Analysis of leukemic microenvironment in RUNX1/EVI1 mouse model

• Project/Area Number: 23591406
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Dokkyo Medical University
• Principal Investigator: NAKAMURA Yuka 獨協医科大学, 医学部, 講師 (80364595)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 白血病モデルマウス / 白血病幹細胞 / 骨髄微小環境 / ニッチ因子

Research Abstract

Mice transplanted with RUNX1/EVI1-expressed bone marrow cells showed increased number of platelets and splenomegaly. In these mice increased number of megakaryocyte was recognized in bone marrow and spleen. RUNX1/EVI1 enhanced colony replating activity of hematopoietic cells. The expression level of Skp2, which is one of a target genes of RUNX1/EVI1, was decreased in colony forming cells gradually. Some mice developed leukemia after transplantation. c-Kit-positive leukemic cells invaded bone marrow, spleen and liver.

Research Products

• [Journal Article] Overexpression of MIR9 indicates poor prognosis in acute lymphoblastic leukemia (2013)
  • Author(s): Sugita F, Maki K, Nakamura Y, Sasaki K, Mitani K
  • Journal Title: Leuk Lymphoma
  • Peer Reviewed
• [Journal Article] Fadd and Skp2 are possible downstream targets of RUNX1-EVI1 (2013)
  • Author(s): Maki K, Sugita F, Nakamura Y, Sasaki K, Mitani K
  • Journal Title: Int J Hematol Volume: 97(1) Pages: 83-91
  • NAID: 10031144638
  • Peer Reviewed
• [Journal Article] Enhanced Angpt1/Tie2 signaling affects the differentiation and long-term repopulation ability of hematopoietic stem cells (2013)
  • Author(s): Ikushima YM, Arai F, Nakamura Y, Hosokawa K, Kubota Y, Hirashima M, Toyama H, Suda T
  • Journal Title: Biochem Biophys Res Commun Volume: 430(1) Pages: 20-5
  • Peer Reviewed
• [Journal Article] Acute myeloid leukemia with t(7;21)(q11.2;q22) expresses a novel, reversed-sequence RUNX1-DTX2 chimera (2012)
  • Author(s): Maki K, Sasaki K, Sugita F, Nakamura Y, Mitani K
  • Journal Title: Int J Hematol Volume: 96(2) Pages: 268-73
  • NAID: 10031043088
  • Peer Reviewed
• [Journal Article] Aberrant expression of MIR9 indicates poor prognosis in acute myeloid leukaemia (2012)
  • Author(s): Maki K, Yamagata T, Sugita F, Nakamura Y, Sasaki K, Mitani K
  • Journal Title: Br J Haematol Volume: 158(2) Pages: 283-5
  • Peer Reviewed