| Project/Area Number |
23591305
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
FUJIKURA Junji 京都大学, 医学(系)研究科(研究院), 助教 (70378743)
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| Co-Investigator(Renkei-kenkyūsha) |
ASAKA Isao 京都大学, iPS細胞研究所, 准教授 (10543639)
ADACHI Soichi 京都大学, 医学研究科, 教授 (10273450)
TAKAHASHI Kazutoshi 京都大学, iPS細胞研究所, 講師 (80432326)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 再生医療 / 糖尿病 / 人工多能性幹細胞 / ミトコンドリア / iPS細胞 |
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| Research Abstract |
iPS cells were successfully generated from patients with the mtDNA A3243G mutation(Mt-iPS).The Mt-iPS cells exhibited a bimodal degree of mutation heteroplasmy. The mutation frequencies decreased to an undetectable level in six of 14 clones, while the others showed several-fold increases in mutation frequencies compared with those in the original fibroblasts. During serial cell culture passage and after differentiation, no recurrence of the mutation or no significant changes in the levels of heteroplasmy were seen. Mutation-rich, stable Mt-iPS cells may be a suitable source of cell for human mitochondrial disease modelling in vitro. Mutation-free iPS cells could provide an unlimited, disease free supply of cells for autologous transplantation therapy.
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