| Project/Area Number |
23591356
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIHARA Naotada 久留米大学, 分子生命科学, 教授 (10325516)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ミトコンドリアダイナミクス / インスリン分泌 / Drp1 / 小胞体ストレス / 脂肪肝炎 / 糖尿病 / 非アルコール性脂肪肝炎 / インスリン抵抗性 / 臓器間ネットワーク |
|---|
| Research Abstract |
Mitochondria have essential roles in the aerobic generation of ATP by oxidative phosphorylation, as well as in variety of cellular processes such as apoptosis and Ca2+ signaling. The mitochondrial morphology changes dynamically by a balanced fusion and fission processes that occur in response to cellular energy demands, differentiation or pathogenic conditions. In order to clarify the roles of mitochondrial fission in pancreatic beta cells and hepatocytes in vivo, we used a Cre-loxP system to disrupt Drp1 in pancreatic beta cells (Drp1betaKO) and hepatocytes (Drp1LiKO), respectively. Drp1betaKO mice showed defective insulin secretion in response to glucose and overt diabetes. Upon high fat diet, Drp1LiKO mice developed non-alcoholic steatohepatitis with marked elevation of the endoplasmic reticulum stress markers.
Collectively, mitochondrial dynamics plays important roles in pancreas beta cell and hepatocytes to meet energy demand.
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