Detection for miRNA dysregulation in CML
Project/Area Number |
23591365
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
|
Research Institution | Akita University |
Principal Investigator |
TAKAHASHI Naoto 秋田大学, 医学(系)研究科(研究院), 講師 (80344753)
|
Co-Investigator(Kenkyū-buntansha) |
TAGAWA Hiroyuki 秋田大学, 大学院医学系研究科, 講師 (30373492)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
|
Keywords | CML / miRNA / miR-200c / Bmi-1 / microRNA / 骨髄性白血病 / 慢性骨髄性白血病 |
Research Abstract |
To detect specific miRNA in cancer stem cell of chromic myelogenious leukemia (CML), we established CML mice (B6) which can develop CML. We got cancer stem cells from B6 mice and examined miRNA expression from KLS population. We found that several miRNA were up-or down regulated in these mice. For instance, down regulation of miR-203,miR-200c, miR-26,miR-21,miR-2105, and miR-181. In this time we are examining targets of these miRNAs. We identified Bmi-1 is possible target of miR-200c in CML stem cells. And we fort her demonstrated that bortezomib was effective for diminishing CML stem cells by targeting both Bmi-1 and miR-200c.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Bortezomib reduces the tumorigenicity of multiple myeloma via downregulation of upregulated targets in clonogenic side population cells2013
Author(s)
Nara M, Teshima K, Watanabe A, Ito M, Iwamoto K, Kitabayashi A, Kume M, Hatano Y, Takahashi N, Iida S, Sawada K, Tagawa H
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Journal Title
Related Report
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[Journal Article] Bortezomib reduces the tumorigenicity of multiple myeloma via downregulation of upregulated targets in clonogenic side population cells2013
Author(s)
Nara M, Teshima K, Watanabe A, Ito M, Iwamoto K, Kitabayashi A, Kume M, Hatano Y, Takahashi N, Iida S, Sawada K, Tagawa H.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Enucleation of human erythroblasts involves non-muscle myosin IIB2012
Author(s)
Ubukawa K, Guo YM, Takahashi M, Hirokawa M, Michishita Y, Nara M, Tagawa H, Takahashi N, Komatsuda A, Nunomura W, Takakuwa Y, Sawada K
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Journal Title
Blood
Volume: 119
Pages: 1036-44
Related Report
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[Journal Article] A synthetic double-stranded RNA, poly I : C, induces a rapid apoptosis of human CD34(+) cells2012
Author(s)
Liu J, Guo YM, Hirokawa M, Iwamoto K, Ubukawa K, Michishita Y, Fujishima N, Tagawa H, Takahashi N, Xiao W, Yamashita J, Ohteki T, Sawada K
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Journal Title
Exp Hematol
Volume: 40
Pages: 330-41
Related Report
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[Journal Article] False-positive human immunodeficiency virus antibody test and autoimmune hemolytic anemia in a patient with angioimmunoblastic T-cell lymphoma2011
Author(s)
Shida S, Takahashi N, Fujishima N, Kameoka Y, Nara M, Fujishima M, Saitoh H, Tagawa H, Hirokawa M, Ichinohasama R, Sawada K
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Journal Title
Intern Med
Volume: 50(20)
Pages: 2383-7
NAID
Related Report
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[Journal Article] Age-associated alteration ofγδT-cell repertoire and different profiles of activation-induced death of Vδ1 and Vδ2 T cells2011
Author(s)
Michishita Y, Hirokawa M, Guo YM, Abe Y, Liu J, Ubukawa K, Fujishima N, Fujishima M, Yoshioka T, Kameoka Y, Saito H, Tagawa H, Takahashi N, Sawada K
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Journal Title
Int J Hematol
Volume: 94
Pages: 230-40
Related Report
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[Journal Article] The role of microRNA-150 as a tumor suppressor in malignant lymphoma2011
Author(s)
Watanabe A, Tagawa H, Yamashita J, Teshima K, NaraM, Iwamoto K, Kume M, Kameoka Y, Takahashi N, Nakagawa T, Shimizu N, Sawada K
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Journal Title
Leukemia
Volume: 25
Pages: 1324-34
Related Report
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[Journal Article] Drug interaction of (S)-warfarin, and not (R)-warfarin, with itraconazole in a hematopoietic stem cell transplant recipient2011
Author(s)
Miura M, Takahashi N, Kanno S, Kato S, Nara M, Itoh M, Saitoh H, Yoshioka T, Kameoka Y, Fujishima N, Tagawa H, Hirokawa M, Sawada K
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Journal Title
Clin Chim Acta
Volume: 9;412
Pages: 2002-6
Related Report
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[Journal Article] Successful treatment of necrotizing fasciitis in an upper extremity caused by Clostridium perfringens after bone marrow transplantation2011
Author(s)
Ito M, Takahashi N, Saitoh H, Shida S, Nagao T, Kume M, Kameoka Y, Tagawa H, Fujishima N, Hirokawa M, Tazawa H, Minato T, Yamada S, Sawada K.
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Journal Title
Intern Med
Volume: 50
Pages: 2213-7
NAID
Related Report
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