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Studies of TICE and intesitinal SR-BI

Research Project

Project/Area Number 23591338
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metabolomics
Research InstitutionNagoya City University

Principal Investigator

TSUJITA Maki  名古屋市立大学, 医学(系)研究科(研究院), 講師 (10253262)

Co-Investigator(Renkei-kenkyūsha) YOKOYAMA Shinji  中部大学, 次世代食育センター, 教授 (10142192)
UGAWA Shinya  名古屋市立大学, 大学院医学研究科, 教授 (20326135)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsマウス / 小腸 / HDL / TICE / SR-BI / cholesterol / urea
Outline of Final Research Achievements

Transintestiinal cholesterol efflux (TICE) is the alternative cholesterol secretion pathway which has been focus for some years. To evaluate the fate of HDL-cholesterol in mice plasma, we injected 3H-labeled cholesterylether in HDL. SR-BI is known for HDL-cholesterylester receptor. In SR-BI null mice, the uptake of chosteryleter from injected HDL was significantly reduced liver and adrenalas well as in small intestine. Next, wild type mouse was perfused with PBS followed by the fixation solution (4%PFA/0.1M PB).SR-BI was detected by primary antibody and streptavidin bound Alexa Fluor594. The Nicon A1RS1 confocal microscope system was used for detection. SR-BI was stained at basolateral surface of some small intestinal absorptive epithelial cells. As control, intestinal cholesterol receptor, NPC1L1 was localized only in the apical surface in this experimental condition. This result suggests plasma HDL-cholesterol may uptake into small intestinal cells through SR-BI in mice.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (12 results)

All 2014 2012 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (11 results) (of which Invited: 1 results)

  • [Journal Article] Calpain-mediated ABCA1 degradation : Post-translational regulation of ABCA1 for HDL biogenesis2011

    • Author(s)
      Yokoyama S, Arakawa R, Wu CA, Iwamoto N, Lu R, Tsujita M, Abe-Dohmae S
    • Journal Title

      Biochim Biophys Acta 2012 Mar

      Volume: 1821(3) Issue: 3 Pages: 547-551

    • DOI

      10.1016/j.bbalip.2011.07.017

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 血漿HDL-コレステロールの小腸への搬出はSR-BIを介する2014

    • Author(s)
      Maki Tsujita, Tomo Yokota, Frank J Gonzalez, Shinya Ugawa, Shinji Yokoyama
    • Organizer
      第87回日本生化学会
    • Place of Presentation
      国立京都国際会館 (京都府・京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高脂血症治療剤ProbucolのHDL新生反応阻害と低HDL血症モデル動物繁殖への有効性2014

    • Author(s)
      辻田麻紀
    • Organizer
      第15回 Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      名古屋市立大学大学院薬学研究科・宮田専治記念ホール (愛知県・名古屋市)
    • Year and Date
      2014-05-23 – 2014-05-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ABCG1 null and SR-BI null mice reduced plasma HDL uptake by liver and small intestine.2014

    • Author(s)
      Maki Tsujita, Nobukatsu Akita, M Anwar Hossain, Tomo Yokota, Alan T Remaley, Natsuko Kumamoto, Takashi Ueda, Shinya Ugawa, Shinji Yokoyama.
    • Organizer
      Arteriosclerosis, Thrombosis and Vascular Biology 2014 Scientific Sessions
    • Place of Presentation
      Toronto (Canada)
    • Year and Date
      2014-05-01 – 2014-05-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] ABCG1 null and SR-BI null mice reduced plasma HDL uptake by liver and small intestine.2014

    • Author(s)
      Maki Tsujita, Nobukatsu Akita, M Anwar Hossain, Tomo Yokota, Alan T Remaley, Natsuko Kumamoto, Takashi Ueda, Shinya Ugawa, Shinji Yokoyama
    • Organizer
      ATVB 2014 Scientific Sessions
    • Place of Presentation
      Sheraton Centre Toronto Hotel, Toronto, Ontario, Canada
    • Related Report
      2013 Research-status Report
  • [Presentation] Stress-mediated acute corticosterone production is depending on plasma HDL level but not long-term stress response in mice.2012

    • Author(s)
      Maki Tsujita, Nobukatsu Akita, M Anwar Hossain, Frank J Gonzalez, Shinji Yokoyama, and Makoto Michikawa
    • Organizer
      The 50th Anniversary Symposium on Cytochrome P450 in Fukuoka
    • Place of Presentation
      Kyushu University Center-I for Collaborative Research, Fukuoka, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] An anion-exchange chromatography isolated sub-fraction of mouse apolipoprotein A-I is unable to activate cellular cholesterol release from mouse peritoneal macrophage foam cells.2012

    • Author(s)
      Okumura-Noji Kuniko, Cavigiolio Giorgio, Huang Rong, Davidson W Sean, Akita Nobukatsu, Okuhira Kei-ichiro, Yokoyama Shinji, Tsujita Maki.
    • Organizer
      Arteriosclerosis, Thrombosis and Vascular Biology 2012 Scientific Sessions
    • Place of Presentation
      Hilton Chicago, Chicago, Il, USA.
    • Related Report
      2012 Research-status Report
  • [Presentation] Heterogeneity of human and mouse apolipoprotein A-I fractioned revealed by ion-exchange chromatography.2012

    • Author(s)
      Okumura-Noji K, Huang R, Akita N, Remaley AT, Gonzalez FJ, Yokoyama S, Davidson WS , Tsujita M.
    • Organizer
      International Symposium on Atherosclerosis Satellite Symposium High Density Lipoproteins: biology and therapeutics
    • Place of Presentation
      Hilton Hotel Cairns, Cairns, TNQ, Australia
    • Related Report
      2012 Research-status Report
  • [Presentation] 高グルコースにより誘発される低HDL産生の分子制御機構の解明High D-glucose reduced apoA-I and HDL generation in liver cells.2012

    • Author(s)
      辻田麻紀、秋田展克、横山信治、野路久仁子、道川 誠
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] Plasma prebeta-HDL in hypo-, normal- and hyper-alphalipoproteinemia model mice2012

    • Author(s)
      Tomo Yokota, Kuniko Okumura-Noji, Nobukatsu Akita, Giorgio Cavigiolio, Alan T Remaley, Maki Tsujita, Shinji Yokoyama
    • Organizer
      4th FEBS Special Meeting, ATP-binding Cassette(ABC) Proteins: From Multidrug Resistance to Genetic Diseases
    • Place of Presentation
      Innsbruck, Austria
    • Related Report
      2011 Research-status Report
  • [Presentation] Characterization of ion-exchange column fractionated human and mouse apoA-I subfractions.2011

    • Author(s)
      Kuniko Okumura-Noji, Toshihiko Usami, Giorgio Cavigiolio, Rong Huang, W Sean Davidson, Shinji Yokoyama, Maki Tsujita.
    • Organizer
      Arteriosclerosis, Thrombosis and Vascular Biology 2011 Scientific Sessions
    • Place of Presentation
      Chicago, Il, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] 尿素ポリアクリルアミド電気泳動法を用いたヒト並びにマウス血漿apoA-Iの解析/ Analyses of human and mouse apo A-I in 6M Urea PAGE2011

    • Author(s)
      辻田麻紀、宇佐美寿彦、秋田展克、Frank J Gonzalez、横山信治、野路久仁子
    • Organizer
      第84回日本生化学会
    • Place of Presentation
      国立京都国際会館、京都
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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