| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
CMV pp65 specific T cells are able to be detected in magnetically separated CD8+ T cells by IFNg ELISPOT assay with epitope peptide pulsed CD40-activated B cells, but not with peptide alone or unseparated PBMCs. Under written informed consent, PBMCs were collected and stored from five CMV sero-positive HLA identical donors and their recipients. After identification of unknown CMV pp65 epitopes, IFNg ELISPOT assay with the epitope peptides will be conducted.
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