| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
We examined effects of FGF2 on interaction between leukemia cells and its microenvironment. Significantly fewer AML cells were recovered from cocultures with murine stromal monolayers treated with FGF2 than from control. FGF2 also facilitate the sensitivity of CML cells toward imatinib in the coculture. Accordingly, AML cells in cell cycle quiescent are less in FGF2-treated monolayers than control. We next treated human AML mouse model with Ara-C in combination w/wo FGF2. BM sections from FGF2-treated mice displayed thickened bone trabeculae and an increased number of leukemia cells compared to control. FGF2 did not help leukemia cells survive from Ara-C in the spleen. These observations suggest that the endosteal region consisting of osteoblasts is important for leukemia cells to survive from chemotherapy, and that FGF2 favors leukemia cell in the BM via promoting osteoblast functions.
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