Tissue engineered blood vessel sheet to prevent cerebral infarction
Project/Area Number |
23591281
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurology
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Research Institution | Keio University |
Principal Investigator |
ITOH Yoshiaki 慶應義塾大学, 医学部, 講師 (90265786)
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Co-Investigator(Kenkyū-buntansha) |
UNEKAWA Miyuki 慶應義塾大学, 医学部, 特任助教 (10548481)
HOSHINO Haruhiko 慶應義塾大学, 医学部, 講師(非常勤) (50407110)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2012: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
|
Keywords | 人工血管 / 脳虚血治療 / 血管新生 / 内皮細胞 / 治療的血管新生 / 人工血管シート / 脳血管内皮細胞 / 主幹動脈閉塞症 / 再生医療 / 移植医療 / 血管再生 / 血管内皮細胞 / 脳虚血 / 周皮細胞 / 治療 |
Research Abstract |
Background: Therapeutic angiogenesis is now actively studied as a method to aleviate cerebral infarction after major artery occlusion. We developed a tissue engineered blood vessel sheet to facilitate bypass flow in an animal model of arterial occlusion. Methods: Human brain micirovessel endothelial cells were labeled with GFP and were seeded in collagen membrane together with 10T1/2 cells, mesenchymal precurser cells. After craniectomy in immunosuppressed mouse (SCID mouse), the membrane with endothelial cells were placed over the cortex and pia matter.Results: Immature vessel structure was observed after 3 days and blood flow from cortical arteriole was observed after 5 days. Size of cerebral infarct induced by permanent MCA occlusion became much smaller than control. Conclusion: Tissue engineered pial vessel may be useful in prevention of cerebral infarction after major artery occlusion.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] 34. Microvascular sprouting, extension, and creation of new capillary connection with adaptation of the neighboring astrocytes in adult mouse cortex under chronic hypoxia2014
Author(s)
Masamoto K, Takuwa H, Seki C, Taniguchi J, Itoh Y, Tomita Y, Toriumi H, Unekawa M, Kawaguchi H, Ito H, Suzuki N, Kanno I
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Journal Title
J Cereb Blood Flow Metab
Volume: 34
Issue: 2
Pages: 325-31
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Measuring the vascular diameter of brain surface and parenchymal arteries in awake mouse2013
Author(s)
Sekiguchi Y, Masamoto K, Takuwa H, Kawaguchi H, Kanno I, Ito H, Tomita Y, Itoh Y, Suzuki N, Sudo R, Tanishita K
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Journal Title
Advances in experimental medicine and biology
Volume: 789
Pages: 419-25
DOI
ISBN
9781461472568, 9781461474111
Related Report
Peer Reviewed
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[Presentation] Repeated 3D imaging of microvascular-astroglia restructuring induced by chronic hypoxia in mouse cerebral cortex2013
Author(s)
Kazuto Masamoto, Hiroyuki Takuwa, Takuma Sugashi, Yukio Yamada, Yutaka Tomita, Miyuki Unekawa, Haruki Toriumi, Yoshiaki Itoh, Norihiro Suzuki, Hiroshi Ito, Iwao Kanno
Organizer
日本微小循環学会
Place of Presentation
東京
Related Report
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