Identification of multiple myeloma stem cells and development of antibody therapy targeting them
| Project/Area Number |
23591386
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
HOSEN Naoki 大阪大学, 医学(系)研究科(研究院), 准教授 (10456923)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 多発性骨髄腫 / がん幹細胞 / 抗体療法 / l抗体療法 / 抗体医療 |
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| Research Abstract |
We have already shown that "myeloma-initiating cells", which are the origin of the whole myeloma cells, exist within the CD19+CD38- plasma cell population. In this study, we aimed to identify cell surface antigens expressed on "myeloma-initiating cells" and target them with monoclonal antibody.Thousands of monoclonal antibodies taht bind to several myeloma cell lines were first established. Next, we screened for antibodies that inhibit the support by bone marrow stromal cells, and found that MM1 antibody is a good candidate. Furthermore, we searched for antigens specifically expressed on myeloma cells but not on normal hematopoietic cells. We have identified several antibodies as candidates, and also isolated antigens recognized by them by using cDNA expression cloning. Now we are examining whether these antibodies can eliminate "myeloma-initiating cells" in vivo using xenograft models.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] CD138-negative clonogenic cells are plasma cells but not B cells in some multiple myeloma patients2012
Author(s)
Hosen N, Matsuoka Y, Kishida S, Nakata J, Mizutani Y, Hasegawa K, Mugitani A, Ichihara H, Aoyama Y, Nishida S, Tsuboi A, Fujiki F, Tatsumi N, Nakajima H, Hino M, Kimura T, Yata K, Abe M, Oka Y, Oji Y, Kumanogoh A, Sugiyama H.
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Journal Title
Leukemia
Volume: (in press)
Issue: 9
Pages: 2135-2141
DOI
Related Report
Peer Reviewed
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