| Project/Area Number |
23591428
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Taro 日本大学, 医学部, 教授 (50366580)
YAGASAKI Hiroshi 日本大学, 医学部, 助教 (90378141)
ISHIGE Mika 日本大学, 医学部, 助教 (90420950)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 造血幹細胞移植学 / 再生医学 / 移植・再生医療 / 胎児由来幹細胞 / 間葉系幹細胞 / 臍帯血 |
|---|
| Research Abstract |
We examined phenotypic and functional analysis of several stem cells isolated from human embryonic tissue. We found that p75NTR-positive cells, specifically localized sub endothelium of umbilical artery, expressed several neural crest markers and differentiated into neuronal and glial cells. Mesenchymal stem cells (MSCs) derived from Wharton's jelly of umbilical cord (WJ-MSCs), epithelium of placental amnion (AECs), and mesenchyme of placental amnion (AMCs) were prepared from same donors and compared their cellular function. WJ-MSCs characteristically suppressed peripheral blood lymphocyte proliferation, whereas AECs dominantly supported human hematopoietic stem cells in vitro. Co-transplantation of WJ-MSCs, AECs, and AMCs with human umbilical cord blood CD34-positive cells into NOD-SCID mice promoted the engraftment of human blood cells. These results suggest that these embryonic tissue-derived stem cells are useful to inhibit graft failure following cord blood transplantation.
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