Toward novel molecular targeting strategies for the metabolic syndrome

• Project/Area Number: 23591318
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KISHIDA Tsunao 京都府立医科大学, 医学(系)研究科(研究院), 講師 (00370205)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Naoto 京都府立医科大学, 医学研究科, 教授 (50347449) ; TAMURA Yutaka 京都府立医科大学, 医学研究科, 講師 (50263174)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 糖脂質代謝 / ﾒﾀﾎﾞﾘｯｸｼﾝﾄﾞﾛｰム / iPS細胞 / 褐色脂肪細胞 / メタボリックシンドローム / ダイレクトコンヴァージョン / ﾒﾀﾎﾞﾘｯｸｼﾝﾄﾞﾛｰﾑ

Research Abstract

In rodents and other animals, brown adipocytes contribute to energy expenditure and heat production, but brown adipocytes of humans have not been characterized in detail, mainly because functional human brown adipocytes that can be propagated in culture have not been available for experimental use so far. We recently established novel procedures to induce human and murine brown adipocytes. In this study we examined gene expression profiles, epigenetic states, and metabolic activities of the brown adipocytes, while we also analyzed regulation of their activities and molecular mechanisms of their induction. The present findings have important implication to novel molecular targeting therapy against metabolic syndromes.

Research Products

• [Journal Article] HIF-1α-induced HSP70 regulates anabolic responses in articular chondrocytes under hypoxic conditions (2014)
  • Author(s): Tsuchida S, Arai Y, Takahashi KA, Kishida T, Terauchi R, Honjo K, Nakagawa S, Inoue H, Ikoma K, Ueshima K, Matsuki T, Mazda O, Kubo T
  • Journal Title: J Orthop Res Volume: (Epub ahead of print) Issue: 8 Pages: 975-980
  • DOI: 10.1002/jor.22623
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/24677016
  • Peer Reviewed / Open Access
• [Journal Article] Sonoporation-mediated transduction of siRNA ameliorated experimental arthritis using 3 MHz pulsed ultrasound (2014)
  • Author(s): Inoue H, Arai Y, Kishida T, Shin-Ya, M, Terauchi R, Nakagawa S, Saito M, Tsuchida, S, Inoue A, Shirai T, Fujiwara H, Mazda O, Kubo T
  • Journal Title: Ultrasonics Volume: 54(3)(Epub 2013 Nov 11) Issue: 3 Pages: 874-81
  • DOI: 10.1016/j.ultras.2013.10.021
  • Peer Reviewed
• [Journal Article] Mild electrical stimulation with heat stimulation increase heat shock protein 70 in articular chondrocyte. (2013)
  • Author(s): Hiraoka N,Arai Y, Takahashi KA, Mazda O, Kishida T, Honjo K, Tsuchida S, Inoue H, Morino S, Suico MA, Kai H, Kubo T.
  • Journal Title: J Orthop Res. Volume: 17 Issue: 6 Pages: 894-900
  • DOI: 10.1002/jor.22307
  • Peer Reviewed
• [Journal Article] Myostatin acts as an autocrine/paracrine negative regulator in myoblast differentiation from human induced pluripotent stem cells. (2013)
  • Author(s): Gao F, Kishida T, Ejima A, Gojo S, Mazda O.
  • Journal Title: Biochem Biophys Res Commun. Volume: 431(2) Issue: 2 Pages: 309-14
  • DOI: 10.1016/j.bbrc.2012.12.105
  • Peer Reviewed
• [Journal Article] Silencing the expression of connexin 43 decreasesinflammation and joint destruction in experimental arthritis. (2013)
  • Author(s): Tsuchida S, Arai Y, Kishida T, Takahashi K, Honjo K, Terauchi R, Inoue H, Oda R, Mazda O, Kubo T.
  • Journal Title: J Orthop Res Volume: 31(4) Issue: 4 Pages: 525-30
  • DOI: 10.1002/jor.22263
  • Peer Reviewed
• [Journal Article] High Pressure Treatment under Subfreezing Temperature Results in Drastic Inactivation of Enveloped and Non-enveloped Viruses (2013)
  • Author(s): Kishida T, Cui FD, Ohgitani E, Gao F, Hayakawa K, Mazda O
  • Journal Title: Biotechnol Lett Volume: 35(8)(Epub 2013 Apr 23) Issue: 8 Pages: 1297-301
  • DOI: 10.1007/s10529-013-1204-8
  • Peer Reviewed
• [Journal Article] High pressure treatment under subfreezing temperature results in drastic inactivation of enveloped and non-enveloped viruses. (2013)
  • Author(s): Kishida T, Cui FD, Ohgitani E, Gao F, Hayakawa K, Mazda O.
  • Journal Title: Biotechnol Lett. Volume: Apr 23 Pages: 1204-8
• [Journal Article] Lansoprazole Inhibits Nitric Oxide and Prostaglandin E(2) Production in Murine Macrophage RAW 264.7 Cells (2012)
  • Author(s): Nakagawa S, Arai Y, Kishida T, Hiraoka N, Tsuchida S, Inoue H, Sakai R, Mazda O, Kubo T
  • Journal Title: Inflammation Volume: 35(3)(PMID :22134422)(PubMed - in process) Pages: 1062-8
  • Peer Reviewed
• [Journal Article] Lansoprazole Inhibits Nitric Oxide and Prostaglandin E2 Production in Murine Macrophage RAW 264.7 Cells (2012)
  • Author(s): Shuji Nakagawa, Yuji Arai, Tsunao Kishida, Nobuyuki Hiraoka, Shinji Tsuchida, Hiroaki Inoue, Ryo Sakai, Osam Mazda, Toshikazu Kubo
  • Journal Title: Inflammation Volume: Vol.35 Issue: 3 Pages: 1062-1068
  • DOI: 10.1007/s10753-011-9412-7
  • Peer Reviewed
• [Journal Article] IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or IgE Production (2011)
  • Author(s): Tamagawa-Mineoka R, Kishida T, Mazda O, Katoh N
  • Journal Title: J Invest Dermatol Volume: 131(7)(Epub 2011 Mar24) Issue: 7 Pages: 1513-20
  • DOI: 10.1038/jid.2011.73
  • Peer Reviewed
• [Journal Article] Interleukin-28B acts synergistically with cisplatin to suppress the growth of head and neck squamous cell carcinoma. (2011)
  • Author(s): Yoshimoto K, Kishida T, Nakano H, Matsui M, Shin-Ya M, Shimada T, Nakai S, Imanishi J, Takeuchi M, Hisa Y, Mazda O.
  • Journal Title: J Immunother. Volume: 34(2） Issue: 2 Pages: 139-48
  • DOI: 10.1097/cji.0b013e318204ed70
  • Peer Reviewed
• [Journal Article] Intra-articular injection of hyaluronan restores the aberrant expression of matrix metalloproteinase-13 in osteoarthritic subchondral bone. (2011)
  • Author(s): Hiraoka N, Takahashi KA, Arai Y, Sakao K, Mazda O, Kishida T, Honjo K, Tanaka S, Kubo T
  • Journal Title: J Orthop Res. Volume: 29(3) Issue: 3 Pages: 354-60
  • DOI: 10.1002/jor.21240
  • Peer Reviewed
• [Journal Article] Pleiotropic functions of magnetic nanoparticles for ex vivo gene transfer
  • Author(s): Kami D, Kitani T, Kishida T, Mazda O, Toyoda M, Tomitaka A, Ota S, Ishii R, Takemura Y, Watanabe M, Umezawa A, Gojo S
  • Journal Title: Nanomedicine : Nanotechnology, Biology and Medicine Volume: (in press)
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/24709330
  • Peer Reviewed
• [Presentation] Anti-inflammatory effects of lansoprazole by suppressing ROS production in murine macrophage RAW 264.7 cells (2014)
  • Author(s): S. Ichimaru, S. Nakagawa, Y. Arai, S. Tsuchida, H. Inoue, T. Matsuki, Y. Mikami, T. Ikeda, R. Oda, O. Mazda, T. Kubo
  • Organizer: 2014 Orthopaedic Research Society Annual Meeting
  • Place of Presentation: New Orleans, Louisiana, USA
  • Year and Date: 2014-03-16
• [Presentation] Sonoporation-mediated transduction of siRNA targeting TNF alpha ameliorated experimental arthritis (2014)
  • Author(s): Inoue H, Arai Y, Nakagawa S, Saito M ,Tsuchida S, Inoue A, Matsuki T, Morihara T, Shirai T, Mazda, O, Kubo T
  • Organizer: 60th Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: New Orleans, Louisiana, USA
• [Presentation] Femoral perfusion after electromagnetic fields stimulation to the steroid-induced osteonecrosis model (2014)
  • Author(s): A. Ikegami, K. Ueshima, K. Ikoma, M. Fujioka, M. Saito, S. Hayashi, M. Ishida, M. Kuribayashi, O. Mazda and T. Kubo
  • Organizer: ORS 60th Annual Meeting
  • Place of Presentation: New Orleans, LA
• [Presentation] Evaluation of femoral perfusion in a rabbit model of steroid-induced osteonecrosis with a high magnetic field MRI system (2014)
  • Author(s): S. Hayashi, M. Fujioka, K. Ikoma, M. Saito, K. Ueshima, M. Ishida, M. Kuribayashi, A. Ikegami, O. Mazda and T. Kubo
  • Organizer: 60th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: New Orleans, Louisiana, USA
• [Presentation] Effect of mechanical stress on the hypertrophic differentiation related gene expression in cultured chondrocytes (2013)
  • Author(s): Inoue H, Arai Y, Terauchi R, Nakagawa S, Saito M, Hiraoka N, Tsuchida S, Matsuki T, Mazda Osam, Kubo T
  • Organizer: 16th World Congress of the Osteoarthritis Research Society International
  • Place of Presentation: Philadelphia, USA
• [Presentation] The roles of heat shock protein 70 on chondrocyte (2013)
  • Author(s): Matsuki T, Arai Y, Tsuchida S, Terauchi R, Inoue H, Nakagawa S, Inoue A, Mazda O, Kubo T
  • Organizer: 16th World Congress of the Osteoarthritis Research Society International
  • Place of Presentation: Philadelphia, USA
• [Patent(Industrial Property Rights)] 褐色脂肪細胞及びその調製方法. (2012)
  • Inventor(s): 岸田綱郎、松田 修.
  • Industrial Property Rights Holder: 岸田綱郎、松田 修.
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-07-12
• [Patent(Industrial Property Rights)] 骨芽細胞の調製方法 (2012)
  • Inventor(s): 山本健太、岸田綱郎、山本俊郎、松田修
  • Industrial Property Rights Holder: 山本健太、岸田綱郎、山本俊郎、松田修
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-07-02