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The role of multimerin1 and VacA, an exotoxin released by H. pylori on ITP.

Research Project

Project/Area Number 23591378
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hematology
Research InstitutionUniversity of Yamanashi

Principal Investigator

OZAKI Yukio  山梨大学, 医学工学総合研究部, 教授 (30134539)

Co-Investigator(Kenkyū-buntansha) SATOH Kaneo  山梨大学, 医学部, 助手 (20242662)
TAKANO Katsuhiro  山梨大学, 医学部附属病院, 助教 (60382925)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsVacA / Helicobactor pylori / 特発性血小板減少性紫斑病 / multimerin1 / platelet / 血液内科学 / multimerin 1 / 特発性血小板減少症 / ヘリコバクターピロリ / マルチメリン / 毒素
Research Abstract

Platelets were activated under the infection with H. pylori in human and mice. We investigated the role of VacA, an exotoxin released by H. pylori in this context. Acid-activated VacA, but not heated VacA, induced platelet CD62P expression. However, VacA reacted with none of the alleged VacA receptors present on platelet membranes. We therefore analyzed VacA associated proteins obtained through VacA affinity chromatography, using MALDI-TOF-MS. Multimerin1 was detected in two consecutive experiments, as the binding protein for VacA. Plasmon resonance confirmed their binding, and dot print analysis revealed that the peptide sequence AA 321-340 of multimerin 1 is the binding site for VacA. In conclusion, we propose a new interaction between multimerin1 and VacA , which may give another insight into H. pylori-induced platelet activations under H. pylori infection.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2014 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] VacA, the vacuolating cytotoxin of Helicobacter pylori, binds to multimerin 1 on human platelets.2013

    • Author(s)
      Satoh K, Hirayama T, Takano K, Suzuki-Inoue K, Sato T, Ohta M, Nakagomi J, Ozaki Y
    • Journal Title

      Thromb J.

      Volume: 11 Issue: 1 Pages: 23-30

    • DOI

      10.1186/1477-9560-11-23

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Helicobacter pylori産生毒素Vacuolating toxin Aの結合タンパクに関する解析2014

    • Author(s)
      佐藤金夫、平山壽哉、高野勝弘、井上克枝、多田正人、尾崎由基男
    • Organizer
      第36回日本血栓止血学会
    • Place of Presentation
      大阪国際交流センター(大阪府)
    • Year and Date
      2014-05-31
    • Related Report
      2013 Final Research Report
  • [Presentation] Helicobacter pylori産生毒素Vacuolating toxin Aの結合タンパクに関する解析2014

    • Author(s)
      佐藤金夫、平山壽哉、高野勝弘、井上克枝、多田正人、尾崎由基男
    • Organizer
      第36回 日本血栓止血学会
    • Place of Presentation
      大阪国際交流センター (大阪府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] VacA, the vacuolating cytotoxin of Helicobacter pylori, binds to multimerin 1 associated with platelet membranes2013

    • Author(s)
      Satoh K, Hirayama T, Takano K, Suzuki-Inoue K, Sato T, Ohta M, Nakagomi J, Ozaki Y
    • Organizer
      XXIVth International Society of thrombosis and Haemostasis
    • Place of Presentation
      Amsterdam RAI (Netherlands)
    • Year and Date
      2013-07-02
    • Related Report
      2013 Final Research Report
  • [Presentation] VacA, the vacuolating cytotoxin of Helicobacter pylori, binds to multimerin 1 associated with platelet membranes.2013

    • Author(s)
      K. Satoh, T. Hirayama, K. Takano, T. Sato, Y. Ozaki.
    • Organizer
      XXIVth International society on thrombosis and haemostasis
    • Place of Presentation
      Amsterdam RAI (Netherlands)
    • Related Report
      2013 Annual Research Report
  • [Presentation] The role of VacA, an exotoxin in Helicobacter pylori-related ITP2012

    • Author(s)
      Yukio Ozaki
    • Organizer
      7th Asian-Pacific Society on Thrombosis and Hemostasis
    • Place of Presentation
      Melbourne Convention Exhibition Centre (Australia)
    • Year and Date
      2012-10-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Helicobacter pylori infection and immune thrombocytopenic purpura: new mechanism?2012

    • Author(s)
      尾崎由基男
    • Organizer
      オーストラリア血液学会
    • Place of Presentation
      メルボルン、オーストラリア
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks] ホームページ等, 山梨大学大学院医学工学総合研究部臨床検査医学講座 研究紹介

    • URL

      http://www.med.yamanashi.ac.jp/clinical/clin0lab/cn6/cn22/index2.1.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 山梨大学大学院医学工学総合研究部 臨床検査医学講座

    • URL

      http://www.med.yamanashi.ac.jp/clinical/clin0lab/cn6/cn22/index2.1.html

    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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