Investigation for the role of UBE2E2 in insulin secretory machinery and its molecular mechanisms

• Project/Area Number: 23591361
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Endocrinology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MINAKO Imamura 独立行政法人理化学研究所, 統合生命医科学研究センター, 研究員 (00596124)
• Co-Investigator(Kenkyū-buntansha): MAEDA Shiro 独立行政法人理化学研究所, 統合生命医科学研究センター, チームリーダー (50314159)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 2型糖尿病 / 遺伝要因 / 遺伝子

Research Abstract

We have evaluated the insulin secretion from pancreatic beta-cell line and phenotype of the genetically- modified mice for Ube2e2 to explore the role of UBE2E2 in pancreatic beta-cells. Our results indicate that it is important to consider the influence of environmental factor(s), such as age and obesity, to reveal the mechanism(s) how UBE2E2 contribute to conferring susceptibility to type 2 diabetes. We have also identified several candidates for causal variants by a fine- mapping within the UBE2E2 locus.

Research Products

• [Journal Article] Genome-wide association study identifies three novel loci for type 2 diabetes (2014)
  • Author(s): Hara K, Fujita H, Johnson TA, Yamauchi T, Yasuda K, Horikoshi M, Peng C, Hu C, Ma RC, Imamura M, et al
  • Journal Title: Hum Mol Genet Volume: 23 Issue: 1 Pages: 239-246
  • DOI: 10.1093/hmg/ddt399
  • Peer Reviewed / Open Access
• [Journal Article] 特集 糖尿病と遺伝子 2型糖尿病における進歩;ゲノムワイド関連解析による疾患感受性遺伝子領域同定の成果 (2014)
  • Author(s): 今村美菜子 前田士郎
  • Journal Title: 糖尿病 Volume: 57 Pages: 85-87
• [Journal Article] Assessing the clinical utility of a genetic risk score constructed using 49 susceptibility alleles for type 2 diabetes in a Japanese population (2013)
  • Author(s): Imamura M, Shigemizu D, Tsunoda T, Iwata M, Maegawa H, Watada H, Hirose H, Tanaka Y, Tobe K, Kaku K, Kashiwagi A, Kawamori R, Maeda S
  • Journal Title: J Clin Endocrinol Metab Volume: 98(10) Issue: 10 Pages: E1667-E1673
  • DOI: 10.1210/jc.2013-1642
  • Peer Reviewed
• [Journal Article] Replication study for the association of rs391300 in SRR and rs17584499 in PTPRD with susceptibility to type 2 diabetes in a Japanese population (2013)
  • Author(s): Imamura M, Iwata M, Maegawa H, Watada H, Hirose H, Tanaka Y, Tobe K, Kaku K, Kashiwagi A, Kadowaki T, Kawamori R and Maeda S
  • Journal Title: J Diabetes Invest Volume: Volume 4 Issue 2 Issue: 2 Pages: 168-173
  • DOI: 10.1111/jdi.12017
  • Peer Reviewed
• [Journal Article] バイオバンクジャパンの成果と今後;2型糖尿病疾患感受性遺伝子研究の進歩 (2013)
  • Author(s): 今村美菜子、久保充明、前田士郎
  • Journal Title: Diabetes Frontier Volume: vol 24 no4 Pages: 377-384
• [Journal Article] Replication study for the association of 9 East Asian GWAS-derived loci with susceptibility to type 2 diabetes in a Japanese population (2013)
  • Author(s): Sakai K, Imamura M, Tanaka Y, Iwata M, Hirose H, Kaku K, Maegawa H, Watada H, Tobe K, Kashiwagi A, Kawamori R, Maeda S
  • Journal Title: PLoS One Volume: 8 Issue: 9 Pages: e76317-e76317
  • DOI: 10.1371/journal.pone.0076317
  • Peer Reviewed
• [Journal Article] Replication study for the association of a single-nucleotide polymorphism, rs3746876, within KCNJ15, with susceptibility to type 2 diabetes in a Japanese population (2013)
  • Author(s): Fukuda H, Imamura M, Tanaka Y, Iwata M, Hirose H, Kaku K, Maegawa H, Watada H, Tobe K, Kashiwagi A, Kawamori R, Maeda S.
  • Journal Title: J Hum Genet Volume: 58 Issue: 7 Pages: 490-493
  • DOI: 10.1038/jhg.2013.28
  • NAID: 10031190046
  • Peer Reviewed
• [Journal Article] 糖代謝関連形質にかかわる遺伝子領域 (2013)
  • Author(s): 今村 美菜子
  • Journal Title: 医学のあゆみ Volume: 244 Pages: 1025-1029
• [Journal Article] バイオバンクジャパンの成果と今後；2型糖尿病疾患感受性遺伝子研究の進歩 (2013)
  • Author(s): 今村美菜子、久保充明、前田士郎
  • Journal Title: Diabetes Frontier Volume: 24 Pages: 377-384
• [Journal Article] Replication study for the association of rs391300 in SRR and rs17584499 in PTPRD with susceptibility to type 2 diabetes in a Japanese population (2013)
  • Author(s): Imamura M, Iwata M, Maegawa H, Watada H, Hirose H, Tanaka Y, Tobe K, Kaku K, Kashiwagi A, Kadowaki T, Kawamori R and Maeda S
  • Journal Title: J Diabetes Invest Volume: 4 Pages: 168-173
  • Peer Reviewed
• [Journal Article] The influence of a single nucleotide polymorphism within CNDP1 on susceptibility to diabetic nephropathy in Japanese women in type 2 diabetes (2013)
  • Author(s): Kurashige M, Imamura M, Araki S, Suzuki D, Babazono T, Suzuki T, Umezono T, Toyoda M, Kawai K, Imanishi M, Hanaoka K, Maegawa H, Uchigata Y, Hosoya T, Maeda S
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e54064-e54064
  • DOI: 10.1371/journal.pone.0054064
  • Peer Reviewed
• [Journal Article] A single-nucleotide polymorphism in ANK1 is associated with susceptibility to type 2 diabetes in Japanese populations. (2012)
  • Author(s): Imamura M, Maeda S, Yamauchi T, Hara K, Yasuda K,..., Okada-Iwabu M, Iwabu M, Shojima N,..., Nakamura Y, Kadowaki T
  • Journal Title: Human Molecular Genetics Volume: 21 Issue: 13 Pages: 3042-3049
  • DOI: 10.1093/hmg/dds113
  • Peer Reviewed
• [Journal Article] 糖尿病疾患感受性遺伝子の解明 (2012)
  • Author(s): 今村美菜子、前田士郎
  • Journal Title: Modern Physician Volume: vol 32 No.8 Pages: 1022-1024
• [Journal Article] A single nucleotide polymorphism within DUSP9 is associated with susceptibility to type 2 diabetes in a Japanese population. (2012)
  • Author(s): Hisashi Fukuda
  • Journal Title: PLoS One Volume: 7 Issue: 9 Pages: e46263-e46263
  • DOI: 10.1371/journal.pone.0046263
  • Peer Reviewed
• [Journal Article] Genetic variants at CDC123/CAMK1D and SPEY2 are associated with susceptibility to type 2 diabetes in the Japanese population (2011)
  • Author(s): Imamura, et al
  • Journal Title: Diabetologia Volume: 54 Issue: 12 Pages: 3071-3077
  • DOI: 10.1007/s00125-011-2293-3
  • Peer Reviewed
• [Journal Article] Genetics of type 2 diabetes: the GWAS era and future perspectives [Review] (2011)
  • Author(s): Imamura M, Maeda S
  • Journal Title: Endocrine Journal Volume: 58 Issue: 9 Pages: 723-739
  • DOI: 10.1507/endocrj.EJ11-0113
  • NAID: 130004443740
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Presentation] Usefulness of genetic risk score constructed from 49 loci for type 2 diabetes in Japanese individuals (2013)
  • Author(s): Minako Imamura
  • Organizer: 49th EASD Annual Meeting
  • Place of Presentation: Barcelona
• [Presentation] Usefulness of genetic risk score constructed from 47 loci for type 2 diabetes in non-obese, older Japanese individuals (2013)
  • Author(s): Minako Imamura
  • Organizer: American Diabetes Association's 73rd Scientific Sessions
  • Place of Presentation: Chicago, Illinois
• [Presentation] 2型糖尿病疾患感受性遺伝子領域55領域の日本人集団における検討 (2013)
  • Author(s): 今村美菜子
  • Organizer: 第56回日本糖尿病学会年次学術集会
  • Place of Presentation: 熊本
• [Presentation] 2型糖尿病疾患感受性遺伝子領域55領域の日本人集団における検討 (2013)
  • Author(s): 今村美菜子, 福田尚志, 岩田実, 綿田裕孝, 前川聡, 広瀬寛,
  • Organizer: 第56回日本糖尿病学会年次学術集会
  • Place of Presentation: メルパルク熊本(熊本市）
• [Presentation] Usefulness of genetic risk score constructed from 47 loci for type 2 diabetes in non-obese, older Japanese individuals (2013)
  • Author(s): Minako Imamura, Minoru Iwata, Hiroshi Maegawa, Hirotaka Watada, Hiroshi Hirose, Yasushi Tanaka, Kazuyuki Tobe, Kohei Kaku, Atsunori Kashiwagi, Ryuso Kawamori, Shiro Maeda
  • Organizer: American Diabetes Association’s 73rd Scientific Sessions
  • Place of Presentation: McCormick Place、Chicago, Illinois, USA
• [Presentation] Usefulness of genetic risk score constructed from 49 loci for type 2 diabetes in Japanese individuals (2013)
  • Author(s): Minako Imamura, Minoru Iwata, Hiroshi Maegawa, Hirotaka Watada, Hiroshi Hirose, Yasushi Tanaka, Kazuyuki Tobe, Kohei Kaku, Atsunori Kashiwagi, Ryuso Kawamori, Shiro Maeda
  • Organizer: 49th EASD Annual Meeting
  • Place of Presentation: Fira Gran Via （Balcerona) Spain
• [Presentation] Imputationに基づくゲノムワイド関連解析による2型糖尿病疾患感受性遺伝子領域の同定 (2012)
  • Author(s): 今村美菜子
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: 横浜
• [Presentation] Imputationに基づくゲノムワイド関連解析による2型糖尿病疾患感受性遺伝子領域の同定 (2012)
  • Author(s): 今村美菜子, 前田士郎, 山内敏正, 原一雄, 安田一基, 綿田裕孝, 前川聡, 岩田実, 広瀬寛, 加来浩平, 伊藤千賀子, 田中逸, 戸辺一之, 柏木厚典, 河盛隆造, 春日雅人, 中村祐輔, 門脇孝
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: パシフィコ横浜 （横浜市）
• [Presentation] Genetic variations in DUSP9 locus and CMIP locus are associated with type 2 diabetes in a Japanese population (2012)
  • Author(s): M. Imamura, H. Fukuda, M. Iwata, H. Maegawa, H. Watada, H. Hirose, Y. Tanaka, K. Tobe, K. Kaku, A. Kashiwagi, R. Kawamori, S. Maeda
  • Organizer: 48th EASD annual meetings
  • Place of Presentation: Messe Berlin, Berlin, Germany
• [Presentation] 2型糖尿病感受性候補領域33領域の日本人集団における検証 (2011)
  • Author(s): 今村美菜子
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌
• [Presentation] Rs10906115 in CDC123/CAMK1D locus and rs1359790 near SPRY2 are associated with susceptibility to type 2 diabetes in a Japanese population (2011)
  • Author(s): Minako Imamura, Minoru Iwata，Michiaki Kubo, Yusuke Nakamura, Naoyuki Kamatani, Kazuyuki Tobe, Shiro Maeda
  • Organizer: American Diabetes Association 71st scientific sessions
  • Place of Presentation: san diego USA
• [Presentation] Rs10906115 in CDC123/CAMK1D locus and rs1359790 near SPRY2 are associated with susceptibility to type 2 diabetes in a Japanese population (2011)
  • Author(s): Minako Imamura, Minoru Iwata, Hiroshi Maegawa, Hirotaka Watada, Hiroshi Hirose, Yasushi Tanaka, Kazuyuki Tobe, Kohei Kaku, Atsunori Kashiwagi, Ryuzo Kawamori, Yusuke Nakamura, Shiro Maeda,
  • Organizer: 47th EASD annual meetings
  • Place of Presentation: Lisbon Portugal
• [Presentation] 中国人GWASで新たに同定された2型糖尿病疾患感受性遺伝子座の日本人集団での検証 (2011)
  • Author(s): 今村美菜子 岩田実 前川 聡、綿田 裕孝 広瀬寛 田中逸 戸辺一之 加来浩平 柏木厚典 河盛 隆造 中村祐輔
  • Organizer: 日本人類遺伝学会第56回大会
  • Place of Presentation: 千葉
• [Remarks] 独立行政法人理化学研究所, 統合生命医科学研究センター, 腎代謝内分泌疾患研究チームホームページ
  • URL: http://www.riken.jp/research/labs/ims/endocrinol_metab_kidney_dis/
• [Remarks] アウトリーチ活動サイエンスカフェ講師今村美菜子タイトル"糖尿病になりやすい体質ってなんだろう?"2012年8月26日(日)神奈川県立川崎図書館2階ホール
  • URL: http://www.klnet.pref.kanagawa.jp/kawasaki/information/science_cafe031.htm