Pathophysiology of secretary form of VLDL receptor

• Project/Area Number: 23591335
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: University of Fukui
• Principal Investigator: TAKAHASHI Sadao 福井大学, 医学部, 特別研究員 (50303376)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: VLDL受容体 / 選択的スプライシング / ヒト血中分泌型VLDL受容体 / 臨床意義 / 生理機能

Research Abstract

In vivo and in vitro studies have shown that the VLDL receptor bind TG-rich lipoprotein (TGRL) but not LDL and function as a peripheral lipoprotein receptor even though the LDL receptor bind both TGRL and LDL. Two VLDL receptor proteins (type1 and type2) are produced by alternative splicing mechanism. We elucidated that type2 VLDL receptor lacking the O-linked sugar domain bound lower affinity for TGRL than type1 VLDL receptor. Type1 VLDL receptor was expressed predominantly during monocyte-macrophage differentiation and growth. Type2 VLDL receptor was secreted into medium and we established the method for measurement of human secertary form of VLDL receptor (sVLDL-R). Level of human sVLDL-R in diabetes was higher than healthy control.

Research Products

• [Journal Article] Differential effects ofα-glucosidase inhibitors on postprandial plasma glucose and lipid profile in patients with type 2 diabetes under control with insulin Lispro mix 50/50 (2012)
  • Author(s): Kimura T, Suzuki J, Ichikawa M, Imagawa M, Sato S, Fujii M, Zenimaru Y, Inaba S, Takahashi S, Konoshita T, Miyamori I
  • Journal Title: Diabetes Technol. Ther. Volume: 14 Pages: 545-551
  • Peer Reviewed
• [Journal Article] Comparative reactivity of remnant-like lipoprotein particles (RLP) and low-density lipoprotein (LDL) to LDL receptor and VLDL receptor : Effect of a high-dose statin on VLDL receptor expression (2012)
  • Author(s): Imagawa M, Takahashi S, Zenimaru Y, Kimura T, Suzuki J, Miyamori I, Iwasaki T, Hattori H, Yamamoto TT, Nakano T, Nakajima K
  • Journal Title: Clin Chim Acta Volume: 413 Pages: 441-447
  • Peer Reviewed
• [Journal Article] Differential effects of α-glucosidase inhibitors on postprandial plasma glucose and lipid profile in patients with type 2 diabetes under control with insulin lispro mix 50/50. (2012)
  • Author(s): Kimura T, Konoshita T et al.
  • Journal Title: Diabetes Technol Ther Volume: 14 Issue: 7 Pages: 545-551
  • DOI: 10.1089/dia.2012.0015
  • Peer Reviewed
• [Journal Article] Comparative reactivity of remnant-like lipoprotein particles (RLP) and low-density lipoprotein (LDL) to LDL receptor and VLDL receptor: effect of a high-dose statin on VLDL receptor expression (2012)
  • Author(s): Imagawa M
  • Journal Title: Clin Chim Acta Volume: 413 Issue: 3-4 Pages: 441-447
  • DOI: 10.1016/j.cca.2011.10.033
  • Peer Reviewed
• [Journal Article] Species differences of macrophage very low-density-lipoprotein (VLDL) receptor protein expression (2011)
  • Author(s): Takahashi S, Ito T, Zenimaru Y, Suzuki J, Miyamori I, Takahashi M, Takahashi M, Ishida T, Ishida T, Hirata K, Yamamoto TT, Iwasaki T, Hattori H, Shiomi M
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 407 Pages: 656-662
  • Peer Reviewed
• [Presentation] 分泌型VLDL受容体の存在とヒト血中濃度測定法の確立 (2013)
  • Author(s): 高橋貞夫,銭丸康夫鈴木仁弥今川美智子佐藤さつき山本勝司,山田実夏市川麻衣石塚全,服部浩明
  • Organizer: 第17回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
• [Presentation] 分泌型VLDL受容体の存在とヒト血中濃度測定法の確立 (2013)
  • Author(s): 高橋貞夫，銭丸康夫 鈴木仁弥 今川美智子 佐藤さつき 山本勝司，山田実夏 市川麻衣 石塚 全，服部浩明．
  • Organizer: 第17回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 千里ライフサイエンスセンター
• [Presentation] Presence of human soluble very-low-density lipoprotein (VLDL) receptor in serum (2012)
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Ichikaewa M, Sato S, Suzuki J, Miyamori I, Iwasaki T, Hattori H
  • Organizer: XVI International Symposium on Atherosclerosis
  • Place of Presentation: Sydney Convention and Exhibition Centre, Australia
• [Presentation] Central role of lipoprotein lipase and very low-density lipoprotein receptor on macrophage foam cell formation by triglyceride-rich lipoproteins and low density lipoprotein (2011)
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Suzuki J, Miyamori I
  • Organizer: 47^<th> EASD Annual Meeting
  • Place of Presentation: Lisbon
• [Presentation] Species differences of macrophage very low-density lipoprotein (VLDL) receptor protein expression (2011)
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Suzuki J, Miyamori I, Yamamoto TT, Ito T, Shiomi M
  • Organizer: AHA, ATVB meeting 2011
  • Place of Presentation: Chicago Hilton Hotel, Chicago
• [Presentation] Species Differences of Macrophage Very Low-Density Lipoprotein (VLDL) Receptor Protein Expression. (2011)
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Suzuki J, Miyamori I, Yamamoto TT, Ito T, Shiomi M.
  • Organizer: AHA, ATVB meeting 2011
  • Place of Presentation: Chicago
• [Presentation] Central role of lipoprotein lipase and very low-density lipoprotein receptor on macrophage foam cell formation by triglyceride-rich lipoproteins and low density lipoprotein (2011)
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Suzuki J, Miyamori I.
  • Organizer: 47th EASD Annual Meeting
  • Place of Presentation: Lisbon
• [Presentation] TGリッチリポ蛋白と動脈硬化巣形成機序 (2011)
  • Author(s): 高橋貞夫
  • Organizer: 第26回日本糖尿病合併症学会
  • Place of Presentation: 大宮
• [Presentation] Presence of human soluble very-low-density lipoprotein (VLDL) receptor in serum
  • Author(s): Takahashi S, Zenimaru Y, Imagawa M, Kimura T, Ichikaewa M, Sato S, Suzuki J, Miyamori I, Iwasaki T, Hattori H.
  • Organizer: XVI International Symposium on Atherosclerosis (ISA 2012)
  • Place of Presentation: Sydney Convention and Exhibition Centre (Australia)
• [Presentation] VLDL受容体isoformとヒト血清分泌型VLDL受容体測定法の確立
  • Author(s): 銭丸康夫，今川美智子，市川麻衣，佐藤さつき，木村朋子，稲葉 聡， 鈴木仁弥，高橋貞夫，岩崎忠雄，服部浩明
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: パシフィコ横浜（横浜）
• [Book] VLDLR:疾患モデルの作製と利用—脂質代謝異常と関連疾患 (2014)
  • Author(s): 高橋貞夫
  • Publisher: エル・アイ・シー出版(印刷中)
• [Book] 疾患モデルの作製と利用―脂質代謝異常と関連疾患 (2014)
  • Author(s): 高橋貞夫
  • Total Pages: 10
  • Publisher: エル・アイ・シー出版