Effects of transplantation of neural crest cells derived from iPS cells on diabetic polyneuropathy in mice
Project/Area Number |
23591303
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Metabolomics
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Research Institution | Aichi Medical University |
Principal Investigator |
NAKAMURA Jiro 愛知医科大学, 医学部, 教授 (40283444)
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Co-Investigator(Kenkyū-buntansha) |
ISOBE Kennichi 名古屋大学, 医学系研究科, 教授 (20151441)
KAMIYA Hideki 愛知医科大学, 医学部, 准教授 (70542679)
NARUSE Keiko 愛知学院大学, 歯学部, 准教授 (30387576)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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Keywords | 糖尿病性多発神経障害 / 細胞移植 / 人工多能性幹細胞 / 神経堤細胞 / 糖尿病性神経障害 / 再生医療 / iPS細胞 |
Research Abstract |
Recent studies have shown that transplantation of neural crest cells (NCCs) is effective on spinal cord injury. Here we used NCCs from aged mouse iPS cells for treatment of diabetic polyneuropathy. iPS cells were differentiated on PA6 cells with BMP4 , and p75 positive cells were defined as NCCs. NCCs were transplanted into hind limb skeletal muscles of STZ-diabetic mice. Transplanted NCCs differentiated into vascular smooth muscle cells or Schwann cells in plantar skin or soleus muscle. Impaired nerve functions in diabetic mice were ameliorated by the transplantation of NCCs. In addition, transplantation of NCCs increased intraepidermal nerve fiber densities, plantar skin blood flow and capillaries in DM. These results suggest that transplantation of NCCs derived from iPS cells could have therapeutic effects on DPN through paracrine actions of growth factors secreted by NCCs and differentiation of NCCs into Schwann cells and vascular smooth muscle cells.
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Report
(4 results)
Research Products
(59 results)
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[Journal Article] The role of S100B in the interaction between adipocytes and macrophages.2014
Author(s)
Fujiya A, Nagasaki H, Seino Y, Okawa T, Kato J, Fukami A, Uenishi E, Tsunekawa S, Kamiya H, Nakamura J, Oiso Y, Hamada Y.
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Journal Title
Obesity
Volume: 22
Pages: 371-379
Related Report
Peer Reviewed
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[Journal Article] Sensory and motor physiological functions are impaired in gastric inhibitory polypeptide receptor-deficient mice.2014
Author(s)
Okawa T, Kamiya H, Himeno T, Seino Y, Tsunekawa S, Hayashi Y, Harada N, Yamada Y, Inagaki N, Seino Y, Oiso Y, Nakamura J.
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Journal Title
J Diabetes Invest
Volume: 5
Pages: 31-37
Related Report
Peer Reviewed
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[Journal Article] Transplantation of neural crest like cells derived from induced pluripotent stem cells improves diabetic polyneuropathy in mice2013
Author(s)
Okawa T, Kamiya H, Himeno T, Kato J, Seino Y, Fujiya A, Kondo M, Tsunekawa S, Naruse K, Hamada Y, Ozaki N, Cheng Z, Kito T, Suzuki H, Ito S, Nakamura J, Isobe K
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Journal Title
Cell Transplant
Volume: 22
Pages: 1763-1783
Related Report
Peer Reviewed
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[Journal Article] Mesenchymal stem cell-like cells derived from mouse induced pluripotent stem cells ameliorate diabetic polyneuropathy in mice.2013
Author(s)
Himeno T, Kamiya H, Naruse K, Cheng Z, Ito S, Kondo M, Okawa T, Fujiya A, Kato J, Suzuki H, Kito T, Hamada Y, Oiso Y, Isobe K, Nakamura J.
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Journal Title
Biomed Res Int
Volume: -
Pages: 259187-259187
DOI
Related Report
Peer Reviewed
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[Journal Article] Transplantation of neural crest like cells derived from induced pluripotent stem cells improves diabetic polyneuropathy in mice.2013
Author(s)
Okawa T, Kamiya H, Himeno T, Kato J, Seino Y, Fujiya A, Kondo M, Tsunekawa S, Naruse K, Hamada Y, Ozaki N, Cheng Z, Kito T, Suzuki H, Ito S, Nakamura J, Isobe K
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Journal Title
Cell Transplant
Volume: 22
Pages: 1763-1783
Related Report
Peer Reviewed
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[Journal Article] Beneficial effects of exendin-4 on experimental polyneuropathy in diabetic mice2011
Author(s)
Himeno T, Kamiya H, Naruse K, Ozaki N, Seino Y, Shibata T, Kondo M, Kato J, Okawa T, Fukami A, Hamada Y, Inagaki N, Seino Y, Drucker D. J, Oiso Y, Nakamura J
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Journal Title
Diabetes
Volume: 60
Pages: 2387-2406
NAID
Related Report
Peer Reviewed
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[Journal Article] Transplantation of bone marrow-derived mononuclear cells improves mechanical hyperalgesia, cold allodynia and nerve function in diabetic neuropathy2011
Author(s)
Naruse K, Sato J, Funakubo M, Hata M, Nakamura N, Kobayashi Y, Kamiya H, Shibata T, Kondo M, Himeno T, Matsubara T, Oiso Y, Nakamura J
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Related Report
Peer Reviewed
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[Presentation] Transplantation of Induced neural crest cells from spheroidal dermal stem cells improves diabetic polyneuropathy in mice2013
Author(s)
Kondo M, Kamiya H, Himeno T, Kato J, Okawa T, Kato J, Fujiya A, Hamada Y, Naruse K, Nishio N, Ito S, Oiso Y, Isobe K, Nakamura J
Organizer
The 73th the American Diabetes Association Scientific Sessions
Place of Presentation
Chicago, USA
Related Report
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[Presentation] Transplantation of neural progenitor-like cells derived from spheroidal dermal cells improves diabetic polyneuropathy in mice2012
Author(s)
Kondo M, Kamiya H, Himeno T, Kato J, Okawa T, Fujiya A, Naruse K, Hamada Y, Nishio N, Ito S, Oiso Y, Isobe K, Nakamura J.
Organizer
The 72th the American Diabetes Association Scientific Sessions
Place of Presentation
Philadelphia, USA
Related Report
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[Presentation] Mesenchymal stem cells improve impaired foot ulcer healing in diabetic rats2012
Author(s)
Kato J, Kamiya H, Himeno T, Shibata T, Okawa T, Kondo M, Fujiya A, Seino Y, Tsunekawa S, Hamada Y, Naruse K, Oiso Y, Nakamura J
Organizer
The 72th the American Diabetes Association Scientific Sessions
Place of Presentation
Philadelphia, USA
Related Report
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[Presentation] The Function of glucose-responsive ATP-Sensitive K (KATP) channels in peripheral nervous systems.2012
Author(s)
Himeno T, Kamiya H, Seino Y, Naruse K, Okawa T, Kato J, Kondo M, Fujiya A, Uenishi E, Tsunekawa S, Hamada Y, Oiso Y, Seino S, Nakamura J.
Organizer
The 72th the American Diabetes Association Scientific Sessions
Place of Presentation
Philadelphia, USA
Related Report
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[Presentation] The function of gastric inhibitory polypeptide signals in peripheral nervous systems in mice2012
Author(s)
Okawa T, Kamiya H, Himeno T, Harada N, Kato J, Naruse K, Fujiya A, Seino Y, Fukami A, Tsunekawa S, Hayashi Y, Hamada Y, Yamada Y, Inagaki N, Seino Y, Oiso Y, Nakamura J.
Organizer
The 72th the American Diabetes Association Scientific Sessions
Place of Presentation
Philadelphia, USA
Related Report
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