Molecular mechanism of intracellular and in vivo function of an anorexic peptide, Nesfatin-1
Project/Area Number |
23591296
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Metabolomics
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Research Institution | Tokyo Medical and Dental University (2013) Gunma University (2011-2012) |
Principal Investigator |
HASHIMOTO Koshi 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (30396642)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | Nesfatin-1 / Nesfatin-1受容体 / 摂食抑制ペプチド / 細胞内シグナル伝達 / CREB / 膵β細胞 / 視床下部 / M30 / Nap-1 / Nap-1受容体 / [35S]GTPγS アッセイ / 摂食抑制 / 遺伝子 / 細胞内シグナル伝達機構 / リン酸化CRE 結合蛋白(p-CREB) |
Research Abstract |
In the current study, we explored the intracellular signaling pathway of a hypothalamic anorexic hormone, Nesfatin-1 (NAP-1). Radioreceptor assays indicated that NAP-1 specific receptor should be located in the cell membrane fraction of mouse hypothalamus and of NB41A3 cells, which derived from mouse neuroblastoma. Through MAP kinase and L-type calcium channel, NAP-1 increased intracellular phosphorylation of cAMP response element binding protein (CREB). Moreover, it was revealed that in pancreatic beta cells, NAP-1 employed a distinct signaling pathway from that in central nervous system. We identified a candidate gene (GPCRn) for NAP-1 specific receptor and established GPCRn deficient mice (GPCRnKO).
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Report
(4 results)
Research Products
(19 results)
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[Presentation] 摂食抑制ペプチドnesfatin-1の細胞内シグナル伝達機構の解析とその特異的受容体の探索2013
Author(s)
橋本貢士,石田恵美,武田茂樹,登丸琢也,石井角保,小澤厚志,渋沢信行,佐藤哲郎,岡田秀一,清水弘行,山田正信,森昌朋
Organizer
第86回日本内分泌学会学術総会
Place of Presentation
仙台国際センター(宮城県)
Related Report
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[Presentation] 摂食抑制ペプチドnesfatin-1の細胞内シグナル伝達機構の解析とその特異的受容体の探索2013
Author(s)
橋本貢士, 石田 恵美, 武田茂樹, 登丸琢也, 石井角保, 小澤厚志, 渋沢信行, 佐藤哲郎, 岡田秀一, 清水 弘行, 山田 正信, 森 昌朋
Organizer
第86回日本内分泌学会学術総会
Place of Presentation
仙台国際センター
Related Report
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