| Project/Area Number |
23591251
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | Kyushu University of Nursing and Social Welfare (2013)
Oita University (2011-2012) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KIMURA Noriyuki 大分大学, 医学部, 講師 (30433048)
NAKAMURA Kenichiro 大分大学, 医学部, 助教 (70608372)
MASUDA Teruaki 大分大学, 医学部, 助教 (50464459)
OKAZAKI Toshio 大分大学, 医学部, 助教 (00464438)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 神経分子病態学 / 遠位型ミオパチー / 縁取り空胞 / GNE / オートファジー / リソソーム / プロテアソーム / 小胞体ストレス / プロテアゾーム / valosin/p97 |
|---|
| Research Abstract |
In mutant GNE gene-transfected cells and GNE-knockdown cells, the excessive formation of rimmed vacuole and the morphological change of an autophagy-lysosome system structures were not observed, but the level of mTOR phosphorylation increased, suggesting that GNE play a certain role in regulation of an autophagy-lysosome system through mTOR. The VCP/p97-Nfd1-Npl4 complex is suspected to participate in the overdevelopment formation of rimmed vacuoles. IGF-1 or salubrinal treatment may suppress the overdevelopment of rimmed vacuoles due to activation (phosphorylation) of mTOR and/or elF2.
|
