| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
In the current study, we explored the intracellular signaling pathway of a hypothalamic anorexic hormone, Nesfatin-1 (NAP-1). Radioreceptor assays indicated that NAP-1 specific receptor should be located in the cell membrane fraction of mouse hypothalamus and of NB41A3 cells, which derived from mouse neuroblastoma. Through MAP kinase and L-type calcium channel, NAP-1 increased intracellular phosphorylation of cAMP response element binding protein (CREB). Moreover, it was revealed that in pancreatic beta cells, NAP-1 employed a distinct signaling pathway from that in central nervous system. We identified a candidate gene (GPCRn) for NAP-1 specific receptor and established GPCRn deficient mice (GPCRnKO).
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