Functional evaluation of umbilical cord stem cells as a source for cell-based therapy in children
| Project/Area Number |
23591428
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Taro 日本大学, 医学部, 教授 (50366580)
YAGASAKI Hiroshi 日本大学, 医学部, 助教 (90378141)
ISHIGE Mika 日本大学, 医学部, 助教 (90420950)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 造血幹細胞移植学 / 再生医学 / 移植・再生医療 / 胎児由来幹細胞 / 間葉系幹細胞 / 臍帯血 |
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| Research Abstract |
We examined phenotypic and functional analysis of several stem cells isolated from human embryonic tissue. We found that p75NTR-positive cells, specifically localized sub endothelium of umbilical artery, expressed several neural crest markers and differentiated into neuronal and glial cells. Mesenchymal stem cells (MSCs) derived from Wharton's jelly of umbilical cord (WJ-MSCs), epithelium of placental amnion (AECs), and mesenchyme of placental amnion (AMCs) were prepared from same donors and compared their cellular function. WJ-MSCs characteristically suppressed peripheral blood lymphocyte proliferation, whereas AECs dominantly supported human hematopoietic stem cells in vitro. Co-transplantation of WJ-MSCs, AECs, and AMCs with human umbilical cord blood CD34-positive cells into NOD-SCID mice promoted the engraftment of human blood cells. These results suggest that these embryonic tissue-derived stem cells are useful to inhibit graft failure following cord blood transplantation.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Diabetes caused by Kir6.2 mutation: successful treatment with oral glibenclamide switched from continuous subcutaneous insulin infusion in the early phase of the disease.2012
Author(s)
Nagano N, Urakami T, Mine Y, Watanaabe H, Yoshida A, Suzuki J, Saito H, Ishige M, Takahashi S, Mugishima H, Yorifuji T.
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Journal Title
Pediatric International
Volume: 54
Issue: 2
Pages: 277-279
DOI
Related Report
Peer Reviewed
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[Journal Article] Treatment responses for disseminated intravascular coagulation in 25 children treated with recombinant thrombomodulin: a single institution experience.2012
Author(s)
Yagasaki H, Kato M, Shimozawa K, Hirai M, Nishikawa E, Okuma H, Ishii W, Matsumura M, Yonezawa R, Yoshikawa K, Shichino H, Chin M, Mugishima H
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Journal Title
Thrombosis Research
Volume: 130
Issue: 6
Pages: e289-e293
DOI
Related Report
Peer Reviewed
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[Presentation] 臍帯血移植と患者生存に与える因子の解析2012
Author(s)
山本法子, 小林寿美子, 星野茂角, 橋本安男, 田原佳幸, 田中博, 松本太郎, 幸道秀樹, 相澤信, 青木繁之, 麦島秀雄
Organizer
第34回日本造血細胞移植療学会総会
Place of Presentation
大阪
Related Report
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[Presentation] 臍帯血有核細胞数の保存基準改訂に伴う保存及び出庫(供給)数の変化について2012
Author(s)
星野茂角, 橋本安男, 田原佳幸, 山本法子, 田中博, 小林寿美子, 高橋敦子, 幸道秀樹, 相澤信, 松本太郎, 麦島秀雄
Organizer
第34回日本造血細胞移植療学会総会
Place of Presentation
大阪
Related Report
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