Role of AMPK in HDL-induced antiarterogenic effects in endothelial cells

• Project/Area Number: 23591331
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Gunma University
• Principal Investigator: KIMURA Takao 群馬大学, 医学(系)研究科(研究院), 講師 (90396656)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 脂質代謝異常 / 動脈硬化 / HDL / 血管内皮細胞 / S1P / スカベンジャー受容体 / スフィンゴシン１－リン酸 / AMPK

Research Abstract

High density lipoprotein (HDL) stimulated AMP kinase (AMPK) in human umbilical vein endothelial cells (HUVECs). HDL activated AMPK through independent two pathways. HDL-induced AMPK activation was mediated by sphingosine-1 phosphate (S1P)/S1P receptor pathway and scavenger receptor (SR-BI)/PDZK-1. S1P/S1P receptor pathway was mediated by calmodulin. SR-BI/PDZK-1 pathway was mediated by calmodulin and LKB1 in HUVECs. Simvastatin, fenofibrate, metrformin and AICAR enhanced protein expression of SR-BI and endothelial nitric oxide synthase (eNOS) in HUVECs. Simultaneous pretreatment of simvastatin and fenofibrate enhanced HDL-induced AMPK/Akt activation in HUVECs.

Research Products

• [Journal Article] Seroprevalence of Measles- and Mumps-Specific Immunoglobulin G among Japanese Healthcare Students Increased during 2007-2012 (2013)
  • Author(s): Kimur a T, Tsunekawa K, Ogiwara T, Tokue Y, Nara M, Inoue T, Obuchi T, Suto C, Ohshima K, Murakami M
  • Journal Title: Jpn J Infect Dis Volume: 66(5) Pages: 411-415
  • URL: https://www.jstage.jst.go.jp/article/yoken/66/5/66_411/_article
  • Peer Reviewed
• [Journal Article] Tuberculosis screening using a T-cell interferon-γrelease assay in Japanese medical students and non-Japanese international students (2013)
  • Author(s): Ogiwara T, Kimur a T, Tokue Y, Watanabe R, Nara M, Obuchi T, Yaegashi A, Yomoda S, Ohshima K, Murakami M
  • Journal Title: Tohoku J Exp Med Volume: 230(2) Pages: 87-91
  • URL: https://www.jstage.jst.go.jp/article/tjem/230/2/230_87/_article
  • Peer Reviewed
• [Journal Article] Seroprevalence of Measles- and Mumps-Specific Immunoglobulin G among Japanese Healthcare Students Increased during 2007-2012. (2013)
  • Author(s): 1. Kimura T, Tsunekawa K, Ogiwara T, Tokue Y, Nara M, Inoue T, Obuchi T, Suto C, Ohshima K, Murakami M.
  • Journal Title: Jpn J Infect Dis. Volume: 66 Pages: 411-415
  • NAID: 130003381718
  • Peer Reviewed
• [Journal Article] Tuberculosis screening using a T-cell interferon-γ release assay in Japanese medical students and non-Japanese international students. (2013)
  • Author(s): 15. Ogiwara T, Kimura T, Tokue Y, Watanabe R, Nara M, Obuchi T, Yaegashi A, Yomoda S, Ohshima K, Murakami M.
  • Journal Title: Tohoku J Exp Med. Volume: 230 Pages: 87-91
  • NAID: 10031182996
  • Peer Reviewed
• [Journal Article] Doppler echocardiography may provide a potentially life-saving screening of anomalous origin of coronary artery in young athletes (2012)
  • Author(s): Nakahara T, Takahashi-Tateno R, Hasegawa A, Kimur a T, Tsushima Y, Murakami M, Kurabayashi M
  • Journal Title: Int J Cardiol Volume: 5;156(1) Pages: 104-105
  • URL: http://www.sciencedirect.com/science/article/pii/S0167527312000071
  • Peer Reviewed
• [Journal Article] An orally active lysophosphatidic acid receptor antagonist attenuates panereatic cancer invasion and metastasis in vivo (2012)
  • Author(s): Komachi M
  • Journal Title: Cancer Science Volume: (印刷中) Issue: 6 Pages: 1099-1104
  • DOI: 10.1111/j.1349-7006.2012.02246.x
  • Peer Reviewed
• [Presentation] 血清中アポリポ蛋白M測定の臨床的意義の検討 (2013)
  • Author(s): 木村孝穂、角野博之、岡島史和、村上正巳
  • Organizer: 第60回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2013-11-02
• [Presentation] 血清中アポリポ蛋白M測定の臨床的意義 (2013)
  • Author(s): 木村孝穂、角野博之、岡島史和、村上正巳
  • Organizer: 第45回日本動脈硬化学会総会・学術集会シンポジウム
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2013-07-19
• [Presentation] アポリポ蛋白Ｍ測定の臨床的意義 (2013)
  • Author(s): 木村孝穂、角野博之、岡島史和、村上正巳
  • Organizer: 第45回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 東京都新宿区京王プラザホテル
  • Invited
• [Presentation] 血清中アポリポ蛋白Ｍ測定の 臨床的意義の検討 (2013)
  • Author(s): 木村孝穂、角野博之、荒木修、常川勝彦、 青木智之、荻原貴之、奈良誠人、町田哲男、 桑原敦志、岡島史和、村上正巳
  • Organizer: 第60回臨床検査医学会総会・学術集会
  • Place of Presentation: 兵庫県神戸市神戸国際会議場
• [Presentation] Tuberculosis screening by a T cell interferon-release assay in students of medical school and international students in Gunma University (2012)
  • Author(s): R. Watanabe, T. Kimur a, Y. Tokue, T. Ogiwara, M. Nara, Y. Kobayashi, T. Inoue, H. Sumino, T. Morimura, O. Araki, K. Tsunekawa, T. Aoki, T. Obuchi, Y. Yomoda, K. Ohshima, M. Murakami
  • Organizer: The12^<th> meeting of the Asian Society of Clinical Pathology and Laboratory Medicine
  • Place of Presentation: 京都国際会館
  • Year and Date: 2012-12-01
• [Presentation] 正常ヒト臍帯静脈内皮細胞(HUVEC) における甲状腺ホルモンのnon-genomic effectの検討 (2012)
  • Author(s): 青木 智之、常川 勝彦、森村 匡志、荒木 修、奈良 誠人、荻原貴之、角野博之、木村孝穂ほか
  • Organizer: 第５５回日本甲状腺学会学術集会
  • Place of Presentation: アクロス福岡
• [Presentation] Induction of SR-BI and eNOS expression by cilostazol enhanced HDL-induced anti-atherogenic actions in endothelial cells (2011)
  • Author(s): T. Kimur a, M. Murakami and F. Okajima
  • Organizer: 第43回日本動脈硬化学会総会・学術集会
  • Place of Presentation: ロイトン札幌ホテル
  • Year and Date: 2011-07-15
• [Presentation] 血管内皮細胞における甲状腺ホルモンのeNOS活性化作用と脱ヨード酵素の役割
  • Author(s): 木村孝穂、青木智之、村上正巳ほか
  • Organizer: 第５４回日本甲状腺学会学術集会
  • Place of Presentation: 大阪国際交流センター（大阪）