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Expression of IL17F induced by a novel cytokine IL-33

Research Project

Project/Area Number 23591455
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionUniversity of Tsukuba

Principal Investigator

KAWAGUCHI MIO  筑波大学, 医学医療系, 講師 (50365748)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords気管支喘息 / IL-17F / IL-33 / 気道上皮細胞 / 喘息
Research Abstract

IL-17F is also involved in the pathogenesis of asthma. However, the regulatory mechanisms of IL-17F expression remain to be defined. Therefore we investigated the expression of IL-17F by IL-33 in bronchial epithelial cells, and its signaling mechanisms. IL-33 significantly induced IL-17F gene and protein expression. The receptor for IL-33, ST2, was expressed in bronchial epithelial cells. Among MAP kinases, IL-33 phosphorylated ERK1/2. It was inhibited by the pretreatment of anti-ST2 neutralizing (blocking) Abs. MEK inhibitor significantly blocked IL-17F production. Moreover, IL-33 phosphorylated MSK1, and MEK inhibitor diminished its phosphorylation. Finally, MSK1 inhibitors and transfection of the siRNAs targeting MSK1 significantly blocked IL-17F expression. IL-33 induces IL-17F via ST2-ERK1/2-MSK1 signaling pathway in bronchial epithelial cells. These data suggest that the IL-33/IL-17F axis is involved in allergic airway inflammation, and may be a novel therapeutic target.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 2 results) Presentation (10 results) (of which Invited: 1 results)

  • [Journal Article] Potential involvement of IL-17F in asthma2014

    • Author(s)
      Ota K, Kawaguchi M, Matsukura S, Kurokawa M, Kokubu F, Fujita J, Morishima Y, Huang SK, Ishii Y, HSatoh H, Nobuyuki Hizawa N
    • Journal Title

      Journal of Immunology Research

      Volume: Vol.2014 Pages: 1-8

    • DOI

      10.1155/2014/602846

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interleukin-33 induces interleukin-17F in bronchial epithelial cells2012

    • Author(s)
      Fujita J, Kawaguchi M, Kokubu F, Ohara G, Ota K, Huang SK, Morishima Y, IshiiY, Satoh H, Sakamoto T, Hizawa N
    • Journal Title

      Allergy

      Volume: 67 Issue: 6 Pages: 744-750

    • DOI

      10.1111/j.1398-9995.2012.02825.x

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] [アレルギーに対するサイトカイン]IL-172012

    • Author(s)
      川口未央、太田恭子、藤田純一、檜澤伸之
    • Journal Title

      アレルギー・免疫

      Volume: Vol.19 Pages: 1892-1898

    • Related Report
      2013 Final Research Report
  • [Journal Article] [難治性喘息研究の新展開]サイトカインからみた難治性喘息2012

    • Author(s)
      川口未央、太田恭子、藤田純一、檜澤伸之
    • Journal Title

      呼吸器内科

      Volume: Vol.21 Pages: 48-53

    • Related Report
      2013 Final Research Report
  • [Journal Article] 【アレルギーに対するサイトカイン】 IL-172012

    • Author(s)
      川口 未央, 太田 恭子, 藤田 純一, 檜澤 伸之
    • Journal Title

      アレルギー・免疫

      Volume: 19 Pages: 1892-1898

    • Related Report
      2012 Research-status Report
  • [Journal Article] 【難治性喘息研究の新展開】 サイトカインからみた難治性喘息2012

    • Author(s)
      川口 未央, 太田 恭子, 藤田 純一, 檜澤 伸之
    • Journal Title

      呼吸器内科

      Volume: 21 Pages: 48-53

    • Related Report
      2012 Research-status Report
  • [Journal Article] IL-17AとIL17Fにおけるシグナル伝達2011

    • Author(s)
      藤田純一、川口未央、檜澤伸之
    • Journal Title

      アレルギー・免疫

      Volume: Vol.55 Pages: 585-588

    • NAID

      40018868199

    • Related Report
      2013 Final Research Report
  • [Journal Article] IL-17AとIL-17Fにおけるシグナル伝達2011

    • Author(s)
      藤田純一、川口未央他
    • Journal Title

      臨床免疫・アレルギー科

      Volume: 55 Pages: 585-588

    • NAID

      40018868199

    • Related Report
      2011 Research-status Report
  • [Presentation] Expression of IL-17F in bronchial epithelial cells2013

    • Author(s)
      Kawaguchi M, Fujita J, Ota K, Kurishima K, Morishima Y, Ishii Y, Sakamoto T, Satoh H, Hizawa N
    • Organizer
      KAPARD-KAAACI & West Pacific Allergy Symposium Joint International Congress
    • Place of Presentation
      Grand Hilton Seoul(ソウル)韓国
    • Year and Date
      2013-05-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Expression of IL-17F in bronchial epithelial cells2013

    • Author(s)
      Mio Kawaguchi, Junichi Fujita, Kyoko Ota, Koichi Kurishima, Yuko Morishima, Yukio Ishii, Toru Sakamoto, Hiroaki Satoh, Nobuyuki Hizawa
    • Organizer
      KAPARD-KAAACI & West Pacific Allergy Symposium Joint International Congress
    • Place of Presentation
      Grand Hilton Seoul(ソウル)韓国
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] IL-33 Induces IL-17F In Bronchial Epithelial Cells2012

    • Author(s)
      Fujita J, Kawaguchi M, Kokubu F, Matsukura S, Kurokawa M, Nozato K, Ano S,Kaneko Y, Masuko H, Yamadori T, Yageta Y, Morishima Y, Ishii Y, Sakamoto T, Satoh H, Hizawa N
    • Organizer
      International Conference of American Thoracic Society
    • Place of Presentation
      モスコー二センター、サンフランシスコ、米国
    • Year and Date
      2012-05-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 気道上皮細胞からのIL-17F産生2012

    • Author(s)
      藤田純一,川口未央,國分二三男,松倉聡,黒川真嗣,野里恭子,阿野哲士,金子美子,増子裕典,山鳥忠宏,森島祐子,石井幸雄,坂本透,足立満,檜澤伸之
    • Organizer
      第52回日本呼吸器学会学術講演会
    • Place of Presentation
      神戸コンベションセンター、神戸
    • Year and Date
      2012-04-22
    • Related Report
      2013 Final Research Report
  • [Presentation] IL-33 Induces IL-17F In Bronchial Epithelial Cells2012

    • Author(s)
      Fujita J, Kawaguchi M, Kokubu F, Matsukura S, Kurokawa M, Nozato K, Ano S,Kaneko Y, Masuko H, Yamadori T, Yageta Y, Morishima Y, Ishii Y, Sakamoto T, Satoh H, Hizawa N
    • Organizer
      International Conference of American Thoracic Society
    • Place of Presentation
      米国
    • Related Report
      2012 Research-status Report
  • [Presentation] 気道上皮細胞からのIL-17F産生2012

    • Author(s)
      藤田 純一, 川口 未央, 國分 二三男, 松倉 聡, 黒川 真嗣, 野里 恭子, 阿野 哲士, 金子 美子, 増子 裕典, 山鳥 忠宏, 森島 祐子, 石井 幸雄, 坂本 透, 足立 満, 檜澤伸之
    • Organizer
      第52回日本呼吸器学会学術講演会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Presentation] 気道上皮細胞におけるIL-17Fの発現とそのメカニズム2011

    • Author(s)
      川口未央、藤田純一、國分二三男、松倉聡、黒川真嗣、野里恭子、森島祐子、石井幸雄、佐藤浩昭、坂本透、足立満、檜澤伸之
    • Organizer
      第15回アレルギー・気道上皮細胞研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-12-10
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 気道上皮細胞からのIL-33によるIL-17F産生:MSK1の関与2011

    • Author(s)
      藤田純一、川口未央、國分二三男、松倉聡、黒川真嗣、野里恭子、阿野哲士、金子美子、増子裕典、山鳥忠宏、森島祐子、石井幸雄、坂本透、足立満、檜澤伸之
    • Organizer
      第61回日本アレルギー学会秋季学術大会
    • Place of Presentation
      グランドプリンスホテル新高輪、東京
    • Year and Date
      2011-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 気道上皮細胞からのIL-33によるIL-17F産生2011

    • Author(s)
      藤田純一、川口未央、國分二三男、松倉聡、黒川真嗣、野里恭子、阿野哲士、金子美子、増子裕典、山鳥忠宏、森島祐子、石井幸雄、坂本透、足立満、檜澤伸之
    • Organizer
      第8回北関東信越呼吸器フォーラム
    • Place of Presentation
      東京
    • Year and Date
      2011-07-09
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] 気道上皮細胞からのIL-33によるIL-17F産生:MSK1の関与2011

    • Author(s)
      藤田純一
    • Organizer
      第61回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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