| Project/Area Number |
23591281
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Keio University |
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Principal Investigator |
ITOH Yoshiaki 慶應義塾大学, 医学部, 講師 (90265786)
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| Co-Investigator(Kenkyū-buntansha) |
UNEKAWA Miyuki 慶應義塾大学, 医学部, 特任助教 (10548481)
HOSHINO Haruhiko 慶應義塾大学, 医学部, 講師(非常勤) (50407110)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2012: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | 人工血管 / 脳虚血治療 / 血管新生 / 内皮細胞 / 治療的血管新生 / 人工血管シート / 脳血管内皮細胞 / 主幹動脈閉塞症 / 再生医療 / 移植医療 / 血管再生 / 血管内皮細胞 / 脳虚血 / 周皮細胞 / 治療 |
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| Research Abstract |
Background: Therapeutic angiogenesis is now actively studied as a method to aleviate cerebral infarction after major artery occlusion. We developed a tissue engineered blood vessel sheet to facilitate bypass flow in an animal model of arterial occlusion.
Methods: Human brain micirovessel endothelial cells were labeled with GFP and were seeded in collagen membrane together with 10T1/2 cells, mesenchymal precurser cells. After craniectomy in immunosuppressed mouse (SCID mouse), the membrane with endothelial cells were placed over the cortex and pia matter.Results: Immature vessel structure was observed after 3 days and blood flow from cortical arteriole was observed after 5 days. Size of cerebral infarct induced by permanent MCA occlusion became much smaller than control.
Conclusion: Tissue engineered pial vessel may be useful in prevention of cerebral infarction after major artery occlusion.
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