Role of Akt1 and Akt2 in Cardiac and Skeletal Muscle

• Project/Area Number: 23591299
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: The University of Tokyo
• Principal Investigator: OKAZAKI Yukiko 東京大学, 医学部附属病院, 助教 (30422299)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 骨格筋 / 心筋

Research Abstract

In this study, to gain insight into the role of Akt in heart and SKM, we ablated Akt1 and Akt2 by crossing Akt1 and Akt2 floxed mice with MCK-Cre or MLC1-Cre transgenic mice, respectively, resulting in both heart and SKM specific Akt1/Akt2 knockout (M-Akt1/2DKO)mice or SKM specific Akt1/Akt2 knockout (MLC1-Akt1/2DKO) mice. M-Akt1/2DKO mice died within 5 weeks after birth due to heart failure. Quantitative RT-PCR analysis revealed genes regulating oxidative phosphorylation were decreased significantly in the heart of M-Akt1/2DKO mice. In MLC1-Akt1/2DKO mice, the body weight and skeletal muscle volume were decreased at 6 weeks after birth, and showed impaired glucose tolerance and insulin resistance at 32 weeks.
These data suggest that Akt1 and Akt2 play an important role for maintaining heart and SKM growth and function by regulating mitochondrial energy metabolism and controlling quantity and quality of skeletal muscle which are involved in glucose metabolism in whole body.

Research Products

• [Presentation] PA-061心筋・骨格筋におけるAkt1およびAkt2の機能の解明 (2011)
  • Author(s): 梅原敏弘、植木浩二郎、鈴木美穂、金子和真、岩根亜弥、粟澤元晴、笹子敬洋、小林正稔、岡﨑由希子、大杉満、Birnbaum Morris J
  • Organizer: 第11回東京大学生命科学シンポジウムTodai BIO2011
  • Place of Presentation: 東京, 東京大学安田講堂及び工学部2号館
  • Year and Date: 2011-06-04
• [Presentation] Roles of Akt1 and Akt2 in cardiac and skeletal muscle (2011)
  • Author(s): 梅原敏弘、梅原敏弘、植木浩二郎、金子和真、鈴木美穂、岩根亜弥、粟澤元晴、笹子敬洋、小林正稔、岡﨑由希子、大杉満、Birnbaum Morris J、門脇孝
  • Organizer: 第16回日韓糖尿病シンポジウム
  • Place of Presentation: 千葉県浦安市, ヒルトン東京ベイ
• [Presentation] 0-142心筋・骨格筋におけるAkt1およびAkt2の機能の解明 (2011)
  • Author(s): 梅原敏弘、植木浩二郎、金子和真、鈴木美穂、岩根亜弥、粟澤元晴、笹子敬洋、小林正稔、岡﨑由希子、大杉満、Birnbaum Morris、門脇孝
  • Organizer: 第32回日本肥満学会年次学術集会
  • Place of Presentation: 兵庫県淡路市, 淡路夢舞台国際会議場
• [Presentation] Role of Akt1 and Akt2 Cardiac and Skeletal Muscle (2011)
  • Author(s): Yukiko Okazaki, Kohjiro Ueki, Toshihiro Umehara, Kazuma Kaneko, Miho Suzuki, Takayoshi Sasako, Motoharu Awazawa, Mitusru Ohsugi, Morris Birnbaum, Takashi Kadowaki
  • Organizer: 3^<rd> Annual Meeting of the Asian Association for the Study of Diabetes
  • Place of Presentation: Beijing、China
• [Presentation] 血管内皮的特異的Akt/PKBノックアウトマウスの解析 (2011)
  • Author(s): 梅原敏弘、植木浩二郎、鈴木美穂、金子和真、岩根亜弥、阪田道教、笹子敬洋、小林正稔、粟澤元晴、岡﨑由希子、大杉満、Birnbaum Morris J.、門脇孝
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌, さっぽろ文化の館他
• [Presentation] 心筋・骨格筋におけるAkt1/Akt2の役割の解明 (2011)
  • Author(s): 金子和真、植木浩二郎、鈴木美穂、梅原敏弘、粟澤元晴、笹子敬洋、小林正稔、岡﨑由希子、大杉満、門脇孝
  • Organizer: 第84回日本内分泌学会学術集会
  • Place of Presentation: 神戸, 神戸国際会議場