| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Expanded polyglutamine proteins cause neurodegenerative disorders. Although polyglutamine oligomers have been proposed as a cytotoxic structure, the cytotoxicity of polyglutamine oligomers, not inclusion bodies, has not been proven in human brain tissue. To clarify the cytotoxicity of polyglutamine oligomers, we carried out Semi-Denaturing Detergent-Agarose Gel Electrophoresis (SDD-AGE) and detected oligomers in autopsy brain tissue in DRPLA patients. Quantitative analysis indicates that accumulation of polyglutamine oligomers correlate with the severity of neuronal loss in human brain tissue. These results show that oligomers in human brain play a crucial role in the pathogenesis of polyglutamine diseases.
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