Studies of TICE and intesitinal SR-BI
| Project/Area Number |
23591338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Nagoya City University |
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Principal Investigator |
TSUJITA Maki 名古屋市立大学, 医学(系)研究科(研究院), 講師 (10253262)
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOYAMA Shinji 中部大学, 次世代食育センター, 教授 (10142192)
UGAWA Shinya 名古屋市立大学, 大学院医学研究科, 教授 (20326135)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | マウス / 小腸 / HDL / TICE / SR-BI / cholesterol / urea |
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| Outline of Final Research Achievements |
Transintestiinal cholesterol efflux (TICE) is the alternative cholesterol secretion pathway which has been focus for some years. To evaluate the fate of HDL-cholesterol in mice plasma, we injected 3H-labeled cholesterylether in HDL. SR-BI is known for HDL-cholesterylester receptor. In SR-BI null mice, the uptake of chosteryleter from injected HDL was significantly reduced liver and adrenalas well as in small intestine. Next, wild type mouse was perfused with PBS followed by the fixation solution (4%PFA/0.1M PB).SR-BI was detected by primary antibody and streptavidin bound Alexa Fluor594. The Nicon A1RS1 confocal microscope system was used for detection. SR-BI was stained at basolateral surface of some small intestinal absorptive epithelial cells. As control, intestinal cholesterol receptor, NPC1L1 was localized only in the apical surface in this experimental condition. This result suggests plasma HDL-cholesterol may uptake into small intestinal cells through SR-BI in mice.
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Report
(5 results)
Research Products
(12 results)
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[Presentation] 血漿HDL－コレステロールの小腸への搬出はSR-BIを介する2014
Author(s)
Maki Tsujita, Tomo Yokota, Frank J Gonzalez, Shinya Ugawa, Shinji Yokoyama
Organizer
第87回日本生化学会
Place of Presentation
国立京都国際会館 (京都府・京都市）
Year and Date
2014-10-15 – 2014-10-18
Related Report
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[Presentation] ABCG1 null and SR-BI null mice reduced plasma HDL uptake by liver and small intestine.2014
Author(s)
Maki Tsujita, Nobukatsu Akita, M Anwar Hossain, Tomo Yokota, Alan T Remaley, Natsuko Kumamoto, Takashi Ueda, Shinya Ugawa, Shinji Yokoyama
Organizer
ATVB 2014 Scientific Sessions
Place of Presentation
Sheraton Centre Toronto Hotel, Toronto, Ontario, Canada
Related Report
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[Presentation] An anion-exchange chromatography isolated sub-fraction of mouse apolipoprotein A-I is unable to activate cellular cholesterol release from mouse peritoneal macrophage foam cells.2012
Author(s)
Okumura-Noji Kuniko, Cavigiolio Giorgio, Huang Rong, Davidson W Sean, Akita Nobukatsu, Okuhira Kei-ichiro, Yokoyama Shinji, Tsujita Maki.
Organizer
Arteriosclerosis, Thrombosis and Vascular Biology 2012 Scientific Sessions
Place of Presentation
Hilton Chicago, Chicago, Il, USA.
Related Report
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[Presentation] Heterogeneity of human and mouse apolipoprotein A-I fractioned revealed by ion-exchange chromatography.2012
Author(s)
Okumura-Noji K, Huang R, Akita N, Remaley AT, Gonzalez FJ, Yokoyama S, Davidson WS , Tsujita M.
Organizer
International Symposium on Atherosclerosis Satellite Symposium High Density Lipoproteins: biology and therapeutics
Place of Presentation
Hilton Hotel Cairns, Cairns, TNQ, Australia
Related Report
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