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Tissue engineered blood vessel sheet to prevent cerebral infarction

Research Project

Project/Area Number 23591281
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurology
Research InstitutionKeio University

Principal Investigator

ITOH Yoshiaki  慶應義塾大学, 医学部, 講師 (90265786)

Co-Investigator(Kenkyū-buntansha) UNEKAWA Miyuki  慶應義塾大学, 医学部, 特任助教 (10548481)
HOSHINO Haruhiko  慶應義塾大学, 医学部, 講師(非常勤) (50407110)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2012: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords人工血管 / 脳虚血治療 / 血管新生 / 内皮細胞 / 治療的血管新生 / 人工血管シート / 脳血管内皮細胞 / 主幹動脈閉塞症 / 再生医療 / 移植医療 / 血管再生 / 血管内皮細胞 / 脳虚血 / 周皮細胞 / 治療
Research Abstract

Background: Therapeutic angiogenesis is now actively studied as a method to aleviate cerebral infarction after major artery occlusion. We developed a tissue engineered blood vessel sheet to facilitate bypass flow in an animal model of arterial occlusion.
Methods: Human brain micirovessel endothelial cells were labeled with GFP and were seeded in collagen membrane together with 10T1/2 cells, mesenchymal precurser cells. After craniectomy in immunosuppressed mouse (SCID mouse), the membrane with endothelial cells were placed over the cortex and pia matter.Results: Immature vessel structure was observed after 3 days and blood flow from cortical arteriole was observed after 5 days. Size of cerebral infarct induced by permanent MCA occlusion became much smaller than control.
Conclusion: Tissue engineered pial vessel may be useful in prevention of cerebral infarction after major artery occlusion.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (29 results)

All 2014 2013 2012 2011 Other

All Journal Article (14 results) (of which Peer Reviewed: 11 results,  Open Access: 1 results) Presentation (12 results) (of which Invited: 2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] 34. Microvascular sprouting, extension, and creation of new capillary connection with adaptation of the neighboring astrocytes in adult mouse cortex under chronic hypoxia2014

    • Author(s)
      Masamoto K, Takuwa H, Seki C, Taniguchi J, Itoh Y, Tomita Y, Toriumi H, Unekawa M, Kawaguchi H, Ito H, Suzuki N, Kanno I
    • Journal Title

      J Cereb Blood Flow Metab

      Volume: 34 Issue: 2 Pages: 325-31

    • DOI

      10.1038/jcbfm.2013.201

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 脳梗塞急性期の抗凝固療法・抗血小板療法2013

    • Author(s)
      伊藤義彰
    • Journal Title

      臨床神経

      Volume: 53 Pages: 1172-1174

    • NAID

      130004505375

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse2013

    • Author(s)
      Sekiguchi Y, Masamoto K, Takuwa H, Kawaguchi H, Kanno I, Ito H, Tomita Y, Itoh Y, Suzuki N, Sudo R, Tanishita K
    • Journal Title

      Advances in experimental medicine and biology

      Volume: 789 Pages: 419-25

    • DOI

      10.1007/978-1-4614-7411-1_56

    • ISBN
      9781461472568, 9781461474111
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and clinical features of asymptomatic peripheral artery disease in Japanese stroke patients.2013

    • Author(s)
      Hoshino H, Itoh Y, Yamada S, Suzuki N
    • Journal Title

      J Stroke Cerebrovasc Dis

      Volume: 22 Issue: 3 Pages: 255-9

    • DOI

      10.1016/j.jstrokecerebrovasdis.2011.08.011

    • Related Report
      2013 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cilostazol suppresses leukocyte rolling and adhesion on cerebral microvessels after ischemia/reperfusion2012

    • Author(s)
      Itoh Y, Toriumi H, Yamada S, Konoeda F, Suzuki N
    • Journal Title

      Microvascular Reviews and Communications

      Volume: 5(1) Pages: 2-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 虚血組織に対する治療的血管新生2012

    • Author(s)
      伊藤義彰、黒井俊哉、鳥海春樹、海老根妙子、畝川美悠紀、山田哲、此枝史恵、鈴木則宏
    • Journal Title

      脳循環代謝

      Volume: 23 Pages: 90-94

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Control of brain capillary blood flow2012

    • Author(s)
      Itoh Y, Suzuki N.
    • Journal Title

      J Cerebral Blood Flow Met

      Volume: 32 Issue: 7 Pages: 1167-76

    • DOI

      10.1038/jcbfm.2012.5

    • Related Report
      2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Repeated longitudinal in vivo imaging of neuro-glio-vascular unit at the peripheral boundary of ischemia inmouse cerebral cortex2012

    • Author(s)
      Masamoto K, TomitaY, Toriumi H, Aoki I, Unekawa M, Takuwa H, Itoh Y, Suzuki N, Kanno I
    • Journal Title

      Neuroscience

      Volume: (未)(Epub ahead of print) Pages: 190-200

    • DOI

      10.1016/j.neuroscience.2012.03.034

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cilostazol suppresses leukocyte rolling and adhesion on cerebral microvessels after ischemia/reperfusion.2012

    • Author(s)
      Itoh Y, Toriumi H, Yamada S, Konoeda F, Suzuki N.
    • Journal Title

      Microvascular Reviews and Communications

      Volume: 5 Pages: 2-8

    • Related Report
      2012 Research-status Report
  • [Journal Article] Burden of overactive bladder symptom on quality of life in stroke patients2012

    • Author(s)
      Itoh Y, Yamada S, Konoeda F, Koizumi K, Nagata H, Oya M, Suzuki N.
    • Journal Title

      Neurourol Urodyn

      Volume: Epub ahead of print Issue: 5 Pages: 428-434

    • DOI

      10.1002/nau.22336

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Capillary remodeling and collateral growth without angiogenesis after unilateral common carotid artery occlusion in mice2011

    • Author(s)
      Guo H, Itoh Y, Toriumi H, Yamada S, Tomita Y, Hoshino H, Suzuki N
    • Journal Title

      Microcirculation

      Volume: 18(3) Pages: 221-7

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Astrocytes and pericytes cooperatively maintain a capillary-like structure composed of endothelial cells on gel matrix2011

    • Author(s)
      Itoh Y, Toriumi H, Yamada S, Hoshino H, Suzuki N
    • Journal Title

      Brain Res

      Volume: 1406 Pages: 74-83

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Astrocytes and pericytes cooperatively maintain a capillary-like structure composed of endothelial cells on gel matrix.2011

    • Author(s)
      Itoh Y, Toriumi H, Yamada S, Hoshino H, Suzuki N.
    • Journal Title

      Brain Res.

      Volume: 1406 Pages: 74-83

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Capillary remodeling and collateral growth without angiogenesis after unilateral common carotid artery occlusion in mice.2011

    • Author(s)
      Guo H, Itoh Y, Toriumi H, Yamada S, Tomita Y, Hoshino H, Suzuki N.
    • Journal Title

      Microcirculation.

      Volume: 18(3) Pages: 221-7

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 脳梗塞急性期の抗凝固療法・抗血小板療法2013

    • Author(s)
      伊藤義彰
    • Organizer
      第54回日本神経学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Irbesartan長期投与による脳虚血再灌流領域の血管への炎症性細胞接着の抑制2013

    • Author(s)
      鳥海春樹, 海老根妙子, 萱間洋平, 畝川美悠紀, 山田哲, 冨田裕, 伊藤義彰, 鈴木則宏
    • Organizer
      脳卒中学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 脳梗塞患者における血管拡張反応による動脈硬化の評価2013

    • Author(s)
      関守信、滝沢翼、山田哲、此枝史恵、伊藤義彰、鈴木則宏
    • Organizer
      脳卒中学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Repeated 3D imaging of microvascular-astroglia restructuring induced by chronic hypoxia in mouse cerebral cortex2013

    • Author(s)
      Kazuto Masamoto, Hiroyuki Takuwa, Takuma Sugashi, Yukio Yamada, Yutaka Tomita, Miyuki Unekawa, Haruki Toriumi, Yoshiaki Itoh, Norihiro Suzuki, Hiroshi Ito, Iwao Kanno
    • Organizer
      日本微小循環学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Propagation of changes in diameter of pial arteries and cerebral blood flow following cortical spreading depression in anesthetized mice2013

    • Author(s)
      Miyuki Unekawa, Yutaka Tomita, Haruki Toriumi, Takashi Osada, Kazuto Masamoto, Yoshiaki Itoh, Iwao Kanno, Norihiro Suzuki
    • Organizer
      日本微小循環学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 脳小血管病におけるneurovascular unitの障害2012

    • Author(s)
      伊藤義彰
    • Organizer
      第53回日本神経学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2012-05-23
    • Related Report
      2013 Final Research Report
  • [Presentation] 脳小血管病におけるNeurovascular Unitの障害2012

    • Author(s)
      伊藤義彰、鳥海春樹、畝川美悠紀、海老根妙子、山田哲、此枝史恵、小泉健三、冨田裕、鈴木則宏
    • Organizer
      第53回日本神経学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 虚血組織に対する治療的血管新生2011

    • Author(s)
      伊藤義彰
    • Organizer
      第23回日本脳循環代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2011-11-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 虚血組織に対する治療的血管新生

    • Author(s)
      伊藤義彰
    • Organizer
      第23回脳循環代謝学会総会(招待講演)
    • Place of Presentation
      都市センターホテル、東京都
    • Related Report
      2011 Research-status Report
  • [Presentation] Changes in diameter of cerebral microvessels and blood flow associated with potassium-induced cortical spreading depression in anesthetized mice

    • Author(s)
      Miyuki Unekawa, Yutaka Tomita, Haruki Toriumi, Taeko Ebine, Kazuto Masamoto, Yoshiaki Itoh, Iwao Kanno, Norihiro Suzuki
    • Organizer
      日本微小循環学会
    • Place of Presentation
      盛岡グランドホテル、岩手県
    • Related Report
      2011 Research-status Report
  • [Presentation] Resident Endothelial Cells Surrounding Damaged Arterial Endothelium Reendothelialize the Lesion without Any Involvement of Foreign Progenitor Cells

    • Author(s)
      Y. Itoh, H. Toriumi, S. Yamada, Y. Tomita, H. Hoshino, N. Suzuki
    • Organizer
      XXVth International Symposium on Cerebral Blood Flow
    • Place of Presentation
      バルセロナ、スペイン
    • Related Report
      2011 Research-status Report
  • [Presentation] マウス一側総頸動脈閉塞後に観察された血管新生を伴わない毛細血管の動脈化と側副血行路の発達

    • Author(s)
      伊藤義彰、郭淮莲、鳥海春樹、山田哲、小泉健三、冨田裕
    • Organizer
      日本脳卒中学会
    • Place of Presentation
      京都国際会議場、京都府
    • Related Report
      2011 Research-status Report
  • [Book] 「炎症性プラークのイメージング」Annual Review神経2013

    • Author(s)
      伊藤義彰
    • Publisher
      中外医学社
    • Related Report
      2013 Final Research Report
  • [Book] 脳卒中診療こんなときどうするQ&A第二版2011

    • Author(s)
      伊藤義彰
    • Publisher
      中外医学社
    • Related Report
      2013 Final Research Report
  • [Remarks] 慶應義塾大学医学部神経内科

    • URL

      http://www.keio-med.jp/neurology/index.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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