| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Ghrelin reportedly restricts insulin release in islet b-cells via Gi-proteins and thereby regulates glucose homeostasis. This study explored whether ghrelin regulates cAMP signaling and whether this regulation induces insulinostatic cascade in islet b-cells. Furthermore, this study aimed to clarify whether ghrelin counteracts effects of GLP-1, a physiological incretin hormone that promote glucose-induced insulin release in rat islet b-cells.
Ghrelin directly interacts with b-cells to attenuate glucose-induced cAMP production and PKA activation, which lead to activation of Kv channels and suppression of glucose-induced [Ca2+]i increase and insulin release. Ghrelin potently attenuates GLP-1-induced cAMP production, [Ca2+]i increases and insulin release in b-cells. These findings indicate that interaction between ghrelin and GLP-1 plays an important role in physiological regulation of glucose-induced insulin release.
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