Development of novel anti-tumor drugs inhibiting Anamorsin and Picot binding
| Project/Area Number |
23591387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
SAITOH Norimitsu 大阪大学, 大学院医学(系)研究科(研究院), 特任助教(常勤) (30597399)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBAYAMA Hirohiko 大阪大学, 大学院医学系研究科, 講師 (60346202)
KANAKURA Yuzuru 大阪大学, 大学院医学系研究科, 教授 (20177489)
ORITANI Kenji 大阪大学, 大学院医学系研究科, 准教授 (70324762)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Anamorsin / Picot / 鉄硫黄クラスター / 細胞内シグナル伝達 / Ras / Bリンパ球 / LPS / B細胞性リンパ腫 / PICOT / 鉄・硫黄クラスター / トランスジェニック / ERK1/2 / IkB / P53 / PKC / P38MAPK |
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| Research Abstract |
Anamorsin (AM) was originally isolated as a molecule that conferred resistance to apoptosis. AM deficient mice die during late gestation; AM is indispensable for embryo growth and hematopoiesis. Furthermore, our previous study showed AM overexpression in approximately 30% of B cell neoplasm cases. In this work, we focused on B cells of AM transgenic (Tg) mice. LPS-stimulated proliferation of AM Tg B cells was decreased compared with WT mice. Phosphorylation of Erk1/2 and IKBa in LPS-stimulated AM Tg B cells was also decreased. Upstream analysis based on the results of DNA micro array comparing AM Tg and WT B cells stimulated with or without LPS suggested that Ras activation was decreased in LPS-stimulated AM Tg B cells. We also confirmed impairment of LPS induced Ras activation in AM Tg mice by Ras assay. Our findings indicate the possibility that AM plays a role of negative regulation in LPS signaling pathway through the inhibition of Ras activation, which is a novel function of AM.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] The Satb1 protein directs hematopoietic stem cell differentiation toward lymphoid lineages.2013
Author(s)
Satoh Y, Yokota T, Sudo T, Kondo M, Lai A, Kincade PW, Kouro T, Iida R, Kokame K, Miyata T, Habuchi Y, Matsui K, Tanaka H, Matsumura I, Oritani K, Kohwi-Shigematsu T, Kanakura Y.
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Journal Title
Immunity
Volume: 38
Issue: 6
Pages: 1105-1115
DOI
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Peer Reviewed
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[Presentation] MS4A3発現はヒト造血における早期骨髄球系分化の指標となる
Author(s)
石橋知彦, 横田貴史, 佐藤友亮, 数藤孝雄, 一井倫子, 柴山浩彦, 江副幸子, 谷村 朗, 藤田奈津子, 松井敬子, 濱中有理, 土居由貴子, 織谷健司, 金倉 譲
Organizer
第75回日本血液学会学術集会
Place of Presentation
ロイトン札幌（札幌、北海道）
Related Report
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[Presentation] Endothelial Cell-Selective Adhesion Molecule Marks Human Hematopoietic Stem Cells Regardless Of The HSC Sources
Author(s)
Ishibashi T, Yokota T, Ichii M, Satoh Y, Sudo T, Doi Y, Fujita N, Nagate Y, Hamanaka Y, Matsui K, Tanimura A, Saitoh N, Ezoe S, Shibayama H, Kincade PW, Oritani1 K, Kanakura Y
Organizer
The American Society of Hematology 55th Annual Meeting
Place of Presentation
Ernest N. Morial Convention Center, New Orleans, LA, USA
Related Report
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