The analysis of leukemia stem cell using iPS cell technology
| Project/Area Number |
23591369
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kansai Medical University (2013)
The University of Tokyo (2011-2012) |
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Principal Investigator |
KUMANO keiki 関西医科大学, 医学部, 准教授 (90396721)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMIKUBO Yasuhiko 京都大学, 医学部, 准教授 (60548527)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | iPS細胞 / 白血病幹細胞 / iPS cells |
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| Research Abstract |
Induced pluripotent stem cells (iPSCs) can be generated by the expression of defined transcription factors not only from normal tissue, but also from malignant cells. Bone marrow cells from a chronic myelogenous leukemia (CML), myelofibrosis (MF), essential thrombocythemia (ET) patient were reprogrammed by introducing the transcription factors Oct3/4, Sox2, KLF4, and c-myc. Then we differentiated them into hematopoietic progenitors. They showed the hematopoietic marker CD45 and immature marker CD34. CML derived hematopoietic cells recovered the sensitivity to imatinib, which recapitulated the feature of initial CML disease. mRNA levels of IL-8, one of candidate cytokines related to the pathogenesis of myelofibrosis, was expressed elevated predominantly in megakaryocytes derived from MF-iPSCs. Thus, this system can act as a new platform for the elucidation of the disease mechanism and drug screening.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Generation of induced pluripotent stem cells derived from primary and secondary myelofibrosis patient samples2014
Author(s)
Hosoi M, Kumano K, Taoka K, Arai S, Kataoka K, Ueda K, Kamikubo Y, Takayama N, Otsu M, Eto K, Nakauchi H, Kurokawa M
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Journal Title
Exp Hematol
Volume: (in press)
Issue: 9
Pages: 816-25
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The IL-2/CD25 Axis Maintains Distinct Subsets of Chronic M yeloid Leukemia-initiating Cells.2014
Author(s)
Kobayashi CI, Takubo K, Kobayashi H, Nakamura-Ishizu A, Honda H, Kataoka K, Kumano K, Akiyama H, Sudo T, Kurokawa M, Suda T.
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Journal Title
Blood
Volume: 123
Issue: 16
Pages: 2540-2549
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The shortest isoform of C/EBPβ, Liver inhibitory protein (LIP) , collaborates with Evi1 to induce AML in a mouse BMT model2013
Author(s)
Okochi, N., Yoshimi, A., Sato, T., Ikeda, T., Kumano, K., Taoka, K., Sato, Y., Shinohara, A., Tsuruta, A., Masuda, A., Yokota, H., Yatomi, Y., Takahashi, K., Kitaura, J., Kitamura, T. and Kurokawa, M
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Journal Title
Blood
Volume: 121
Issue: 20
Pages: 4142-4155
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Multiple phosphorylation sites are important for RUNX1 activity in early hematopoiesis and T-cell differentiation.2012
Author(s)
Yoshimi M, Goyama S, Kawazu M, Nakagawa M, Ichikawa M, Imai Y, Kumano K, Asai T, Mulloy JC, Kraft AS, Takahashi T, Shirafuji N, Kurokawa M.
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Journal Title
Eur J Immunol.
Volume: 42
Issue: 4
Pages: 1044-1050
DOI
Related Report
Peer Reviewed
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[Journal Article] Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine2011
Author(s)
Sakata-Yanagimoto M, Sakai T, Miyake Y, Saito T, Maruyama H, Morishita Y, Nakagami-Yamaguchi E, Kumano K, Yagita H, Fukayama M, Ogawa S, Kurokawa M, Yasutomo K, Chiba S
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Journal Title
Blood
Volume: 117
Issue: 1
Pages: 128-134
DOI
NAID
Related Report
Peer Reviewed
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