutations (2011)
  • Author(s): Ikeda M, Amari M, Watanabe M, Shimazaki H, Takatama M, Takiyama Y, Harigaya Y, Okamoto K
  • Organizer: 4th International Congress of the Society for Research on the Cerebellum
  • Place of Presentation: 東京
  • Year and Date: 2011-09-18
• [Presentation] New evidence of temporal kinetics of CSF Aβ1-38/1-40/1-42 and phosphorylated tau in seven FAD cases with novel PSEN1 mutations (2011)
  • Author(s): Ikeda M, Yonemura K, Kakuda S, Fujita Y, Hashimoto Y, Tashiro Y, Ishiguro K, Amari M, Takatama M, Harigaya Y, Okamoto K
  • Organizer: AAIC2011
  • Place of Presentation: フランス、パリ
  • Year and Date: 2011-07-20
• [Presentation] (2011)
  • Author(s): 池田将樹、角田聡子、藤田行雄、橋本由紀子、米村公江、石黒幸一、山崎恒夫、甘利雅邦、針谷康夫、岡本幸市
  • Organizer: 第52回日本神経学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-05-20
• [Presentation] CSF Phosphorylated Tau and Aβ In Seven FAD Cases With Different PSEN1 Mutations (2011)
  • Author(s): Masaki Ikeda, Kimie Yonemura, Satoko Kakuda, Yukio Fujita, Yukiko Hashimoto, Yuichi Tashiro, Koichi Ishiguro, Tsuneo Yamazaki, Masakuni Amari, Yasuo Harigaya, Koichi Okamoto
  • Organizer: AAIC2011
  • Place of Presentation: Paris, France
• [Presentation] プレセニリン1変異AD症例における脳脊髄液のリン酸化タウとAβアミロイドの検討 (2011)
  • Author(s): 池田将樹，角田聡子，藤田行雄 ，橋本由紀子，米村公江，石黒幸一，山崎恒夫，甘利雅邦，針谷康夫，岡本幸市
  • Organizer: 第52回日本神経学会総会
  • Place of Presentation: 名古屋、愛知
• [Presentation] 新規変異プレセニリン1AD症例における脳脊髄液のAβ1-38/1-40/1-42とリン酸化タウとの経時的動的変動の検討 (2011)
  • Author(s): 池田将樹，角田聡子，藤田行雄 ，橋本由紀子，米村公江，石黒幸一，山崎恒夫，甘利雅邦，針谷康夫，岡本幸市
  • Organizer: 第30回日本認知症学会学術集会
  • Place of Presentation: 東京