Analysis of myeloma stem cells and their niche

• Project/Area Number: 23591404
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Kyoto Pharmaceutical University(2012-2013); Kyoto Prefectural University of Medicine(2011)
• Principal Investigator: ASHIHARA Eishi 京都薬科大学, 薬学部, 教授 (70275197)
• Co-Investigator(Kenkyū-buntansha): HIRAI Hideyo 京都大学, 大学院医学研究科, 助教 (50315933)
• Project Period (FY): 2011 – 2013
• Project Status: Completed(Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost : ¥3,800,000、Indirect Cost : ¥1,140,000); Fiscal Year 2013 : ¥1,430,000 (Direct Cost : ¥1,100,000、Indirect Cost : ¥330,000); Fiscal Year 2012 : ¥1,950,000 (Direct Cost : ¥1,500,000、Indirect Cost : ¥450,000); Fiscal Year 2011 : ¥1,560,000 (Direct Cost : ¥1,200,000、Indirect Cost : ¥360,000)
• Keywords: 多発性骨髄腫 / 低酸素 / 骨髄微小環境 / がん幹細胞 / 低酸素環境 / TGF-β / がん微小環境

Research Abstract

We investigated the characteristics of hypoxia-adapted MM (HA-MM) cells. Irradiated NOD/SCID mice were inoculated with AMO-1 MM cells. After 2 or 4 weeks transplantation, the inoculated MM cells were positive for pimonidazole. These observations suggested that MM cells are hypoxic in the BM. We then established HA-MM cell lines that can continue to proliferate in hypoxic conditions (1% O2) for more than six months. The G0 fraction cells significantly increased in HA-MM cells compared with those in the parental MM cells. The survival durations of mice transplanted with HA-MM cells were significantly shorter than that of mice transplanted with parental MM cells. Stem cell markers such as Sox2, Oct3, and Nanog mRNA transcripts increased in the HA-MM AMO-1 cells. Furthermore, phosphorylated Smad2 expression was increased in HA-AMO-1 cells. These findings suggest that HA-MM cells possess stem cell-like characters and may provide a useful model to investigate the mechanism of MM stem cells.

Research Products

• [Journal Article] Functional assessment of the mutational effects of human IRAK4 and MyD88 genes. (2014)
  • Author(s): Yamamoto T, Tsutsumi N, Tochio H, Ohnishi H, Kubota K, Kato Z, Shirakawa M, Kondo N
  • Journal Title: Mol Immunol. Volume : 58 Pages : 66-76
  • DOI: 10.1039/c3cc39205h
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Myeloid-Derived Suppressor Cells Play Crucial Roles in the Regulation of Mouse Collagen-Induced Arthritis. (2013)
  • Author(s): 1. Fujii W, Ashihara E, Hirai H, Nagahara H, Kajitani N, Fujioka K, Murakami K, Seno T, Yamamoto A, Ishino H, Kohno M, Maekawa T, Kawahito Y.
  • Journal Title: J Immunol Volume : 191 Pages : 1073-1081
  • DOI: 10.4049/jimmunol.1203535
  • Peer Reviewed
• [Journal Article] C/EBPβ promotes BCR-ABL-mediated myeloid expansion and leukemic stem cell exhaustion. (2013)
  • Journal Title: Leukemia Volume : 27 Issue : 3 Pages : 619-628
  • Publisher: Nature Publishing Group
  • DOI: 10.1038/leu.2012.258
  • PMID: 22948537
  • Peer Reviewed
• [Journal Article] Effectiveness of sulforaphane as a radiosensitizer for murine osteosarcoma cells (2013)
  • Author(s): Y. Sawai, H. Murata, M. Horii, K. Koto,T. Matsui, N. Horie, Y. Tsuji, E. Ashihara, T. Maekawa, T. Kubo, S. Fushiki
  • Journal Title: Oncol Rep Volume : 29 Pages : 941-945
  • DOI: 10.3892/or.2012.2195
  • Peer Reviewed
• [Journal Article] Zoledronic acid significantly enhances radiation induced apoptosis against human fibrosarcoma cells by inhibiting radioadaptive signaling. (2013)
  • Author(s): 8. Koto K, Murata H, Kimura S, Sawai Y, Horie N, Matsui T, Ryu K, Ashihara E, Maekawa T, Kubo T, Fushiki S.
  • Journal Title: Int J Oncol Volume : 42 Pages : 525-534
  • DOI: 10.3892/ijo.2012.1735
  • Peer Reviewed
• [Journal Article] Role of IL-22- and TNF-α-producing Th22 cells in uveitis patients with Behcet's disease (2013)
  • Author(s): Sugita S, Kawazoe Y, Imai A, Kawaguchi T, Horie S, Keino H, Takahashi M, Mochizuki M
  • Journal Title: J Immunol Volume : 190 Pages : 5799-808
  • DOI: 10.4049/jimmunol.1103007
  • Peer Reviewed
• [Journal Article] Therapeutic effects of human mesenchymal and hematopoietic stem cells on rotenone-treated parkinsonian mice (2013)
  • Author(s): Inden M, Takata K, Nishimura K, Kitamura Y, Ashihara E, Yoshimoto K, Ariga H, Honmou O, Shimohama S
  • Journal Title: Journal of Neuroscience Research Volume : 91 Pages : 62-72
  • DOI: 10.1002/jnr.23128
  • Peer Reviewed
• [Journal Article] 非小細胞性肺がん患者に対する簡易懸濁法を用いたエルロチニブ投与に関する検討． (2013)
  • Author(s): 高田哲也、池見泰明、福土将秀、杉本充弘、石橋直哉、小林政彦、矢野育子、金永学、三嶋理晃、芦原英司、松原和夫.
  • Journal Title: 医療薬学 Volume : 39 Pages : 565-570
  • Peer Reviewed
• [Journal Article] Cyclic AMP Responsive Element Binding Proteins Are Involved in 'Emergency' Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β. (2013)
  • Journal Title: PloS one Volume : 8 Issue : 1
  • Publisher: Public Library of Science
  • DOI: 10.1371/journal.pone.0054862
  • PMID: 23382991
  • Year and Date: 2013-01-30
  • Peer Reviewed
• [Journal Article] An inhibitor of Na+/H+ exchanger (NHE), ethyl-isopropyl amiloride (EIPA), diminished proliferation of MNK28 human gastric cancer cells by decreasing the cytosolic Cl- concentration via DIDS-sensitive pathways. (2012)
  • Author(s): Hosogi S
  • Journal Title: Cell Physiol Biochem Volume : 30 Pages : 1241-1253
  • DOI: 10.1159/000343315
  • Peer Reviewed
• [Journal Article] Effects of low-intensity pulsed ultrasound (LIPUS) on osteosarcoma and cancer cells. (2012)
  • Author(s): Sawai Y
  • Journal Title: Oncol Rep Volume : 28 Pages : 481-486
  • DOI: 10.3892/or.2012.1816
  • Peer Reviewed
• [Journal Article] ranslational Research of Photodynamic Therapy with Acridine Orange which Targets Cancer Acidity. (2012)
  • Author(s): Kusuzaki K
  • Journal Title: Curr Pharm Des Volume : 18 Pages : 1414-1420
  • Peer Reviewed
• [Journal Article] AV-65, a novel Wnt/βcatenin signal inhibitor, successfully suppresses progression of multiple myeloma in a mouse model (2011)
  • Author(s): Yao H, Ashihara E, Yokota A, Shimazaki C. (他13名)
  • Journal Title: Blood Cancer J Volume : 1
  • DOI: 10.1038/bjc.2011.41
  • Peer Reviewed
• [Journal Article] Targeting the Wnt/β-catenin signaling pathway in human cancers (2011)
  • Author(s): Yao H, Ashihara E, Maekawa T
  • Journal Title: Expert Opinion on Therapeutic Targets Volume : 15 Pages : 873-887
  • DOI: 10.1517/14728222.2011.577418
  • Peer Reviewed
• [Journal Article] Galectin-3(Gal-3) induced by leukemia microenvironment promotes drug resistance and bone marrow lodgment in chronic myelogenous leukemia (2011)
  • Author(s): Yamamoto-Sugitani M, Kuroda J, Ashihara E, Nagoshi H, Kobayashi T, Matsumoto Y, Sasaki N, Shimura Y, Kiyota M, Nakayama R, Akaji K, Taki T, Uoshima N, Kobayashi Y, Horiike S, Maekawa T, Taniwaki M
  • Journal Title: Proc Natl Acad Sci U S A Volume : 108 Pages : 17468-17473
  • DOI: 10.1073/pnas.1111138108
  • Peer Reviewed
• [Journal Article] Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients (2011)
  • Author(s): Tanaka, R., Kimura, S., Ashihara, E., Yoshimura, M., Takahashi, N., Wakita, H., Itoh, K., Nishiwaki, K., Suzuki, K., Nagao, R., Yao, H., Hayashi, Y., Satake, S., Hirai, H., Sawada, K-I., Ottmann, O. G., Melo, J. V.
  • Journal Title: Cancer Letter Volume : 312(2) Pages : 228-34
  • DOI: 10.1016/j.canlet.2011.08.009
  • Peer Reviewed
• [Journal Article] Combined effects of novel heat shock protein 90 inhibitor NVP-AUY922 and nilotinib in a random mutagenesis screen (2011)
  • Author(s): Tauchi T, Okabe S, Ashihara E. (他3名)
  • Journal Title: Oncogene Volume : 30 Pages : 2789-2797
  • DOI: 10.1038/onc.2011.3
  • Peer Reviewed
• [Journal Article] Rakicidin A effectively induces apoptosis in hypoxia adapted Bcr-Abl positive leukemic cells (2011)
  • Author(s): Takeuchi M, Ashihara E, Maekawa T, et al
  • Journal Title: Cancer Sci Volume : 102 Pages : 591-6
  • DOI: 10.1111/j.1349-7006.2010.01813.x
  • Peer Reviewed
• [Journal Article] Galection-9 ameliorates acute Graft-versus-host disease through the induction of T-cell apoptosis (2011)
  • Author(s): Sakai K, Ashihara E, Maekawa T, et al
  • Journal Title: Eur J Immunol Volume : 41 Pages : 67-75
  • DOI: 10.1002/eji.200939931
  • Peer Reviewed
• [Journal Article] Targeting the Wnt/beta-catenin signaling pathway in human cancers. (2011)
  • Author(s): Yao H, Ashihara E, Maekawa T.
  • Journal Title: Expert Opinion on Therapeutic Targets Volume : 15 Pages : 873-87
  • Peer Reviewed
• [Journal Article] Galectin-3 induced by leukemia microenvironment promotes drug resistance and bone marrow lodgment in chronic myelogenous leukemia. (2011)
  • Author(s): Yamamoto-Sugitani M, Kuroda J, Ashihara E,et al.
  • Journal Title: Proc Natl Acad Sci USA Volume : 108 Pages : 17468-73
  • Peer Reviewed
• [Journal Article] Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients. (2011)
  • Author(s): Tanaka R, Ashihara E, Maekawa T, et al.
  • Journal Title: Cancer Lett Volume : 312 Pages : 228-34
  • Peer Reviewed
• [Journal Article] Growth inhibition of imatinib-resistant CML cells with the T315I mutation and hypoxia-adaptation by AV65, a novel Wnt/beta-catenin signaling inhibitor. (2011)
  • Author(s): Nagao R, Ashihara E, Maekawa T, et al.
  • Journal Title: Cancer Lett Volume : 312 Pages : 91-100
  • Peer Reviewed
• [Journal Article] Rakicidin A effectively induces apoptosis in hypoxia adapted Bcr-Abl positive leukemic cells. (2011)
  • Author(s): Takeuchi M, Ashihara E, Maekawa T,et al.
  • Journal Title: Cancer Sci Volume : 102 Pages : 591-6
  • Peer Reviewed
• [Journal Article] Galection-9 ameliorates acute Graft-versus-host disease through the induction of T-cell apoptosis.. (2011)
  • Author(s): Sakai K, Ashihara E, Maekawa T,et al.
  • Journal Title: Eur J Immunol Volume : 39 Pages : 67-75
  • Peer Reviewed
• [Journal Article] RNA Interference against Polo-like kinase-1 in advanced non-small cell lung cancers. (2011)
  • Author(s): Kawata E, Ashihara E, Maekawa T.
  • Journal Title: J Clin Bioinformatics Volume : 1 Pages : 6-6
  • Peer Reviewed
• [Journal Article] アンチセンス研究入門46 RNA干渉のがん治療への応用． (2011)
  • Author(s): 芦原英司
  • Journal Title: Antisense Volume : 15 Pages : 31-47
• [Journal Article] Growth inhibition of imatinib-resistant CML cells with the T315I mutation and hypoxia-adaptation by AV65 - a novel Wnt/β-catenin signaling inhibitor. (2011)
  • Journal Title: Cancer letters Volume : 312 Issue : 1 Pages : 91-100
  • Publisher: Elsevier Ireland Ltd.
  • DOI: 10.1016/j.canlet.2011.08.002
  • PMID: 21906872
  • Year and Date: 2011-12-15
  • Peer Reviewed
• [Journal Article] Parathyroid hormone enhances hematopoietic expansion via upregulation of cadherin-11 in bone marrow mesenchymal stromal cells
  • Author(s): Yao H, Miura Y, Ashihara E, Hirai H, et al. (他11名)
  • Journal Title: Stem Cells Volume : (in press)
  • Peer Reviewed
• [Presentation] Hypoxia-adapted myeloma cells possess stem cell-like character (2013)
  • Author(s): Ashihara E, Hirai H, et al
  • Organizer: The 55^<th> Annual Meeting of American Society of Hematology
  • Place of Presentation: New Orleans, USA
• [Presentation] Direct interaction with bone marrow mesenvhymal stromal/stem cells is required for hematopoietic expansion by parathyroid hormone (2013)
  • Author(s): Yao H, Miura Y, Ashihara E, et al
  • Organizer: The 55^<th> Annual Meeting of American Society of Hematology
  • Place of Presentation: New Orleans, USA
• [Presentation] Expression of C/EBPβis involved in the exhaustion of hematopoietic stem cells through cell-intrinsic and cell-extrinsic mechanisms (2013)
  • Author(s): Tamura A, Hirai H, Ashihara E, et al
  • Organizer: The 55^<th> Annual Meeting of American Society of Hematology
  • Place of Presentation: New Orleans, USA
• [Presentation] Cytokine-STATs signaling upregulate C/EBPβin BCR-ABL+ leukemic cells independently from BCR-ABL/JAK-STAT pathway (2013)
  • Author(s): Yokota A, Hirai H, Ashihara E, et al
  • Organizer: The 55^<th> Annual Meeting of American Society of Hematology
  • Place of Presentation: New Orleans, USA
• [Presentation] Microglia-like monocytic cells derived from bone marrow cells phagocytose amyloid-βand facilitate phagocytosis of amyloid-βby resident microglia (2013)
  • Author(s): Takata K, Ashihara E, et al
  • Organizer: The 55^<th> Annual Meeting of American Society of Hematology
  • Place of Presentation: New Orleans, USA
• [Presentation] Myeloid-derived suppressor cells have regulatory roles in mouse collagen-induced arthritis (2013)
  • Author(s): Fujii W, Ashihara E, Kawahito Y, et al
  • Organizer: The Annual European Congress of Rheumatology
  • Place of Presentation: Madrid, Spain
• [Presentation] Hypoxia-adapted myeloma cells possess stem cell-like character. (2013)
  • Author(s): 1. Ashihara E, Nakagawa Y, Yao H, Yokota A, Miura Y, Takata K, Kitamura Y, Hirai H, Maekawa T.
  • Organizer: The 55th Annual Meeting of American Society of Hematology.
  • Place of Presentation: New Orleans, USA
• [Presentation] Direct interaction with bone marrow mesenchymal stromal/stem cells is required for hematopoietic expansion by parathyroid hormone. (2013)
  • Author(s): 2. Yao H, Miura Y, Yoshioka S, Iwasa M, Sato A, Tamura A, Yokota A, Ashihara E, Ichinohe T, Hirai H, Maekawa T.
  • Organizer: The 55th Annual Meeting of American Society of Hematology.
  • Place of Presentation: New Orleans, USA
• [Presentation] Expression of C/EBPβ is involved in the exhaustion of hematopoietic stem cells through cell-intrinsic and cell-extrinsic mechanisms. (2013)
  • Author(s): 3. Tamura A, Hirai H, Hayashi Y, Yokota A, Sato A, Yao H, Yoshioka S, Iwasa M, Ashihara E, Miura Y, Maekawa T.
  • Organizer: The 55th Annual Meeting of American Society of Hematology.
  • Place of Presentation: New Orleans, USA
• [Presentation] Cytokine-STATs signalings upregulate C/EBPβ in BCR-ABL+ leukemic cells independently from BCR-ABL/JAK-STAT pathway. (2013)
  • Author(s): 4. Yokota A, Hirai H, Hayashi Y, Tamura A, Sato A, Yao H, Yoshioka S, Iwasa M, Ashihara E, Miura Y, Maekawa T.
  • Organizer: The 55th Annual Meeting of American Society of Hematology.
  • Place of Presentation: New Orleans, USA
• [Presentation] Microglia-like monocytic cells derived from bone marrow cells phagocytose amyloid-β and facilitate phagocytosis of amyloid-β by resident microglia. (2013)
  • Author(s): 5. Takata K, Toji Y, Kawanishi S, Takada T, Kitamura Y, Ashihara E.
  • Organizer: The 55th Annual Meeting of American Society of Hematology.
  • Place of Presentation: New Orleans, USA
• [Presentation] Myeloid-Derived Suppressor Cells have regulatory roles in mouse collagen-induced arthritis. (2013)
  • Author(s): 6. Fuji W, Ashihara E, Hirai H, Nagahara H, Fujioka K, Murakami K, Nakamura K, Seno T, Yamamoto A, Ishino H, Kohno M, Maekawa T, Kawahito Y.
  • Organizer: The Annual European Congress of Rheumatology.
  • Place of Presentation: Madrid, Spain.
• [Presentation] M-CSF刺激による造血幹細胞からのアミロイドβ貪食細胞への分化誘導. (2013)
  • Author(s): 高田和幸、河西翔平、杜氏裕美子、北村佳久、芦原英司
  • Organizer: 第75回日本血液学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Glioblastoma-derived exosomes targeting to cancer cells and their lipid characterization. (2013)
  • Author(s): Toda Y,Takata K, Kobayashi K, Hattori Y,Akaji K, Ashihara E.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] C/EBPβis upregulated through STAT5 and accelerates exhaustion of leukemic stem cells during BCR-ABL-mediated myeloid expansion (2012)
  • Author(s): Hayashi H, Hirai H, Ashihara E, et al
  • Organizer: The 54^<th> Annual Meeting of American Society of Hematology
  • Place of Presentation: Atlanta, USA
• [Presentation] C/EBPβ-mediated expansion of hematopoietic stem/progenitors precedes'Emergency'granulopoiesis induced by cadidemia (2012)
  • Author(s): Tamura A, Hirai H, Ashihara E, et al
  • Organizer: The 54^<th> Annual Meeting of American Society of Hematology
  • Place of Presentation: Atlanta, USA
• [Presentation] Myeloid-derived suppressor cells accumulated in spleens of mice with collagen-induced arthritis and inhibited immune response of CD4+ T cells (2012)
  • Author(s): Fujii W, Ashihara E, Kawahito Y, et al
  • Organizer: The 2012 American College of Rheumatology Annual meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] AV-65, a novel Wnt/β-catenin signal inhibitor, suppresses progression of multiple myeloma in a mouse model (2012)
  • Author(s): Ashihara E et al
  • Organizer: The 3dr JSH International Symposium
  • Place of Presentation: Kawagoe, Japan
• [Presentation] 新規Wnt/β-カテニン経路阻害剤AV65による多発性骨髄腫細胞の増殖抑制効果 (2012)
  • Author(s): 芦原英司、他
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] C/EBPβ Is Upregulated Through STAT5 and Accelerates Exhaustion of Leukemic Stem Cells During BCR-ABL-Mediated Myeloid Expansion. (2012)
  • Author(s): Hayashi H
  • Organizer: The 54th American Society of Hematology Annual Meeting and Exposition.
  • Place of Presentation: Atlanta, USA.
• [Presentation] C/EBPβ-Mediated Expansion of Hematopoietic Stem/Progenitors Precedes ‘Emergency’ Granulopoiesis Induced by Candidemia. (2012)
  • Author(s): Tamura A
  • Organizer: The 54th American Society of Hematology Annual Meeting and Exposition.
  • Place of Presentation: Atlanta, USA.
• [Presentation] M-CSFを処置した骨髄由来造血幹細胞によるアミロイドβの貪食. (2012)
  • Author(s): 杜氏 裕美子
  • Organizer: 第6回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 京都
• [Presentation] Myeloid-Derived Suppressor Cells Accumulated in Spleens of Mice with Collagen-Induced Arthritis and Inhibited Immune Response of CD4+ T cells. (2012)
  • Author(s): Fuji W
  • Organizer: The 2012 American College of Rheumatology Annual Meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] Parathyroid hormone stimulates mesenchymal stem cells to enhance expansion of hematopoietic cells. (2012)
  • Author(s): Yao H
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] Involvement of C/EBPβ in the pathogenesis of chronic phase chronic myeloid leukemia. (2012)
  • Author(s): Hayashi Y
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] BCR/ABLによるC/EBPbetaの発現亢進が慢性骨髄性白血病の骨髄球系造血亢進および病態進行をもたらす. (2012)
  • Author(s): 林 嘉宏
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] 新規Wnt/β-カテニン経路阻害剤AV65による多発性骨髄腫細胞の増殖抑制効果． (2012)
  • Author(s): 芦原英司
  • Organizer: ．第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] 原発性胆汁性肝硬変患者が保有するＨＬＡ抗体の特異性について． (2012)
  • Author(s): 万木紀美子
  • Organizer: 第21回日本組織適合性学会
  • Place of Presentation: 東京
• [Presentation] 細胞外HMGB1によるミクログリアのアミロイドβ1-40の貪食阻害作用. (2012)
  • Author(s): 高田哲也
  • Organizer: 次世代を担う創薬・医療薬理シンポジウム2012
  • Place of Presentation: 神戸
• [Presentation] Monocytes derived from bone marrow cells by macrophage colony-stimulating factor treatment effectively phagocytose amyloid-β. (2012)
  • Author(s): Kazuyuki Takata
  • Organizer: SEH-Society for Hematology and Stem Cells-41st Annual Scientific Meeting
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] BCR/ABLの白血病原性における転写因子C/EBPβの関与. (2012)
  • Author(s): 林 嘉宏
  • Organizer: 第16回日本がん分子標的治療学会
  • Place of Presentation: 北九州
• [Presentation] AV-65, a Novel Wnt/&#61538;-catenin Signal Inhibitor, Suppresses Progression of Multiple Myeloma in a Mouse Model. (2012)
  • Author(s): Ashihara E.
  • Organizer: The 3rd JSH International Symposium.
  • Place of Presentation: Kawagoe, Japan
• [Presentation] BCR/ABL-mediated myeloid expresion is promoted by C/EBPβ, a regulator of emergency granulopoiesis (2011)
  • Author(s): Hayashi Y, Hirai H, Ashihara E, et al
  • Organizer: The 53^<rd> Annual Meeting of American Society of Hematology
  • Place of Presentation: San Diego, USA
• [Presentation] Targeting the hedgehog signaling pathway in therapy-resistant Bcr-Abl1 positive leukemia with ponatinib (2011)
  • Author(s): Tauchi T, Ashihara E, et al
  • Organizer: The 53^<rd> Annual Meeting of American Society of Hematology
  • Place of Presentation: San Diego, USA
• [Presentation] A microfluidic device to mimic the bone marrow environment : real-time observation of the leukemic cell behavior (2011)
  • Author(s): Munaka T, Ashihara E, et al
  • Organizer: The 15^<th> International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Seattle, USA
• [Presentation] BCR/ABL-mediated myeloid expresion is promoted by C/EBP beta, a regulator of emergency granulopoiesis. (2011)
  • Author(s): Hayashi Y, Hirai H, Ashihara E, et al.
  • Organizer: 第53回アメリカ血液学会
  • Place of Presentation: 米国、サンディエゴ
• [Presentation] Combined effects of a pan-Abl1 kinase inhibitor, ponatinib and dasatinib against T315I mutant forms of Bcr-Abl1: in vitro and in vivo studies (2011)
  • Author(s): Tauchi T, Ashihara E, Ohyashiki K, et al.
  • Organizer: 第53回アメリカ血液学会
  • Place of Presentation: 米国、サンディエゴ
• [Presentation] Targeting the hedgehog signaling pathway in therapy-resistant Bcr-Abl1 positive leukemia with ponatinib. (2011)
  • Author(s): Katagiri S, Ashihara E, Ohyashiki K, et al.
  • Organizer: 第53回アメリカ血液学会
  • Place of Presentation: 米国、サンディエゴ
• [Presentation] A microfluidic device to mimic the bone marrow environment: real-time observation of the leukemic cell behavior. (2011)
  • Author(s): Munaka T, Ashihara E, Maekawa T, et al.
  • Organizer: The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences.
  • Place of Presentation: 米国、シアトル
• [Presentation] Future prospect of RNA interference for cancer therapies. (2011)
  • Author(s): Ashihara E.
  • Organizer: The12th Congress of the Asian Society of Transplantation(招待講演)
  • Place of Presentation: 韓国、ソウル
• [Presentation] Galectin-3 induced by leukemia microenvironment promotes drug resistance and BM lodgment in CML. (2011)
  • Author(s): Yamamoto M、Kuroda J, Ashjhara E, et al.
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
• [Presentation] Combined effects of the hedgehog inhibitor LDE225 and nilotinib in a random mutagesesis screen. (2011)
  • Author(s): Tauchi T, Ashihara E, Ohyasiki K, et al.
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
• [Presentation] Involvement of C/EBPβ in BCR/ABL-dependent leukemogenesis. (2011)
  • Author(s): Hayashi Y, Hirai H, Ashihara E, et al.
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
• [Presentation] AV-65 inhibited the growth of myeloma cells by enhancing β-TrCP-mediated β-catenin ubiquitination. (2011)
  • Author(s): Yao H, Ashihara E, et al.
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
• [Presentation] AV-65 inhibited the growth of myeloma cells by enhancingβ-TrCP-mediatedβ-catenin ubiquitination (2011)
  • Author(s): Yao H, Ashihara E, et al
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-15
• [Presentation] Future prospect of RNA interference for cancer therapies (2011)
  • Author(s): Ashihara E
  • Organizer: The12^<th> Congress of the Asian Society of Transplantation
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2011-09-27
• [Presentation] がん細胞指向性を有するヒト星状膠芽腫細胞由来エキソソームの発見．
  • Author(s): 戸田侑紀、高田和幸、中川由布子、小林数也、服部恭尚、北村佳久、赤路健一、芦原英司．
  • Organizer: 第5回日本RNAi研究会
  • Place of Presentation: 広島
• [Book] Nmerging Trends in Cell and Gene Therapy (2013)
  • Author(s): Ashihara E, Maekawa T
  • Total Pages: 705
  • Publisher: Springer
• [Book] 多発性骨髄腫Updating.第4巻移植適応骨髄腫の治療.新規薬剤時代における移植適応患者の治療戦略 (2013)
  • Author(s): 芦原英司
  • Total Pages: 203
  • Publisher: 医療ジャーナル社
• [Book] 多発性骨髄腫 Updating．第4巻 移植適応骨髄腫の治療．~新規薬剤時代における移植適応患者の治療戦略~ (2013)
  • Author(s): 芦原英司．
  • Total Pages: 203
  • Publisher: 医療ジャーナル社
• [Book] RNA Interference for Oncology: Clinical Prospects Beyond the Hype. In: Emerging Trends in Cell and Gene Therapy. (2013)
  • Author(s): Ashihara E, Maekawa T.
  • Total Pages: 705
  • Publisher: Springer
• [Book] Development of Cell Therapeutic Strategies for Alzheimer’s Disease Using Animal Models. Animal Models in Human Disease (2013)
  • Author(s): Takata K
  • Publisher: Nova Science Publishers
• [Book] New concept of limb salvage surgery in musculoskeletal sarcoma with acridine orange therapy. (2012)
  • Author(s): Kusuzaki K
  • Total Pages: 14
  • Publisher: Nova Science Publishers
• [Book] RAN医学・医療―新たな診断・治療を拓く― (2011)
  • Author(s): 芦原英司、前川平
  • Total Pages: 608
  • Publisher: 医歯薬出版
• [Book] RAN医学・医療―新たな診断・治療を拓く― (2011)
  • Author(s): 芦原英司、前川 平
  • Total Pages: 608
  • Publisher: 医歯薬出版
• [Remarks]
  • URL: http://labo.kyoto-phu.ac.jp/seiri/seiri-j.html
• [Remarks] 京都薬科大学・生命薬科学系 病態生理学分野
  • URL: http://www.kyoto-phu.ac.jp/labo/seiri/seiri-j.html
• [Patent(Industrial Property Rights)] マイクロ反応装置を用いた細胞運動評価システム (2012)
  • Inventor(s): 務中達也、阿部浩久、叶井正樹、前 川 平、木村晋也、芦 原英司、庄子習一
  • Industrial Property Rights Holder: 株式会社島津製作所 国立大学法人 京都大学 学校法人早稲田大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-12-28
  • Application Year: 2012
  • Overseas
• [Patent(Industrial Property Rights)] 細胞選別器及び細胞選別方法 (2011)
  • Inventor(s): 務中達也、阿部浩久、叶井正樹、前 川 平、木村晋也、芦原英司
  • Industrial Property Rights Holder: 株式会社島津製作所 国立大学法人 京都大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2011-210160
  • Filing Date: 2011-09-27
  • Application Year: 2011
  • Overseas