Macrophage function and polymorphism of PKCeta in neuromyelitis optica and multiple sclerosis

• Project/Area Number: 23591247
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Kyushu University
• Principal Investigator: MATSUSHITA Takuya 九州大学, 医学(系)研究科(研究院), 研究員 (00533001)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Satoshi 九州大学, 大学院医学研究院神経内科学, 共同研究員 (20596390)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 多発性硬化症 / 視神経脊髄炎 / マクロファージ / PRKCH

Research Abstract

Polymorphism of PKCeta (rs2230500) was not risk allele or disease modulation factor for multiple sclerosis (MS) or neuromyelitis optica (NMO). However, a ratio of CX3CR1 positive monocytes was increased in rs2230500-A positive group of MS without therapy than in the allele-negative group. From a measurement of cytokines/chemokines of CSF revealed that CXCL8 was increased in rs2230500- A positive group of MS at remission phase and primary progressive MS than in the allele-negative group. These findings suggest differences of function of macrophages or microglia between PKCeta polymorphisms.

Research Products

• [Journal Article] Protein kinase C‐ηpolymorphism rs2230500 does not confer disease susceptibility to multiple sclerosis or neuromyelitis optica (2013)
  • Author(s): Kawano Y, Matsushita T, Cui YW, Isobe N, Yoshimura S, Yonekawa T, Masaki K, Yamasaki R, Murai H, Kira J-I
  • Journal Title: Clin Exp Neuroimmunol Volume: 4(3) Pages: 283-7
• [Journal Article] Alterations in chemokine receptor expressions on peripheral blood monocytes in multiple sclerosis and neuromyelitis optica (2013)
  • Author(s): Cui YW, Kawano Y, Shi N, Masaki K, Isobe N, Yonekawa T, Matsushita T, Tateishi T, Yamasaki R, Murai H
  • Journal Title: Clin Exp Neuroimmunol Volume: 4(2) Pages: 201-5
• [Journal Article] Characteristic cerebrospinal fluid cytokine/chemokine profiles in neuromyelitis optica, relapsing remitting or primary progressive multiple sclerosis (2013)
  • Author(s): Matsushita T, Tateishi T, Isobe N, Yonekawa T, Yamasaki R, Matsuse D, Murai H, Kira J-I
  • Journal Title: PLoS ONE Volume: 8(4)
• [Journal Article] Protein kinase C-η polymorphism rs2230500 does not confer disease susceptibility to multiple sclerosis or neuromyelitis optica (2013)
  • Author(s): Yuji Kawano, Takuya Matsushita, Yi Wen Cui, Noriko Isobe, Satoshi Yoshimura, Tomomi Yonekawa, Katsuhisa Masaki, Ryo Yamasaki, Hiroyuki Murai , Jun-ichi Kira
  • Journal Title: Clinical and Experimental Neuroimmunology Volume: 4 Issue: 3 Pages: 283-287
  • DOI: 10.1111/cen3.12056
  • Peer Reviewed
• [Journal Article] Alterations in chemokine receptor expressions on peripheral blood monocytes in multiple sclerosis and neuromyelitis optica (2013)
  • Author(s): Yi Wen Cui, Yuji Kawano, Nan Shi, Katsuhisa Masaki, Noriko Isobe, Tomomi Yonekawa, Takuya Matsushita, Takahisa Tateishi, Ryo Yamasaki, Hiroyuki Murai, Jun-ichi Kira
  • Journal Title: Clinical and Experimental Neuroimmunology Volume: 4 Issue: 2 Pages: 201-205
  • DOI: 10.1111/cen3.12039
  • Peer Reviewed
• [Journal Article] Distinct genetic and infectious profiles in Japanese neuromyelitis optica patients according to anti-aquaporin-4 antibody status (2013)
  • Author(s): Yoshimura S*, Isobe N*, Matsushita T, Yonekawa T, Masaki K, Sato S, Kawano Y, Kira J
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: 84 Issue: 1 Pages: 29-34
  • DOI: 10.1136/jnnp-2012-302925
  • Peer Reviewed
• [Journal Article] Chharacteristic cerebrospinal fluid cytokine/chemokine profiles in neuromyelitis optica, relapsing remitting or primary progressive multiple sclerosis (2013)
  • Author(s): Matsushita T, Tateishi T, Isobe N, Yonekawa T, Yamasaki R, Murai H, Kira J
  • Journal Title: PLoS ONE Volume: in press
  • Peer Reviewed
• [Journal Article] Comparison of clinical, immunological and neuroimaging features between anti-aquaporin-4 antibody-positive and -negative Sjogren's syndrome patients with central nervous system manifestations (2012)
  • Author(s): Riwanti Estiasari, Matsushita T, Masaki K, Akiyama T, Yonekawa T, Isobe N, Kira J
  • Journal Title: Multiple Sclerosis Volume: 18 Issue: 6 Pages: 807-816
  • DOI: 10.1177/1352458511431727
  • Peer Reviewed
• [Journal Article] Quantitative assays for anti-aquaporin-4 antibody with subclass analysis in neuromyelitis optica (2012)
  • Author(s): Isobe N, Yonekawa T, Matsushita T, Kawano Y, Masaki K, Yoshimura S, Fichna J, Chen S, Furmaniak J, Smith BR, Kira J
  • Journal Title: Multiple Sclerosis Volume: 18 Issue: 11 Pages: 1541-1551
  • DOI: 10.1177/1352458512443917
  • Peer Reviewed
• [Journal Article] Extensive loss of connexins in Balo's disease: evidence for an auto- antibody- independent astrocytopathy via impaired astrocyte-oligodendrocyte/myelin interaction (2012)
  • Author(s): Masaki K, Suzuki SO, Matsushita T, Yonekawa T, Matsuoka T, Isobe N, Motomura K, Wu XM, Tabira T, Iwaki T, Kira J
  • Journal Title: Acta Neuropathol Volume: 123 Issue: 6 Pages: 887-900
  • DOI: 10.1007/s00401-012-0972-x
  • Peer Reviewed
• [Journal Article] Genetic and infectious profiles of Japanese multiple sclerosis patients (2012)
  • Author(s): Yoshimura S, Isobe N, Yonekawa T, Matsushita T, Masaki K, Sato S, Kawano Y, Yamamoto K, Kira J
  • Journal Title: PLoS ONE Volume: 7 Issue: 11 Pages: e48592-e48592
  • DOI: 10.1371/journal.pone.0048592
  • Peer Reviewed
• [Presentation] Cytokine receptor expressions in monocyte subsets in multiple sclerosis, neuromyelitis optica, and amyotrophic lateral sclerosis (2013)
  • Author(s): Cui YW, Kawano Y, Shi N, Masaki K, Isobe N, Yonekawa T, Matsushita T, Tateishi T, Yamasaki R, Kira J
  • Organizer: PACTRIMS
  • Place of Presentation: Kyoto
• [Presentation] Dysregulation of surface marker expressions on peripheral monocytes in multiple sclerosis (2012)
  • Author(s): Cui YW、Masaki K、Yamasaki R、Tateishi T、Matsushita T、Shi N、Ohyagi Y、Kira J
  • Organizer: PACTRIMS
  • Place of Presentation: Beijing
• [Presentation] 日本人多発性硬化症の遺伝的要因と環境要因の相互作用 (2012)
  • Author(s): 吉村怜、磯部紀子、松下拓也、米川智、眞崎勝久、佐藤眞也、吉良潤一
  • Organizer: 第109回日本内科学会講演会
  • Place of Presentation: 京都
• [Presentation] Distinct genetic and infectious profiles between multiple sclerosis and neuromyelitis optica in Japanese patients (2012)
  • Author(s): Yoshimura S、Yonekawa T、Isobe N、Masaki K、Sato S、Matsushita T、Kira J
  • Organizer: American Academy of Neurology
  • Place of Presentation: New Orleans
• [Presentation] 日本人脱髄性疾患に影響する遺伝的要因と環境中の普遍的な感染性要因の同定 (2012)
  • Author(s): 吉村怜、磯部紀子、米川智、佐藤眞也、真崎勝久、松下拓也、吉良潤一
  • Organizer: 第53回日本神経学会学術大会
  • Place of Presentation: 東京
• [Presentation] 日本人多発性硬化症 (MS) 患者でも髄液IgG産生異常は、 HLA-DRB1*1501アリルとBarkhof基準を満たす脳MRI病巣に有意に相関する (2012)
  • Author(s): 吉村怜、磯部紀子、米川智、佐藤眞也、真崎勝久、松下拓也、吉良潤一
  • Organizer: 第24回日本神経免疫学会学術集会
  • Place of Presentation: 長野
• [Presentation] 日本人脱髄性疾患の疾患関連遺伝子 (2012)
  • Author(s): 吉村怜、磯部紀子、松下拓也、吉良潤一
  • Organizer: 第40回日本臨床免疫学会総会
  • Place of Presentation: 東京
• [Presentation] 視神経脊髄炎，多発性硬化症，Baló病におけるアストロサイト障害の特徴 (2011)
  • Author(s): 松下拓也
  • Organizer: 第52回日本神経学会学術大会(招待講演)
  • Place of Presentation: 名古屋
• [Presentation] 抗aquaporin-4（AQP4）抗体を有する中枢神経障害合併シェーグレン症候群の特徴 (2011)
  • Author(s): 松下拓也， Riwanti Estiasari，真崎勝久， 米川智，吉良潤一
  • Organizer: 第52回日本神経学会学術大会
  • Place of Presentation: 名古屋
• [Presentation] サイトカイン・ケモカインプロフィールからみた視神経脊髄炎・多発性硬化症の特徴 (2011)
  • Author(s): 松下拓也
  • Organizer: 第23回日本神経免疫学会学術集会(招待講演)
  • Place of Presentation: 東京
• [Presentation] 視神経脊髄炎における抗アクアポリン4抗体によるHLAクラスIIアリル頻度の差異および多発性硬化症との比較 (2011)
  • Author(s): 松下拓也，吉村怜，磯部紀子，米川智，眞崎勝久，吉良潤一，the South Japan Multiple Sclerosis Genetics Consortium
  • Organizer: 第23回日本神経免疫学会学術集会
  • Place of Presentation: 東京
• [Presentation] 多発性硬化症・視神経脊髄炎におけるサイトカイン/ケモカイン動態 (2011)
  • Author(s): 松下拓也，立石貴久，米川智，磯部紀子，吉良潤一
  • Organizer: 第23回日本神経免疫学会学術集会
  • Place of Presentation: 東京
• [Presentation] Increased CSF IL-17A, IL-6, CXCL8 and CXCL10 in patients with neuromyelitis optica at relapse and their clinical relevance (2011)
  • Author(s): Takuya Matsushita, Takahisa Tateishi, Tomomi Yonekawa, Noriko Isobe, Jun-ichi Kira
  • Organizer: 5TH JOINT TRIENNIAL CONGRESS of ECTRIMS/ACTRIMS
  • Place of Presentation: Amsterdam
• [Book] MS初の経口治療薬．フィンゴリモドの薬理作用と臨床成績． (多発性硬化症と視神経脊髄炎の基礎と臨床) (2012)
  • Author(s): 吉村怜、吉良潤一
  • Total Pages: 264
  • Publisher: 医薬ジャーナル